Alpha-amylase variants and polynucleotides encoding same

ABSTRACT

The present invention relates to alpha-amylase variants of a parent alpha-amylase wherein said alpha-amylase variants have an improved wash performance as compared to said parent alpha-amylase. The invention also relates to compositions comprising the variants of the invention, uses of said variants and methods of producing said variants.

REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing in computer readable form,which is incorporated herein by reference.

BACKGROUND OF THE INVENTION Field of the Invention

The present invention relates to alpha-amylase variants, polynucleotidesencoding the variants, methods of producing the variants, and methods ofusing the variants.

Description of the Related Art

Alpha-amylases (α-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.) are agroup of enzymes that hydrolyzes starch, glycogen, and other relatedpolysaccharides by cleaving the internal α-1,4-glucosidic bonds. It hasbeen used for many years been in, e.g., laundry where is it well-knownthat alpha-amylases have a beneficial effect in removal of starchcontaining, or starch-based, stains. However, in other commercialapplications the enzyme has become important, such as in the initialstages (liquefaction) of starch processing, in textile desizing, inalcohol production and as cleaning agents in detergent compositions.

In recent years there has been a desire to improve the properties ofvarious amylases. In particular, the object of reducing the temperatureof the laundry in order to reduce the energy consumption has been ofprimary focus when referring to the household care sector. Thus, manyefforts have been put into finding improved alpha-amylase variants.

Regardless, there remains a need for alpha-amylase variants that possessaltered properties and offer improved performance in various industrialapplications. Thus, the present invention provides such further improvedalpha-amylase variants with improved properties compared to its parent.

It is an object of the present invention to provide polypeptides havingalpha-amylase activity (alpha-amylases) which have improved washperformance, especially for use in automatic washing machines and/ordishwashers.

SUMMARY OF THE INVENTION

The present invention relates to an alpha-amylase variant of a parentalpha-amylase comprising a modification at one or more positionscorresponding to positions: H1, H2, G7, I9, Q11, N16, N19, N25, A37,W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90, Q98, V104,G109, A111, F113, R116, Q118, Q125, R127, E130, S132, G133, T134, Y135,Q136, A139, G142, N144, G149, R158, Y160, D163, W167, Q169, S170, R171,Q172, L173, A174, N175, R176, Y178, R181, G182, A186, W187, N195, M202,Y203, A204, V206, D209, H210, E212, T227, L235, V238, M246, Q256, K259,V264, A265, E266, Y267, K269, N270, L272, G273, A274, L275, E276, W284,M286, V291, L293, Y295, Y298, Q299, N302, S303, S304, N306, R310, N311,N314, G315, L317, Q319, R320, S323, F328, G337, A339, Q345, D357, Q365,P377, D375, Q385, K391, Q395, R400, D406, W408, V410, S431, G435, W439,R444, Q445, R458, N465, Q466, W469, F473, G476 and V481, using SEQ IDNO: 1 for numbering, and wherein said variant has alpha-amylase activityand wherein said variant has at least 60%, at least 65%, at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, or at least 99%, but less than100% sequence identity to the polypeptide of SEQ ID NOs: 1-17, andwherein the said variant has improved wash performance compared to saidparent alpha-amylase and wherein said parent alpha-amylase has aminoacid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.

The present invention also relates to polynucleotides encoding thevariants; nucleic acid constructs, vectors, and host cells comprisingthe polynucleotides; and methods of producing the variants.

The present invention also relates to methods of improving washperformance of a parent alpha-amylase.

DETAILED DESCRIPTION OF THE INVENTION Definitions

In accordance with this detailed description, the following definitionsapply. Note that the singular forms “a,” “an,” and “the” include pluralreferences unless the context clearly dictates otherwise.

Reference to “about” a value or parameter herein includes aspects thatare directed to that value or parameter per se. For example, descriptionreferring to “about X” includes the aspect “X”.

Unless defined otherwise or clearly indicated by context, all technicaland scientific terms used herein have the same meaning as commonlyunderstood by one of ordinary skill in the art to which this inventionbelongs.

The term “alpha-amylase” is synonymous with the term “polypeptideshaving alpha-amylase activity”. “Alpha-amylase activity” means theactivity of alpha-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.1, whichconstitute a group of enzymes, catalyzing hydrolysis of starch and otherlinear and branched 1,4-glucosidic oligo- and polysaccharides. Thus, theterm “alpha-amylase” as used herein, refers to an enzyme that hasalpha-amylase activity (Enzyme Class; EC 3.2.1.1) that hydrolyses alphabonds of large, alpha-linked polysaccharides, such as starch andglycogen, yielding glucose and maltose. The terms “alpha-amylase” and“amylase” may be used interchangeably and constitute the same meaningand purpose herein. For purposes of the present invention, alpha-amylaseactivity is determined according to the procedure described under thesub-heading Methods, below. In one aspect, the alpha-amylases of thepresent invention have at least 20%, e.g., at least 40%, at least 50%,at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, orat least 100% of the alpha-amylase activity of one or more of thepolypeptides of SEQ ID NOs:1-17.

The term ‘amino acid’ as used herein, refers to the standard twentygenetically-encoded amino acids and their corresponding stereoisomers inthe ‘d’ form (as compared to the natural ‘1’ form), omega-amino acidsother naturally-occurring amino acids, unconventional amino acids (e.g.α,α-disubstituted amino acids, N-alkyl amino acids, etc.) and chemicallyderivatised amino acids. Chemical derivatives of one or more amino acidsmay be achieved by reaction with a functional side group. Suchderivatised molecules include, for example, those molecules in whichfree amino groups have been derivatised to form amine hydrochlorides,p-toluene sulphonyl groups, carboxybenzoxy groups, t-butyloxycarbonylgroups, chloroacetyl groups or formyl groups. Free carboxyl groups maybe derivatised to form salts, methyl and ethyl esters or other types ofesters and hydrazides. Free hydroxyl groups may be derivatised to formO-acyl or O-alkyl derivatives. Also included as chemical derivatives arethose peptides which contain naturally occurring amino acid derivativesof the twenty standard amino acids. For example: 4-hydroxyproline may besubstituted for proline; 5-hydroxylysine may be substituted for lysine;3-methylhistidine may be substituted for histidine; homoserine may besubstituted for serine and ornithine for lysine. Derivatives alsoinclude peptides containing one or more additions or deletions as longas the requisite activity is maintained. Other included modificationsare amidation, amino terminal acylation (e.g. acetylation orthioglycolic acid amidation), terminal carboxylamidation (e.g. withammonia or methylamine), and the like terminal modifications.

When an amino acid is being specifically enumerated, such as ‘alanine’or ‘Ala’ or ‘A’, the term refers to both I-alanine and d-alanine unlessexplicitly stated otherwise. Other unconventional amino acids may alsobe suitable components for polypeptides of the present invention, aslong as the desired functional property is retained by the polypeptide.For the peptides shown, each encoded amino acid residue, whereappropriate, is represented by a single letter designation,corresponding to the trivial name of the conventional amino acid. In oneembodiment, the polypeptides of the invention comprise or consist ofI-amino acids.

The term “catalytic domain” means the region of an enzyme containing thecatalytic machinery of the enzyme.

The term “cDNA” means a DNA molecule that can be prepared by reversetranscription from a mature, spliced, mRNA molecule obtained from aeukaryotic cell. cDNA lacks intron sequences that may be present in thecorresponding genomic DNA. The initial, primary RNA transcript is aprecursor to mRNA that is processed through a series of steps, includingsplicing, before appearing as mature spliced mRNA.

The term “coding sequence” means a polynucleotide, which directlyspecifies the amino acid sequence of its polypeptide product. Theboundaries of the coding sequence are generally determined by an openreading frame, which usually begins with the ATG start codon oralternative start codons such as GTG and TTG and ends with a stop codonsuch as TAA, TAG, and TGA. The coding sequence may be a DNA, cDNA,synthetic, or recombinant polynucleotide.

The term “control sequences” means all components necessary for theexpression of a polynucleotide encoding a variant of the presentinvention. Each control sequence may be native or foreign to thepolynucleotide encoding the variant or native or foreign to each other.Such control sequences include, but are not limited to, a leader,polyadenylation sequence, propeptide sequence, promoter, signal peptidesequence, and transcription terminator. At a minimum, the controlsequences include a promoter, and transcriptional and translational stopsignals. The control sequences may be provided with linkers for thepurpose of introducing specific restriction sites facilitating ligationof the control sequences with the coding region of the polynucleotideencoding a variant.

The term “corresponding to” as used herein, refers to a way ofdetermining the specific amino acid of a sequence wherein reference ismade to a specific amino acid sequence. E.g. for the purposes of thepresent invention, when references are made to specific amino acidpositions, the skilled person would be able to align another amino acidsequence to said amino acid sequence that reference has been made to, inorder to determine which specific amino acid may be of interest in saidanother amino acid sequence. Alignment of another amino acid sequencewith e.g. the sequence as set forth in SEQ ID NO: 1, or any othersequence listed herein, has been described elsewhere herein. Alignmentof another amino acid sequence with e.g. the sequence as set forth inSEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or 17,or any other sequence listed herein, has been described elsewhereherein. Alternative alignment methods may be used and are well-known forthe skilled person.

The term “dish washing composition” as used herein, refers to all formsof compositions for cleaning hard surfaces. The present invention is notrestricted to any particular type of dish wash composition or anyparticular detergent. Thus, in one embodiment, the dish washingcomposition is a liquid dish washing composition, a powder dish washingcomposition, wherein the composition may optionally be in the form of aunit dose.

The term “enzyme detergency benefit” used herein, refers to theadvantageous effect an enzyme may add to a detergent compared to thesame detergent without the enzyme. Important detergency benefits whichcan be provided by enzymes are stain removal with no or very littlevisible soils after washing and/or cleaning, prevention or reduction ofre-deposition of soils released in the washing process (an effect thatalso is termed anti-redeposition), restoring fully or partly thewhiteness of textiles which originally were white but after repeated useand wash have obtained a greyish or yellowish appearance (an effect thatalso is termed whitening). Textile care benefits, which are not directlyrelated to catalytic stain removal or prevention of re-deposition ofsoils, are also important for enzyme detergency benefits. Examples ofsuch textile care benefits are prevention or reduction of dye transferfrom one fabric to another fabric or another part of the same fabric (aneffect that is also termed dye transfer inhibition oranti-backstaining), removal of protruding or broken fibers from a fabricsurface to decrease pilling tendencies or remove already existing pillsor fuzz (an effect that also is termed anti-pilling), improvement of thefabric-softness, colour clarification of the fabric and removal ofparticulate soils which are trapped in the fibers of the fabric orgarment. Enzymatic bleaching is a further enzyme detergency benefitwhere the catalytic activity generally is used to catalyze the formationof bleaching component such as hydrogen peroxide or other peroxides.

The term “expression” as used herein, refers to any step involved in theproduction of a variant including, but not limited to, transcription,post-transcriptional modification, translation, post-translationalmodification, and secretion.

The term “expression vector” as used herein, refers to a linear orcircular DNA molecule that comprises a polynucleotide encoding a variantand is operably linked to control sequences that provide for itsexpression.

The term “fragment” means a polypeptide having one or more (several)amino acids deleted from the amino and/or carboxyl terminus of a maturepolypeptide; wherein the fragment has alpha-amylase activity.

The term “high stringency conditions” means for probes of at least 100nucleotides in length, prehybridization and hybridization at 42° C. in5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon spermDNA, and 50% formamide, following standard Southern blotting proceduresfor 12 to 24 hours. The carrier material is finally washed three timeseach for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

The term “hard surface cleaning” as used herein, refers to cleaning ofhard surfaces wherein hard surfaces may include floors, tables, walls,roofs etc. as well as surfaces of hard objects such as cars (car wash)and dishes (dish wash). Dish washing includes but are not limited tocleaning of plates, cups, glasses, bowls, cutlery such as spoons,knives, forks, serving utensils, ceramics, plastics, metals, china,glass and acrylics.

The term “improved property” is defined herein as a characteristicassociated with a variant that is improved compared to the parentalpha-amylase. Such improved properties include, but are not limited to,increased amylolytic activity, increased catalytic efficiency, increasedcatalytic rate, increased chemical stability, increased oxidationstability, increased pH activity, increased pH stability, increasedrelative specific activity, increased specific activity, increasedsubstrate binding, increased substrate cleavage, increased substratespecificity, increased substrate stability, increased surfaceproperties, increased thermal activity, increased thermostability,increased wash performance such as soil removal performance e.g.performance to starch-containing soils, stain removal, anti-greying,stability e.g. thermostability, pH stability, or stability in thepresence of builders, including chelant, stability in powder, liquid orgel detergent formulation or dishwashing composition, alteredtemperature-dependent performance and activity profile, pH activity,substrate specificity, product specificity, and chemical stability. Theimproved property may be any of those herein defined and described, suchas wash performance.

The term “improved wash performance” is defined herein as displaying amodification of the wash performance of an amylase of the presentinvention relative to the wash performance of the parent alpha-amylaseof SEQ ID NO: 1 or SEQ ID NO: 2. The modification may e.g. be seen asincreased stain removal. The wash performance is improved if theImprovement Factor (IF) is at least 1.1, at least 1.2, at least 1.3.

The term “isolated” as used herein, refers to a substance in a form orenvironment which does not occur in nature. Non-limiting examples ofisolated substances include (1) any non-naturally occurring substance,(2) any substance including, but not limited to, any enzyme, variant,nucleic acid, protein, peptide or cofactor, that is at least partiallyremoved from one or more or all of the naturally occurring constituentswith which it is associated in nature; (3) any substance modified by thehand of man relative to that substance found in nature; or (4) anysubstance modified by increasing the amount of the substance relative toother components with which it is naturally associated (e.g., multiplecopies of a gene encoding the substance; use of a stronger promoter thanthe promoter naturally associated with the gene encoding the substance).An isolated substance may be present in a fermentation broth sample.

The term “isolated polynucleotide” means a polynucleotide that ismodified by the hand of man. In one aspect, the isolated polynucleotideis at least 1% pure, e.g., at least 5% pure, at least 10% pure, at least20% pure, at least 40% pure, at least 60% pure, at least 80% pure, atleast 90% pure, and at least 95% pure, as determined by agaroseelectrophoresis. The polynucleotides may be of genomic, cDNA, RNA,semisynthetic, synthetic origin, or any combinations thereof.

The term “mature polypeptide” as used herein, refers to means apolypeptide in its final form following translation and anypost-translational modifications, such as N-terminal processing,C-terminal truncation, glycosylation, phosphorylation, etc. It is knownin the art that a host cell may produce a mixture of two of moredifferent mature polypeptides (i.e., with a different C-terminal and/orN-terminal amino acid) expressed by the same polynucleotide.

The term “mature polypeptide coding sequence” as used herein, refers toa polynucleotide that encodes a mature polypeptide having alpha-amylaseactivity.

The term “modification”, in the context of the polypeptides of theinvention, means that one or more amino acids within the reference aminoacid sequence (i.e. SEQ ID NOs: 1 or 2) are altered by substitution witha different amino acid, by insertion of an amino acid or by deletion,preferably by at least two deletion. The terms “modification”,“alteration”, and “mutation” may be used interchangeably and constitutethe same meaning and purpose.

The term “medium stringency conditions” means for probes of at least 100nucleotides in length, prehybridization and hybridization at 42° C. in5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon spermDNA, and 35% formamide, following standard Southern blotting proceduresfor 12 to 24 hours. The carrier material is finally washed three timeseach for 15 minutes using 2×SSC, 0.2% SDS at 55° C.

The term “medium-high stringency conditions” means for probes of atleast 100 nucleotides in length, prehybridization and hybridization at42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denaturedsalmon sperm DNA, and 35% formamide, following standard Southernblotting procedures for 12 to 24 hours. The carrier material is finallywashed three times each for 15 minutes using 2×SSC, 0.2% SDS at 60° C.

The term “mutant” means a polynucleotide encoding a variant.

The term “nucleic acid construct” means a nucleic acid molecule, eithersingle- or double-stranded, which is isolated from a naturally occurringgene or is modified to contain segments of nucleic acids in a mannerthat would not otherwise exist in nature or which is synthetic. The termnucleic acid construct is synonymous with the term “expression cassette”when the nucleic acid construct contains the control sequences requiredfor expression of a coding sequence of the present invention.

The term “operably linked” means a configuration in which a controlsequence is placed at an appropriate position relative to the codingsequence of a polynucleotide such that the control sequence directs theexpression of the coding sequence.

The term “parent” or “parent alpha-amylase” means an alpha-amylase towhich a modification is made to produce the enzyme variants of thepresent invention. The parent may be a naturally occurring (wild-type)polypeptide or a variant thereof.

The term “sequence identity” as used herein refers to relatednessbetween two amino acid sequences or between two nucleotide sequences isdescribed by the parameter “sequence identity”.

For purposes of the present invention, the sequence identity between twoamino acid sequences is determined using the Needleman-Wunsch algorithm(Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implementedin the Needle program of the EMBOSS package (EMBOSS: The EuropeanMolecular Biology Open Software Suite, Rice et al., 2000, Trends Genet.16: 276-277), preferably version 5.0.0 or later. The parameters used maybe gap open penalty of 10, gap extension penalty of 0.5, and theEBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The outputof Needle labeled “longest identity” (obtained using the −nobriefoption) is used as the percent identity and is calculated as follows:

(Identical Residues×100)/(Length of Alignment Total Number of Gaps inAlignment)

Alternatively, the parameters used may be gap open penalty of 10, gapextension penalty of 0.5, and the EDNAFULL (EMBOSS version of NCBINUC4.4) substitution matrix. The output of Needle labeled “longestidentity” (obtained using the −nobrief option) is used as the percentidentity and is calculated as follows:

(Identical Deoxyribonucleotides×100)/(Length of Alignment−Total Numberof Gaps in Alignment)

The term “subsequence” as used herein, refers to a polynucleotide havingone or more (e.g., several) nucleotides absent from the 5′ and/or 3′ endof a mature polypeptide coding sequence; wherein the subsequence encodesa fragment having alpha-amylase activity.

The term “textile” refers to woven fabrics, as well as staple fibres andfilaments suitable for conversion to or use as yarns, woven, knit, andnon-woven fabrics. The term encompasses yarns made from natural, as wellas synthetic (e.g., manufactured) fibres. The term, “textile materials”is a general term for fibres, yarn intermediates, yarn, fabrics, andproducts made from fabrics (e.g., garments and other articles).

The term “textile care benefits”, as used herein, is defined as notbeing directly related to catalytic stain removal or prevention ofre-deposition of soils, are also important for enzyme detergencybenefits. Examples of such textile care benefits are prevention orreduction of dye transfer from one textile to another textile or anotherpart of the same textile (an effect that is also termed dye transferinhibition or anti-backstaining), removal of protruding or broken fibersfrom a textile surface to decrease pilling tendencies or remove alreadyexisting pills or fuzz (an effect that also is termed anti-pilling),improvement of the textile-softness, colour clarification of the textileand removal of particulate soils which are trapped in the fibers of thetextile. Enzymatic bleaching is a further enzyme detergency benefitwhere the catalytic activity generally is used to catalyze the formationof bleaching component such as hydrogen peroxide or other peroxides orother bleaching species.”

The terms “variant” or “polypeptide variant” or “polypeptide” or“alpha-amylase variant” when used in relation to a variant of thepresent invention, as used herein, refer to a polypeptide havingalpha-amylase activity comprising a modification, i.e., a substitution,insertion, and/or deletion, at one or more (e.g., several) positionsrelative to the ‘parent’ alpha-amylase of SEQ ID NOs:1 or 2. Asubstitution means replacement of the amino acid occupying a positionwith a different amino acid; a deletion means removal of the amino acidoccupying a position; and an insertion means adding an amino acidadjacent to and immediately following the amino acid occupying aposition The variants of the present invention have at least 20%, e.g.,at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, atleast 90%, at least 95%, or at least 100% of the alpha-amylase activityof the polypeptide of SEQ ID NOs: 1-17.

The term “very high stringency conditions” means for probes of at least100 nucleotides in length, prehybridization and hybridization at 42° C.in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmonsperm DNA, and 50% formamide, following standard Southern blottingprocedures for 12 to 24 hours. The carrier material is finally washedthree times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.

The term “very low stringency conditions” means for probes of at least100 nucleotides in length, prehybridization and hybridization at 42° C.in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmonsperm DNA, and 25% formamide, following standard Southern blottingprocedures for 12 to 24 hours. The carrier material is finally washedthree times each for 15 minutes using 2×SSC, 0.2% SDS at 45° C.

The term “wash performance” is defined herein as displaying analteration of the wash performance of an amylase of the presentinvention relative to the wash performance of the parent amylase of SEQID NO: 1 or the amylase of SEQ ID NO: 2. Improved wash performance maybe measured by comparing of the so-called Intensity value.

The term “wild-type alpha-amylase” as used herein refers to analpha-amylase expressed by a naturally occurring microorganism, such asa bacterium, yeast, or filamentous fungus found in nature.

The term “wash cycle” is defined herein with respect to dishwashing as awashing operation wherein dishware are exposed to the wash liquor for aperiod of time by circulating the wash liquor and spraying the washliquor onto the dishware in order to clean the dishware and finally thesuperfluous wash liquor is removed. A wash cycle may be repeated one,two, three, four, five or even six times at the same or at differenttemperatures. Hereafter the dishware is generally rinsed and dried. Oneof the wash cycles can be a soaking step, where the dishware is leftsoaking in the wash liquor for a period.

The term “wash liquor” is defined herein as the solution or mixture ofwater and detergent components.

The term “wash time” with respect to automatic dishwashing is definedherein as the time it takes for the entire washing process; i.e. thetime for the wash cycle(s) and rinse cycle(s) together.

The term “detergent composition”, includes unless otherwise indicated,granular or powder-form all-purpose or heavy-duty washing agents,especially cleaning detergents; liquid, gel or paste-form all-purposewashing agents, especially the so-called heavy-duty liquid (HDL) types;liquid fine-fabric detergents; hand dishwashing agents or light dutydishwashing agents, especially those of the high-foaming type; machinedishwashing agents, including the various tablet, granular, liquid andrinse-aid types for household and institutional use; liquid cleaning anddisinfecting agents, including antibacterial hand-wash types, cleaningbars, soap bars, mouthwashes, denture cleaners, car or carpet shampoos,bathroom cleaners; hair shampoos and hair-rinses; shower gels, foambaths; metal cleaners; as well as cleaning auxiliaries such as bleachadditives and “stain-stick” or pre-treat types. The terms “detergentcomposition” and “detergent formulation” are used in reference tomixtures which are intended for use in a wash medium for the cleaning ofsoiled objects. In some embodiments, the term is used in reference tolaundering fabrics and/or garments (e.g., “laundry detergents”). Inalternative embodiments, the term refers to other detergents, such asthose used to clean dishes, cutlery, etc. (e.g., “dishwashingdetergents”).

The term “automatic dishwashing detergent composition” refers tocompositions comprising detergent components, which composition isintended for cleaning dishware such as plates, cups, glasses, bowls,cutlery such as spoons, knives, forks, serving utensils, ceramics,plastics, metals, china, glass and acrylics in a dishwashing machine. Itis not intended that the present invention be limited to any particulardetergent formulation or composition.

The term “detergent composition” is not intended to be limited tocompositions that contain surfactants. It is intended that in additionto the enzymes herein described, the detergents compositions maycomprise, e.g. one or more additional components selected fromstabilizing agents, surfactants, hydrotopes, builders, co-builders,chelating agents, bleaching systems, bleach activators, bleachcatalysts, polymers, metal care agents, glass care agents, crystalgrowth inhibitors and fabric-hueing agents.

The term “non-fabric detergent compositions” include non-textile surfacedetergent compositions, including but not limited to compositions forhard surface cleaning, such as dishwashing detergent compositions, oraldetergent compositions, denture detergent compositions, and personalcleansing compositions.

The term “effective amount of enzyme” refers to the quantity of enzymenecessary to achieve the enzymatic activity required in the specificapplication, e.g., in a defined detergent composition. Such effectiveamounts are readily ascertained by one of ordinary skill in the art andare based on many factors, such as the particular enzyme used, thecleaning application, the specific composition of the detergentcomposition, and whether a liquid or dry (e.g., granular, bar)composition is required, and the like. The term “effective amount” of anenzyme refers to the quantity of enzyme described hereinbefore thatachieves a desired level of enzymatic activity, e.g., in a defineddetergent composition. In one embodiment, the effective amount of aprotease is the same as the effective amount of an alpha-amylase. Inanother embodiment, the effective amount of a protease is different tothe effective amount of an alpha-amylase, e.g., the effective amount ofa protease may be more or may be less than the effective amount of analpha-amylase.

The term “water hardness” or “degree of hardness” or “dH” or “° dH” asused herein refers to German degrees of hardness. One degree is definedas 10 milligrams of calcium oxide per litre of water.

The term “relevant washing conditions” is used herein to indicate theconditions, particularly washing temperature, time, washing mechanics,detergent concentration, type of detergent and water hardness, actuallyused in households in a detergent market segment.

The term “adjunct materials” means any liquid, solid or gaseous materialselected for the particular type of detergent composition desired andthe form of the product (e.g., liquid, granule, powder, bar, paste,spray, tablet, gel, or foam composition), which materials are alsopreferably compatible with the enzymes used in the composition. In someembodiments, granular compositions are in “compact” form, while in otherembodiments, the liquid compositions are in a “concentrated” form.

The term “stain removing enzyme” as used herein, describes an enzymethat aids the removal of a stain or soil from a fabric or a hardsurface. Stain removing enzymes act on specific substrates, e.g.,protease on protein, amylase on starch, lipase and cutinase on lipids(fats and oils), pectinase on pectin and hemicellulases onhemicellulose. Stains are often depositions of complex mixtures ofdifferent components which either results in a local discolouration ofthe material by itself or which leaves a sticky surface on the objectwhich may attract soils dissolved in the washing liquor therebyresulting in discolouration of the stained area. When an enzyme acts onits specific substrate present in a stain the enzyme degrades orpartially degrades its substrate thereby aiding the removal of soils andstain components associated with the substrate during the washingprocess. For example, when a protease acts on a grass stain it degradesthe protein components in the grass and allows the green/brown colour tobe released during washing.

The term “reduced amount” means in this context that the amount of thecomponent is smaller than the amount which would be used in a referenceprocess under otherwise the same conditions. In a preferred embodimentthe amount is reduced by, e.g., at least 5%, such as at least 10%, atleast 15%, at least 20% or as otherwise herein described.

The term “low detergent concentration” system includes detergents whereless than about 800 ppm of detergent components is present in the washwater. Asian, e.g., Japanese detergents are typically considered lowdetergent concentration systems.

The term “medium detergent concentration” system includes detergentswherein between about 800 ppm and about 2000 ppm of detergent componentsis present in the wash water. North American detergents are generallyconsidered to be medium detergent concentration systems.

The term “high detergent concentration” system includes detergentswherein greater than about 2000 ppm of detergent components is presentin the wash water. European detergents are generally considered to behigh detergent concentration systems.

The term “liquid laundry detergent composition” as used herein refers toa detergent composition which is in a stabilized liquid form and used ina method for laundering a fabric. Thus, the detergent composition hasbeen formulated to be in fluid form.

The term “powder laundry detergent composition” as used herein refers toa detergent composition which is in a solid form, such as a granulate,non-dusting granulate or powder, which is used in a method forlaundering a fabric.

The term “liquid dishwash detergent composition” as used herein refersto a detergent composition which is in a stabilized liquid form and usedin dishwash. Dishwash may be any kind of dishwash, such as manualdishwash and such as automated dishwash (ADW).

The term “powder dishwash detergent composition” as used herein refersto a detergent composition which is in a solid form, such as agranulate, powder or compact unit and used in dishwash. A powderdishwash detergent composition is typically used in automated dishwash,but the used is not limited to such ADW, and may also be intended forused in any other kind of dishwash, such as manual dishwash.

The terms “Delta intensity” or “Delta intensity value” are definedherein as the result of an intensity measurement of a test material,e.g. a Melamine tiles stained with starch DM-277 (Center ForTestmaterials BV, P.O. Box 120, 3133 KT Vlaardingen, the Netherlands) ora hard surface. The delta intensity is the intensity value of the testmaterial washed with amylase subtracting the intensity value of the testmaterial washed without amylase.

The term “numbering is according to” as used herein, refers to the wayeach of the amino acid residues in a polypeptide of the presentinvention is numbered. I.e. the skilled person would know that when,e.g. position 202 is numbered according to SEQ ID NO: 1, he would knowthat by alignment of any other polypeptide with SEQ ID NO: 1, he will beable to determine the corresponding amino acid residue in the otherpolypeptide. Alignment of two or more amino acid sequences has beendescribed elsewhere herein.

Conventions for Designation of Variants

For purposes of the present invention, the polypeptide disclosed in SEQID NO: 1 is used to determine the corresponding amino acid residue inanother alpha-amylase. The amino acid sequence of another alpha-amylaseis aligned with the polypeptide disclosed in SEQ ID NO: 1, and based onthe alignment, the amino acid position number corresponding to any aminoacid residue in the polypeptide disclosed in SEQ ID NO: 1 is determinedusing the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J.Mol. Biol. 48: 443-453) as implemented in the Needle program of theEMBOSS package (EMBOSS: The European Molecular Biology Open SoftwareSuite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version3.0.0 or later.

Identification of the corresponding amino acid residue in anotheralpha-amylase can be confirmed by an alignment of multiple polypeptidesequences using “ClustalW” (Larkin et al., 2007, Bioinformatics 23:2947-2948).

When the other enzyme has diverged from the polypeptide of SEQ ID NO: 1such that traditional sequence-based comparison fails to detect theirrelationship (Lindahl and Elofsson, 2000, J. Mol. Biol. 295: 613-615),other pairwise sequence comparison algorithms can be used. Greatersensitivity in sequence-based searching can be attained using searchprograms that utilize probabilistic representations of polypeptidefamilies (profiles) to search databases. For example, the PSI-BLASTprogram generates profiles through an iterative database search processand is capable of detecting remote homologs (Atschul et al., 1997,Nucleic Acids Res. 25: 3389-3402). Even greater sensitivity can beachieved if the family or superfamily for the polypeptide has one ormore representatives in the protein structure databases. Programs suchas GenTHREADER (Jones, 1999, J. Mol. Biol. 287: 797-815; McGuffin andJones, 2003, Bioinformatics 19: 874-881) utilize information from avariety of sources (PSI-BLAST, secondary structure prediction,structural alignment profiles, and solvation potentials) as input to aneural network that predicts the structural fold for a query sequence.Similarly, the method of Gough et al., 2000, J. Mol. Biol. 313: 903-919,can be used to align a sequence of unknown structure with thesuperfamily models present in the SCOP database. These alignments can inturn be used to generate homology models for the polypeptide, and suchmodels can be assessed for accuracy using a variety of tools developedfor that purpose.

For proteins of known structure, several tools and resources areavailable for retrieving and generating structural alignments. Forexample the SCOP superfamilies of proteins have been structurallyaligned, and those alignments are accessible and downloadable. Two ormore protein structures can be aligned using a variety of algorithmssuch as the distance alignment matrix (Holm and Sander, 1998, Proteins33: 88-96) or combinatorial extension (Shindyalov and Bourne, 1998,Protein Engineering 11: 739-747), and implementation of these algorithmscan additionally be utilized to query structure databases with astructure of interest in order to discover possible structural homologs(e.g., Holm and Park, 2000, Bioinformatics 16: 566-567).

In describing the variants of the present invention, the nomenclaturedescribed below is adapted for ease of reference. The accepted IUPACsingle letter or three letter amino acid abbreviation is employed.

Substitutions: For an amino acid substitution, the followingnomenclature is used: Original amino acid, position, substituted aminoacid. Accordingly, the substitution of threonine at position 226 withalanine is designated as “Thr226Ala” or “T226A”. Multiple mutations areseparated by addition marks (“+”), e.g., “Gly205Arg+Ser411Phe” or“G205R+S411F”, representing substitutions at positions 205 and 411 ofglycine (G) with arginine (R) and serine (S) with phenylalanine (F),respectively.

Deletions: For an amino acid deletion, the following nomenclature isused: Original amino acid, position, *. Accordingly, the deletion ofglycine at position 195 is designated as “Gly195*” or “G195*”. Multipledeletions are separated by addition marks (“+”), e.g., “Gly195*+Ser411*”or “G195*+S411*”.

Insertions. For an amino acid insertion, the following nomenclature isused: Original amino acid, position, original amino acid, inserted aminoacid. Accordingly, the insertion of lysine after glycine at position 195is designated “Gly195GlyLys” or “G195GK”. An insertion of multiple aminoacids is designated [Original amino acid, position, original amino acid,inserted amino acid #1, inserted amino acid #2; etc.]. For example, theinsertion of lysine and alanine after glycine at position 195 isindicated as “Gly195GlyLysAla” or “G195GKA”.

In such cases the inserted amino acid residue(s) are numbered by theaddition of lower case letters to the position number of the amino acidresidue preceding the inserted amino acid residue(s). In the aboveexample, the sequence would thus be:

Parent: Variant: 195 195 195a 195b G G - K -A

Multiple modifications: Variants comprising multiple modifications areseparated by addition marks (“+”), e.g., “Arg170Tyr+Gly195Glu” or“R170Y+G195E” representing a substitution of arginine and glycine atpositions 170 and 195 with tyrosine and glutamic acid, respectively.

Different modifications: Where different modifications may be introducedat a position, the different alterations are separated by a comma, e.g.,“Arg170Tyr,Glu” represents a substitution of arginine at position 170with tyrosine or glutamic acid. Thus, “Tyr167Gly,Ala+Arg170Gly,Ala”designates the following variants:

“Tyr167Gly+Arg170Gly”, “Tyr167Gly+Arg170Ala”, “Tyr167Ala+Arg170Gly”, and“Tyr167Ala+Arg170Ala”. DETAILED DESCRIPTION OF THE INVENTION

The present invention relates an alpha-amylase variant of a parentalpha-amylase comprising a modification in one or more (e.g., several)positions corresponding to position: 1, 2, 7, 9, 11, 16, 19, 25, 37, 43,48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116,118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158,160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182,186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246,256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284,286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315,317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391,395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469,473, 476 and 481, using SEQ ID NO: 1 for numbering and wherein eachmodification is independently a substitution, insertion, or deletion,and wherein said variant has at least 59%, e.g. at least 60%, e.g. atleast 65%, e.g., at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100% sequence identity to the polypeptide ofSEQ ID NOs: 1-17, and wherein said variant has alpha-amylase activityand wherein the said variant has improved wash performance compared tosaid parent polypeptide.

In one aspect, the present invention relates to alpha-amylase variant ofa parent alpha-amylase polypeptide having alpha-amylase activity. Thus,in particular aspect, the present invention relates to variant of aparent alpha-amylase polypeptide having alpha-amylase activity, whereinsaid variant has an improved wash performance, and wherein said varianthas alpha-amylase activity.

In one aspect, the present invention relates to an alpha-amylase variantof a parent alpha-amylase comprising a modification in one or more(e.g., several) positions corresponding to position: 1, 2, 7, 9, 11, 16,19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104,109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139,142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175,176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212,T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273,274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306,310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365,377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445,458, 465, 466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering,and wherein each modification is independently a substitution,insertion, or deletion, and wherein said variant has at least 59%, e.g.at least 60%, e.g. at least 65%, e.g., at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, or at least 99%, but less than 100% sequenceidentity to the polypeptide of SEQ ID NOs: 1-17, and wherein saidvariant has alpha-amylase activity and wherein the said variant hasimproved wash performance compared to said parent polypeptide andwherein said parent alpha-amylase has amino acid sequence of SEQ ID NO:1 or SEQ ID NO: 2.

In one aspect, the number of alterations is 1-50, e.g., 1-45, 1-40,1-35, 1-30, 1-25, 1-20, 1-15, 1-10 or 1-5, such as 1, 2, 3, 4, 5, 6, 7,8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49 or 50 alterations.

In one aspect, the number of substitutions is 1-50, e.g., 1-45, 1-40,1-35, 1-30, 1-25, 1-20, 1-15, 1-10 or 1-5, such as 1, 2, 3, 4, 5, 6, 7,8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49 or 50 substitutions.

In one aspect, the substituted amino acid residue is different from thenaturally-occurring amino acid residue in that position. In oneembodiment, the substitution is selected from the group consisting of A,C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W and Y, with theproviso that the substituted amino acid residue is different from thenaturally-occurring amino acid residue in that position.

In certain embodiments, the variant may comprise a pairwise deletionwithin a particular loop of the alpha-amylase which has been found tofurther stabilize the alpha-amylase. Thus, in one embodiment, thevariant further comprises a pairwise deletion of the amino acid residuescorresponding to R181, G182, D183 and G184, using SEQ ID NO: 1 fornumbering, with the proviso that when the amino acids in positionscorresponding to R181 and/or G184 has been substituted, the pairwisedeletion is in the positions corresponding to G182 and D183, using SEQID NO: 1 for numbering.

The term “pairwise deletion” as used herein, refers to one deletion intwo separate positions. Such positions may be adjacent to one anotherbut are not limited to such adjacent pairs. A pairwise deletion maythus, also be deletion of one amino acid and another amino acid whichmay be up to three amino acids further downstream or upstream from thefirst deletion. Accordingly, in one embodiment, the variant comprises apairwise deletion of the amino acid residues selected from the groupconsisting of; R181+G182, R181+D183, R181+G184, G182+D183, G182+G182,and D183+G184, using SEQ ID NO:1 for numbering.

In one aspect, SEQ ID NO: 2 is the amino acid sequence comprising adouble deletion of the amino acid residues selected from the groupconsisting of; R181+G182, R181+D183, R181+G184, G182+D183, G182+G182,and D183+G184, preferably D183+G184, using SEQ ID NO: 1 for numbering.

In one aspect, the number of deletion is 1-50, e.g., 1-45, 1-40, 1-35,1-30, 1-25, 1-20, 1-15, 1-10 or 1-5, such as 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49 or 50 deletion.

In another aspect, a variant comprises a modification at one or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at two or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at three or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at four or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at five or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at six or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identitysequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at seven or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at eight or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at nine or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at ten or morepositions corresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25,37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111,113, 116, 118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144,149, 158, 160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178,181, 182, 186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235,238, 246, 256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275,276, 284, 286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311,314, 315, 317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375,385, 391, 395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465,466, 469, 473, 476 and 481, using SEQ ID NO: 1 for numbering and whereinsaid variant has alpha-amylase activity and wherein said variant has atleast 60%, at least 65%, at least 70%, at least 75%, at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

In another aspect, a variant comprises a modification at each positionscorresponding to any of positions 1, 2, 7, 9, 11, 16, 19, 25, 37, 43,48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116,118, 125, 127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158,160, 163, 167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182,186, 187, 195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246,256, 259, 264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284,286, 291, 293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315,317, 319, 320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391,395, 400, 406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469,473, 476 and 481, using SEQ ID NO: 1 for numbering and wherein saidvariant has alpha-amylase activity and wherein said variant has at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 95%, at least 96%, at least 97%, at least98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

In one aspect, a variant useful herein comprises one or more of thefollowing modification at position corresponding to positions 1, 2, 7,9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90,98, 104, 109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134, 135,136, 139, 142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172, 173,174, 175, 176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206, 209,210, 212, T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269, 270,272, 273, 274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302, 303,304, 306, 310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339, 345,357, 365, 377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435, 439,444, 445, 458, 465, 466, 469, 473, 476 and 481, using SEQ ID NO: 1 fornumbering and wherein said variant has alpha-amylase activity andwherein said variant has at least 60%, at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, or at least 99%, but less than100% sequence identity to the polypeptide of SEQ ID NOs: 1-17.

In one aspect, the variant comprises one or more of the followingmodification at position corresponding to positions: H1, H2, G7, I9,Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81,E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481, using SEQ ID NO: 1 for numbering and wherein said varianthas at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, or at least 99%, but lessthan 100% sequence identity to the polypeptide of SEQ ID NOs: 1-17.

In another aspect, the variant comprises or consists of a deletion at aposition corresponding to position 1. In another aspect, the amino acidat a position corresponding to position 1 is deleted of the polypeptideof SEQ ID NO: 2. In another aspect, the variant comprises or consists ofdeletion H1* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion at aposition corresponding to position 2. In another aspect, the amino acidat a position corresponding to position 2 is deleted of the polypeptideof SEQ ID NO: 2. In another aspect, the variant comprises or consists ofdeletion H2* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 7. In another aspect, the aminoacid at a position corresponding to position 7 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution G7A of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 9. In another aspect, the aminoacid at a position corresponding to position 9 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution I9M of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 11. In another aspect, the aminoacid at a position corresponding to position 11 is substituted with Ala,Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution Q11H of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 16. In another aspect, the aminoacid at a position corresponding to position 16 is substituted with Ala,Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution N16E or N16H or N16Y of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 19. In another aspect, the aminoacid at a position corresponding to position 19 is substituted with Ala,Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution N19D of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 25. In another aspect, the aminoacid at a position corresponding to position 25 is substituted with Ala,Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution N25K or N25M or N25T of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 37. In another aspect, the aminoacid at a position corresponding to position 37 is substituted with Arg,Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution A37V of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 43. In another aspect, the aminoacid at a position corresponding to position 43 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution W43Y of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 48. In another aspect, the aminoacid at a position corresponding to position 48 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution W48Y of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 54. In another aspect, the aminoacid at a position corresponding to position 54 is substituted with Ala,Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution N54Q or N54S of the polypeptide of SEQ IDNO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 56. In another aspect, the aminoacid at a position corresponding to position 56 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, or Tyr. In another aspect, the variant comprises orconsists of the substitution V56I or V56T of the polypeptide of SEQ IDNO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 58. In another aspect, the aminoacid at a position corresponding to position 58 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, or Val. In another aspect, the variant comprises orconsists of the substitution Y58F of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 59. In another aspect, the aminoacid at a position corresponding to position 59 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution G59A of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 60. In another aspect, the aminoacid at a position corresponding to position 60 is substituted with Arg,Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution A60S or A60T of the polypeptide of SEQ IDNO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 63. In another aspect, the aminoacid at a position corresponding to position 63 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution L63F of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 81. In another aspect, the aminoacid at a position corresponding to position 81 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution T81A of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 84. In another aspect, the aminoacid at a position corresponding to position 84 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution E84Q of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 86. In another aspect, the aminoacid at a position corresponding to position 86 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution E86L of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 90. In another aspect, the aminoacid at a position corresponding to position 90 is substituted with Ala,Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution R90H or R90N or R90Q of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 98. In another aspect, the aminoacid at a position corresponding to position 98 is substituted with Ala,Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser,Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution Q98N of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 104. In another aspect, theamino acid at a position corresponding to position 104 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp or Tyr. In another aspect, the variant comprisesor consists of the substitution V104A of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 109. In another aspect, theamino acid at a position corresponding to position 109 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G109A of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 111. In another aspect, theamino acid at a position corresponding to position 111 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A111T of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 113. In another aspect, theamino acid at a position corresponding to position 113 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F113A or F113G or F113N orF113Q or F113T or F113Y of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 116. In another aspect, theamino acid at a position corresponding to position 116 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R116C or R116D or R116F orR116H or R116K or R116N or R116S or R116Y of the polypeptide of SEQ IDNO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 118. In another aspect, theamino acid at a position corresponding to position 118 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q118N or Q118T of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 125. In another aspect, theamino acid at a position corresponding to position 125 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q125G or Q125S of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 127. In another aspect, theamino acid at a position corresponding to position 127 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R127A or R127F or R127H orR127L or R127N or R127T of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 130. In another aspect, theamino acid at a position corresponding to position 130 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution E130D or E130F or E130I orE130K or E130L or E130N or E130S or E130T or E130V of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 132. In another aspect, theamino acid at a position corresponding to position 132 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S132A or S132D or S132F orS132L or S132M or S132P or S132T of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 133. In another aspect, theamino acid at a position corresponding to position 133 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G133D of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 134. In another aspect, theamino acid at a position corresponding to position 134 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T134C or T134E or T134N orT134P or T134R or T134S or T134W or T134Y or T134A of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 135. In another aspect, theamino acid at a position corresponding to position 135 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y135H of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 136. In another aspect, theamino acid at a position corresponding to position 136 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q136D or Q136E or Q136G or136N or Q136R or Q136S of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 139. In another aspect, theamino acid at a position corresponding to position 139 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A139T of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 142. In another aspect, theamino acid at a position corresponding to position 142 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G142H of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 144. In another aspect, theamino acid at a position corresponding to position 144 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N144H of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 149. In another aspect, theamino acid at a position corresponding to position 149 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G149A of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 158. In another aspect, theamino acid at a position corresponding to position 158 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R158C or R158H or R158K orR158Q or R158S of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 160. In another aspect, theamino acid at a position corresponding to position 160 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y160D or Y160F or Y160H orY160N of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 163. In another aspect, theamino acid at a position corresponding to position 163 is substitutedwith Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D163H of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion at aposition corresponding to position 167. In another aspect, the variantcomprises or consists of the deletion W167* of the polypeptide of SEQ IDNO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 169. In anotheraspect, the amino acid at a position corresponding to position 169 issubstituted with Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the deletion Q169* of the polypeptideof SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 170. In anotheraspect, the amino acid at a position corresponding to position 170 issubstituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu,Lys, Met, Phe, Pro, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution 5170A of thepolypeptide of SEQ ID NO: 2. In another aspect, the variant comprises orconsists of deletion S170* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 171. In anotheraspect, the amino acid at a position corresponding to position 171 issubstituted with Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution R171H or R171K orR171L or R171Y of the polypeptide of SEQ ID NO: 2. In another aspect,the variant comprises or consists of deletion R171* of the polypeptideof SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 172. In anotheraspect, the amino acid at a position corresponding to position 172 issubstituted with Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution Q172K or Q172N orQ172S of the polypeptide of SEQ ID NO: 2. In another aspect, the variantcomprises or consists of deletion Q172* of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 173. In anotheraspect, the amino acid at a position corresponding to position 173 issubstituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution L173K of thepolypeptide of SEQ ID NO: 2. In another aspect, the variant comprises orconsists of deletion L173* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 174. In anotheraspect, the amino acid at a position corresponding to position 174 issubstituted with Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution A174N or A174S of thepolypeptide of SEQ ID NO: 2. In another aspect, the variant comprises orconsists of deletion A174* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 175. In anotheraspect, the amino acid at a position corresponding to position 175 issubstituted with Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution N175S of thepolypeptide of SEQ ID NO: 2. In another aspect, the variant comprises orconsists of deletion N175* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 176. In anotheraspect, the amino acid at a position corresponding to position 176 issubstituted with Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys,Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, thevariant comprises or consists of the substitution R176A of thepolypeptide of SEQ ID NO: 2. In another aspect, the variant comprises orconsists of deletion R176* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 178. In another aspect, theamino acid at a position corresponding to position 178 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y178F of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 181. In anotheraspect, the amino acid at a position corresponding to position 181 issubstituted with Ala, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R181A or R181C or R181E orR181G or R181H or R181K or R181N or R181Q of the polypeptide of SEQ IDNO: 2. In another aspect, the variant comprises or consists of deletionR181* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a deletion orsubstitution at a position corresponding to position 182. In anotheraspect, the amino acid at a position corresponding to position 182 issubstituted with Ala, Arg, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G182T N175S of the polypeptideof SEQ ID NO: 2. In another aspect, the variant comprises or consists ofdeletion G182* of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 186. In another aspect, theamino acid at a position corresponding to position 186 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution A186D or A186E or A186H or A186K orA186N or A186T of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 187. In another aspect, theamino acid at a position corresponding to position 187 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution W187M of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 195. In another aspect, theamino acid at a position corresponding to position 195 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N195D or N195F or N195H or N195Q orN195Y of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 202. In another aspect, theamino acid at a position corresponding to position 202 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution M202L of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 203. In another aspect, theamino acid at a position corresponding to position 203 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y203L of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 204. In another aspect, theamino acid at a position corresponding to position 204 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution A204V of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 206. In another aspect, theamino acid at a position corresponding to position 206 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V206L of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 209. In another aspect, theamino acid at a position corresponding to position 209 is substitutedwith Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution D209N of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 210. In another aspect, theamino acid at a position corresponding to position 210 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution H210K or H210N of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 212. In another aspect, theamino acid at a position corresponding to position 212 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution E212D of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 227. In another aspect, theamino acid at a position corresponding to position 227 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution T227K or T227N of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 235. In another aspect, theamino acid at a position corresponding to position 235 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L235I of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 238. In another aspect, theamino acid at a position corresponding to position 238 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V238A of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 246. In another aspect, theamino acid at a position corresponding to position 246 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution M246I or M246L or M246T or M246V of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 256. In another aspect, theamino acid at a position corresponding to position 256 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q256H or Q256N of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 259. In another aspect, theamino acid at a position corresponding to position 259 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution K259G or K259H of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 264. In another aspect, theamino acid at a position corresponding to position 264 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V264A or V246I or V264T of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 265. In another aspect, theamino acid at a position corresponding to position 265 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution A265G of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 266. In another aspect, theamino acid at a position corresponding to position 266 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution E266V of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 267. In another aspect, theamino acid at a position corresponding to position 267 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y267F or Y267H or Y267L of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 269. In another aspect, theamino acid at a position corresponding to position 269 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution K269M or K269Q or K269R of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 270. In another aspect, theamino acid at a position corresponding to position 270 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N270G or N270P of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 272. In another aspect, theamino acid at a position corresponding to position 272 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L272I of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 273. In another aspect, theamino acid at a position corresponding to position 273 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution G273V of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 274. In another aspect, theamino acid at a position corresponding to position 274 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution A274K or A274S of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 275. In another aspect, theamino acid at a position corresponding to position 275 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L275A or L275I or L275V of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 276. In another aspect, theamino acid at a position corresponding to position 276 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution E276N of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 291. In another aspect, theamino acid at a position corresponding to position 291 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variantcomprises or consists of the substitution V291A or V291I or V291T of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 284. In another aspect, theamino acid at a position corresponding to position 284 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution W284R or W284Y of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 286. In another aspect, theamino acid at a position corresponding to position 286 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution M286L of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 291. In another aspect, theamino acid at a position corresponding to position 291 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V291A or V291I or V291T of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 293. In another aspect, theamino acid at a position corresponding to position 293 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L293I or L293V of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 295. In another aspect, theamino acid at a position corresponding to position 295 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y295F of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 298. In another aspect, theamino acid at a position corresponding to position 298 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y298E or Y298N of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 299. In another aspect, theamino acid at a position corresponding to position 299 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q299N or Q299Y of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 302. In another aspect, theamino acid at a position corresponding to position 302 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N302A or N302H or N302K or N302Q of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 303. In another aspect, theamino acid at a position corresponding to position 303 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution 5303G of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 304. In another aspect, theamino acid at a position corresponding to position 304 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution 5304G or 5304Q of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 306. In another aspect, theamino acid at a position corresponding to position 306 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N306H or N306K or N306Q or N306R orN306Y of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 310. In another aspect, theamino acid at a position corresponding to position 310 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution R310N or R310S or R310Q of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 311. In another aspect, theamino acid at a position corresponding to position 311 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N311K or N311R of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 314. In another aspect, theamino acid at a position corresponding to position 314 is substitutedwith Ala, Arg, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution N314Q of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 315. In another aspect, theamino acid at a position corresponding to position 315 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution G315N of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 317. In another aspect, theamino acid at a position corresponding to position 317 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L317V of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 319. In another aspect, theamino acid at a position corresponding to position 319 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q319H of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 320. In another aspect, theamino acid at a position corresponding to position 320 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution R320K or R320Q or R320S of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 323. In another aspect, theamino acid at a position corresponding to position 323 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution S323T or S323K of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 328. In another aspect, theamino acid at a position corresponding to position 328 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution F328L of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 337. In another aspect, theamino acid at a position corresponding to position 337 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution G337D or G337E of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 339. In another aspect, theamino acid at a position corresponding to position 339 is substitutedwith Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution A339S of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 345. In another aspect, theamino acid at a position corresponding to position 345 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q345N or Q345R of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 357. In another aspect, theamino acid at a position corresponding to position 357 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution L357F of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 365. In another aspect, theamino acid at a position corresponding to position 365 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q365A or Q365C or Q365E or Q365H orQ365K or Q365M or Q365S of the polypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 377. In another aspect, theamino acid at a position corresponding to position 377 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution P377K or P377T of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 375. In another aspect, theamino acid at a position corresponding to position 375 is substitutedwith Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution D375H or D375N or D375Y of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 386. In another aspect, theamino acid at a position corresponding to position 386 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q386L of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 391. In another aspect, theamino acid at a position corresponding to position 391 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution K391A of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 395. In another aspect, theamino acid at a position corresponding to position 395 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q395K of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 400. In another aspect, theamino acid at a position corresponding to position 400 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution R400S of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 406. In another aspect, theamino acid at a position corresponding to position 406 is substitutedwith Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution D406H of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 408. In another aspect, theamino acid at a position corresponding to position 408 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution W408H of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 410. In another aspect, theamino acid at a position corresponding to position 410 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V410I of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 431. In another aspect, theamino acid at a position corresponding to position 431 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution S431F of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 435. In another aspect, theamino acid at a position corresponding to position 435 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution G435C of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 439. In another aspect, theamino acid at a position corresponding to position 439 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution W439R or W439T of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 444. In another aspect, theamino acid at a position corresponding to position 444 is substitutedwith Ala, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution R444T of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 445. In another aspect, theamino acid at a position corresponding to position 445 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q445S of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 178. In another aspect, theamino acid at a position corresponding to position 178 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Val. In another aspect, the variantcomprises or consists of the substitution Y178F of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 458. In another aspect, theamino acid at a position corresponding to position 458 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variantcomprises or consists of the substitution R458K of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 465. In another aspect, theamino acid at a position corresponding to position 465 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variantcomprises or consists of the substitution N465G of the polypeptide ofSEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 466. In another aspect, theamino acid at a position corresponding to position 466 is substitutedwith Ala, Arg, Asn, Asp, Cys, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Q466S of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 469. In another aspect, theamino acid at a position corresponding to position 469 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution W469N or W469Y of the polypeptide of SEQID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 178. In another aspect, theamino acid at a position corresponding to position 178 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution Y178F of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 473. In another aspect, theamino acid at a position corresponding to position 473 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variantcomprises or consists of the substitution F473I or F473P or F473S of thepolypeptide of SEQ ID NO: 2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 476. In another aspect, theamino acid at a position corresponding to position 476 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr or Val. In another aspect, the variant comprisesor consists of the substitution G476K of the polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of a substitutionat a position corresponding to position 481. In another aspect, theamino acid at a position corresponding to position 481 is substitutedwith Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met,Phe, Pro, Ser, Thr, Trp, or Tyr. In another aspect, the variantcomprises or consists of the substitution V481A of the polypeptide ofSEQ ID NO: 2.

In one aspect, the variant comprises one or more of the followingmodification at position corresponding to positions: H1*; H2*, G7A; I9M;Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y; N54Q;N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L; R90H;R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering, and wherein said variant has at least 59%, e.g. at least60%, e.g. at least 65%, e.g., at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17.

The variants have at least 20%, e.g., at least 40%, at least 50%, atleast 60%, at least 70%, at least 80%, at least 90%, at least 95%, or atleast 100% of the alpha-amylase activity of the polypeptide of SEQ IDNOs: 1-17.

In an embodiment, the variant has sequence identity of at least 60%, atleast 65%, at least 70%, at least 75%, at least 80%, at least 85%, atleast 90%, at least 91%, at least 92%, at least 93%, at least 94%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100%, to the amino acid sequence of the parent polypeptidehaving alpha-amylase activity.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 1.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 2.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 3.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 4.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 5.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 6.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 7.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 8.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 9.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 10.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 11.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 12.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 13.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 14.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 15.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 16.

In another embodiment, the variant has sequence identity of at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 91%, at least 92%, at least 93%, at least94%, at least 95%, such as at least 96%, at least 97%, at least 98%, orat least 99%, but less than 100%, sequence identity to SEQ ID NO: 17.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 1.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 2.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 4.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 5.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; 5170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 6.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 7.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 8.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 9.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:10.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:11.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:12.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:13.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:14.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; 5170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:15.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:16.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: H1*; H2*, G7A;I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M; N25T; A37V; W43Y; W48Y;N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T; L63F; T81A; E84Q; E86L;R90H; R90N; R90Q; Q98N; V104A; G109A; A111T; F113A; F113G; F113N; F113Q;F113T; F113Y; R116C; R116D; R116F; R116H; R116K; R116N; R116S; R116Y;Q118N; Q118T; Q125G; Q125S; R127A; R127F; R127H; R127L; R127N; R127T;E130D; E130F; E130I; E130K; E130L; E130N; E130S; E130T; E130V; S132A;S132D; S132F; S132L; S132M; S132P; S132T; G133D; T134C; T134E; T134N;T134P; T134R; T134S; T134W; T134Y; T134A; Y135H; Q136D; Q136E; Q136G;Q136N; Q136R; Q136S; A139T; G142H; N144H; G149A; R158C; R158H; R158K;R158Q; R158S; Y160D; Y160F; T160H; Y160N; D163H; W167*; Q169*; Q169E;Q169F; S170*; 5170A; R171*; R171H; R171K; R171L; R171Y; Q172*; Q172K;Q172N; Q172S; L173*; L173K; A174*; A174N; A174S; N175*; N175S; R176*;R176A; Y178F; R181*; R181A; R181C; R181E; R181G; R181H; R181K; R181N;R181Q; G182*; G182T; A186D; A186E; A186H; A186K; A186N; A186T; W187M;N195D; N195F; N195H; N195Q; N195Y; M202L; Y203L; A204V; V206L; D209N;H210K; H210N; E212D; T227K; T227N; L235I; V238A; M246I; M246L; M246T;M246V; Q256H; Q256N; K259G; K259H; V264A; V246I; V264T; A265G; E266V;Y267F; Y267H; Y267L; K269M; K269Q; K269R; N270G; N270P; L272I; G273V;A274K; A274S; L275A; L275I; L275V; E276N; W284R; W284Y; M286L; V291A;V291I; V291T; L293I; L293V; Y295F; Y298E; Y298N; Q299N; Q299Y; N302A;N302H; N302K; N302Q; 5303G; 5304G; 5304Q; N306H; N306K; N306Q; N306R;N306Y; R310N; R310S; R310Q; N311K; N311R; N314Q; G315N; L317V; Q319H;R320K; R320Q; R320S; S323T; H323K; F328L; G337D; G337E; A339S; Q345N;Q345R; L357F; Q365A; Q365C; Q365E; Q365H; Q365K; Q365M; Q365S; P377K;P377T; D375H; D375N; D375Y; Q386L; K391A; Q395K; R400S; D406H; W408H;V410I; S431F; G435C; W439R; W439T; R444T; Q445S; R458K; N465G; Q466S;W469N; W469Y; F473I; F473P; F473S; G476K; and V481A, using SEQ ID NO: 1for numbering and wherein said variant has at least 60%, at least 65%,at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:17.

In one aspect, the modification in one or more positions correspond tothe following: H1*; H2*, G7A; I9M; Q11H; N16E; N16H; N16Y; N19D; N25K;N25M; N25T; A37V; W43Y; W48Y; N54Q; N54S; V56I; V56T; Y58F; G59A; A60S;A60T; L63F; T81A; E84Q; E86L; R90H; R90N; R90Q; Q98N; V104A; G109A;A111T; F113A; F113G; F113N; F113Q; F113T; F113Y; R116C; R116D; R116F;R116H; R116K; R116N; R116S; R116Y; Q118N; Q118T; Q125G; Q125S; R127A;R127F; R127H; R127L; R127N; R127T; E130D; E130F; E130I; E130K; E130L;E130N; E130S; E130T; E130V; S132A; S132D; S132F; S132L; S132M; S132P;S132T; G133D; T134C; T134E; T134N; T134P; T134R; T134S; T134W; T134Y;T134A; Y135H; Q136D; Q136E; Q136G; Q136N; Q136R; Q136S; A139T; G142H;N144H; G149A; R158C; R158H; R158K; R158Q; R158S; Y160D; Y160F; T160H;Y160N; D163H; W167*; Q169*; Q169E; Q169F; S170*; 5170A; R171*; R171H;R171K; R171L; R171Y; Q172*; Q172K; Q172N; Q172S; L173*; L173K; A174*;A174N; A174S; N175*; N175S; R176*; R176A; Y178F; R181*; R181A; R181C;R181E; R181G; R181H; R181K; R181N; R181Q; G182*; G182T; A186D; A186E;A186H; A186K; A186N; A186T; W187M; N195D; N195F; N195H; N195Q; N195Y;M202L; Y203L; A204V; V206L; D209N; H210K; H210N; E212D; T227K; T227N;L235I; V238A; M246I; M246L; M246T; M246V; Q256H; Q256N; K259G; K259H;V264A; V246I; V264T; A265G; E266V; Y267F; Y267H; Y267L; K269M; K269Q;K269R; N270G; N270P; L272I; G273V; A274K; A274S; L275A; L275I; L275V;E276N; W284R; W284Y; M286L; V291A; V291I; V291T; L293I; L293V; Y295F;Y298E; Y298N; Q299N; Q299Y; N302A; N302H; N302K; N302Q; 5303G; 5304G;5304Q; N306H; N306K; N306Q; N306R; N306Y; R310N; R310S; R310Q; N311K;N311R; N314Q; G315N; L317V; Q319H; R320K; R320Q; R320S; S323T; H323K;F328L; G337D; G337E; A339S; Q345N; Q345R; L357F; Q365A; Q365C; Q365E;Q365H; Q365K; Q365M; Q365S; P377K; P377T; D375H; D375N; D375Y; Q386L;K391A; Q395K; R400S; D406H; W408H; V410I; S431F; G435C; W439R; W439T;R444T; Q445S; R458K; N465G; Q466S; W469N; W469Y; F473I; F473P; F473S;G476K; and V481A, using SEQ ID NO: 1 for numbering.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions: A174*+A339S,A174*+G109A, A174*+G149A, A174*+G182T, A174*+H1*, A174*+I9M,A174*+K391A, A174*+M202L, A174*+N16Y, A174*+N195F, A174*+N54S,A174*+Q169E, A174*+Q169E, A174*+Q172K, A174*+Q299Y, A174*+Q345R,A174*+Q365S, A174*+R320K, A174*+R458K, A174*+S323T, A174*+V206L,A174*+Y295F, A339S+G109A, A339S+G149A, A339S+G182T, A339S+H1*,A339S+I9M, A339S+K391A, A339S+M202L, A339S+N16Y, A339S+N195F,A339S+N54S, A339S+Q169E, A339S+Q169E, A339S+Q172K, A339S+Q299Y,A339S+Q345R, A339S+Q365S, A339S+R320K, A339S+R458K, A339S+S323T,A339S+V206L, A339S+Y295F, G109A+G149A, G109A+G182T, G109A+H1*,G109A+I9M, G109A+K391A, G109A+M202L, G109A+N16Y, G109A+N195F,G109A+N54S, G109A+Q169E, G109A+Q169E, G109A+Q172K, G109A+Q299Y,G109A+Q345R, G109A+Q365S, G109A+R320K, G109A+R458K, G109A+S323T,G109A+V206L, G109A+Y295F, G149A+G182T, G149A+H1*, G149A+I9M,G149A+K391A, G149A+M202L, G149A+N16Y, G149A+N195F, G149A+N54S,G149A+Q169E, G149A+Q169E, G149A+Q172K, G149A+Q299Y, G149A+Q345R,G149A+Q365S, G149A+R320K, G149A+R458K, G149A+S323T, G149A+V206L,G149A+Y295F, G182T+H1*, G182T+I9M, G182T+K391A, G182T+M202L, G182T+N16Y,G182T+N195F, G182T+N54S, G182T+Q169E, G182T+Q169E, G182T+Q172K,G182T+Q299Y, G182T+Q345R, G182T+Q365S, G182T+R320K, G182T+R458K,G182T+S323T, G182T+V206L, G182T+Y295F, H1*+I9M, H1*+K391A, H1*+M202L,H1*+N16Y, H1*+N195F, H1*+N54S, H1*+Q169E, H1*+Q169E, H1*+Q172K,H1*+Q299Y, H1*+Q345R, H1*+Q365S, H1*+R320K, H1*+R458K, H1*+S323T,H1*+V206L, H1*+Y295F, I9M+K391A, I9M+M202L, I9M+N16Y, I9M+N195F,I9M+N54S, I9M+Q169E, I9M+Q169E, I9M+Q172K, I9M+Q299Y, I9M+Q345R,I9M+Q365S, I9M+R320K, I9M+R458K, I9M+S323T, I9M+V206L, I9M+Y295F,K391A+M202L, K391A+N16Y, K391A+N195F, K391A+N54S, K391A+Q169E,K391A+Q169E, K391A+Q172K, K391A+Q299Y, K391A+Q345R, K391A+Q365S,K391A+R320K, K391A+R458K, K391A+S323T, K391A+V206L, K391A+Y295F,M202L+N16Y, M202L+N195F, M202L+N54S, M202L+Q169E, M202L+Q169E,M202L+Q172K, M202L+Q299Y, M202L+Q345R, M202L+Q365S, M202L+R320K,M202L+R458K, M202L+S323T, M202L+V206L, M202L+Y295F, N16Y+N195F,N16Y+N54S, N16Y+Q169E, N16Y+Q169E, N16Y+Q172K, N16Y+Q299Y, N16Y+Q345R,N16Y+Q365S, N16Y+R320K, N16Y+R458K, N16Y+S323T, N16Y+V206L, N16Y+Y295F,N195F+N54S, N195F+Q169E, N195F+Q169E, N195F+Q172K, N195F+Q299Y,N195F+Q345R, N195F+Q365S, N195F+R320K, N195F+R458K, N195F+S323T,N195F+V206L, N195F+Y295F, N54S+Q169E, N54S+Q169E, N54S+Q172K,N54S+Q299Y, N54S+Q345R, N54S+Q365S, N54S+R320K, N54S+R458K, N54S+S323T,N54S+V206L, N54S+Y295F, Q169E+Q169E, Q169E+Q172K, Q169E+Q299Y,Q169E+Q345R, Q169E+Q365S, Q169E+R320K, Q169E+R458K, Q169E+S323T,Q169E+V206L, Q169E+Y295F, Q169E+Q172K, Q169E+Q299Y, Q169E+Q345R,Q169E+Q365S, Q169E+R320K, Q169E+R458K, Q169E+S323T, Q169E+V206L,Q169E+Y295F, Q172K+Q299Y, Q172K+Q345R, Q172K+Q365S, Q172K+R320K,Q172K+R458K, Q172K+S323T, Q172K+V206L, Q172K+Y295F, Q299Y+Q345R,Q299Y+Q365S, Q299Y+R320K, Q299Y+R458K, Q299Y+S323T, Q299Y+V206L,Q299Y+Y295F, Q345R+Q365S, Q345R+R320K, Q345R+R458K, Q345R+S323T,Q345R+V206L, Q345R+Y295F, Q365S+R320K, Q365S+R458K, Q365S+S323T,Q365S+V206L, Q365S+Y295F, R320K+R458K, R320K+S323T, R320K+V206L,R320K+Y295F, R458K+S323T, R458K+V206L, R458K+Y295F, S323T+V206L,S323T+Y295F, V206L+Y295F, using SEQ ID NO: 1 for numbering and whereinsaid variant has at least 60%, at least 65%, at least 70%, at least 75%,at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, or at least 99%, but less than 100% sequenceidentity to the polypeptide of SEQ ID Nos; 1-17.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions:A174*+A339S+G109A, A174*+A339S+G149A, A174*+A339S+G182T,A174*+A339S+H1*, A174*+A339S+I9M, A174*+A339S+K391A, A174*+A339S+M202L,A174*+A339S+N16Y, A174*+A339S+N195F, A174*+A339S+N54S,A174*+A339S+Q169E, A174*+A339S+Q169E, A174*+A339S+Q172K,A174*+A339S+Q299Y, A174*+A339S+Q345R, A174*+A339S+Q365S,A174*+A339S+R320K, A174*+A339S+R458K, A174*+A339S+S323T,A174*+A339S+V206L, A174*+A339S+Y295F, A174*+G109A+G149A,A174*+G109A+G182T, A174*+G109A+H1*, A174*+G109A+I9M, A174*+G109A+K391A,A174*+G109A+M202L, A174*+G109A+N16Y, A174*+G109A+N195F,A174*+G109A+N54S, A174*+G109A+Q169E, A174*+G109A+Q169E,A174*+G109A+Q172K, A174*+G109A+Q299Y, A174*+G109A+Q345R,A174*+G109A+Q365S, A174*+G109A+R320K, A174*+G109A+R458K,A174*+G109A+S323T, A174*+G109A+V206L, A174*+G109A+Y295F,A174*+G149A+G182T, A174*+G149A+H1*, A174*+G149A+I9M, A174*+G149A+K391A,A174*+G149A+M202L, A174*+G149A+N16Y, A174*+G149A+N195F,A174*+G149A+N54S, A174*+G149A+Q169E, A174*+G149A+Q169E,A174*+G149A+Q172K, A174*+G149A+Q299Y, A174*+G149A+Q345R,A174*+G149A+Q365S, A174*+G149A+R320K, A174*+G149A+R458K,A174*+G149A+S323T, A174*+G149A+V206L, A174*+G149A+Y295F,A174*+G182T+H1*, A174*+G182T+I9M, A174*+G182T+K391A, A174*+G182T+M202L,A174*+G182T+N16Y, A174*+G182T+N195F, A174*+G182T+N54S,A174*+G182T+Q169E, A174*+G182T+Q169E, A174*+G182T+Q172K,A174*+G182T+Q299Y, A174*+G182T+Q345R, A174*+G182T+Q365S,A174*+G182T+R320K, A174*+G182T+R458K, A174*+G182T+S323T,A174*+G182T+V206L, A174*+G182T+Y295F, A174*+H1*+I9M, A174*+H1*+K391A,A174*+H1*+M202L, A174*+H1*+N16Y, A174*+H1*+N195F, A174*+H1*+N54S,A174*+H1*+Q169E, A174*+H1*+Q169E, A174*+H1*+Q172K, A174*+H1*+Q299Y,A174*+H1*+Q345R, A174*+H1*+Q365S, A174*+H1*+R320K, A174*+H1*+R458K,A174*+H1*+S323T, A174*+H1*+V206L, A174*+H1*+Y295F, A174*+I9M+K391A,A174*+I9M+M202L, A174*+I9M+N16Y, A174*+I9M+N195F, A174*+I9M+N54S,A174*+I9M+Q169E, A174*+I9M+Q169E, A174*+I9M+Q172K, A174*+I9M+Q299Y,A174*+I9M+Q345R, A174*+I9M+Q365S, A174*+I9M+R320K, A174*+I9M+R458K,A174*+I9M+S323T, A174*+I9M+V206L, A174*+I9M+Y295F, A174*+K391A+M202L,A174*+K391A+N16Y, A174*+K391A+N195F, A174*+K391A+N54S,A174*+K391A+Q169E, A174*+K391A+Q169E, A174*+K391A+Q172K,A174*+K391A+Q299Y, A174*+K391A+Q345R, A174*+K391A+Q365S,A174*+K391A+R320K, A174*+K391A+R458K, A174*+K391A+S323T,A174*+K391A+V206L, A174*+K391A+Y295F, A174*+M202L+N16Y,A174*+M202L+N195F, A174*+M202L+N54S, A174*+M202L+Q169E,A174*+M202L+Q169E, A174*+M202L+Q172K, A174*+M202L+Q299Y,A174*+M202L+Q345R, A174*+M202L+Q365S, A174*+M202L+R320K,A174*+M202L+R458K, A174*+M202L+S323T, A174*+M202L+V206L,A174*+M202L+Y295F, A174*+N16Y+N195F, A174*+N16Y+N54S, A174*+N16Y+Q169E,A174*+N16Y+Q169E, A174*+N16Y+Q172K, A174*+N16Y+Q299Y, A174*+N16Y+Q345R,A174*+N16Y+Q365S, A174*+N16Y+R320K, A174*+N16Y+R458K, A174*+N16Y+S323T,A174*+N16Y+V206L, A174*+N16Y+Y295F, A174*+N195F+N54S, A174*+N195F+Q169E,A174*+N195F+Q169E, A174*+N195F+Q172K, A174*+N195F+Q299Y,A174*+N195F+Q345R, A174*+N195F+Q365S, A174*+N195F+R320K,A174*+N195F+R458K, A174*+N195F+S323T, A174*+N195F+V206L,A174*+N195F+Y295F, A174*+N54S+Q169E, A174*+N54S+Q169E, A174*+N54S+Q172K,A174*+N54S+Q299Y, A174*+N54S+Q345R, A174*+N54S+Q365S, A174*+N54S+R320K,A174*+N54S+R458K, A174*+N54S+S323T, A174*+N54S+V206L, A174*+N54S+Y295F,A174*+Q169E+Q169E, A174*+Q169E+Q172K, A174*+Q169E+Q299Y,A174*+Q169E+Q345R, A174*+Q169E+Q365S, A174*+Q169E+R320K,A174*+Q169E+R458K, A174*+Q169E+S323T, A174*+Q169E+V206L,A174*+Q169E+Y295F, A174*+Q169E+Q172K, A174*+Q169E+Q299Y,A174*+Q169E+Q345R, A174*+Q169E+Q365S, A174*+Q169E+R320K,A174*+Q169E+R458K, A174*+Q169E+S323T, A174*+Q169E+V206L,A174*+Q169E+Y295F, A174*+Q172K+Q299Y, A174*+Q172K+Q345R,A174*+Q172K+Q365S, A174*+Q172K+R320K, A174*+Q172K+R458K,A174*+Q172K+S323T, A174*+Q172K+V206L, A174*+Q172K+Y295F,A174*+Q299Y+Q345R, A174*+Q299Y+Q365S, A174*+Q299Y+R320K,A174*+Q299Y+R458K, A174*+Q299Y+S323T, A174*+Q299Y+V206 L,A174*+Q299Y+Y295F, A174*+Q345R+Q365S, A174*+Q345R+R320K,A174*+Q345R+R458K, A174*+Q345R+S323T, A174*+Q345R+V206L,A174*+Q345R+Y295F, A174*+Q365S+R320K, A174*+Q365S+R458K,A174*+Q365S+S323T, A174*+Q365S+V206L, A174*+Q365S+Y295F,A174*+R320K+R458K, A174*+R320K+S323T, A174*+R320K+V206L,A174*+R320K+Y295F, A174*+R458K+S323T, A174*+R458K+V206L,A174*+R458K+Y295F, A174*+S323T+V206L, A174*+S323T+Y295F,A174*+V206L+Y295F, A339S+G109A+G149A, A339S+G109A+G182T,A339S+G109A+H1*, A339S+G109A+I9M, A339S+G109A+K391A, A339S+G109A+M202L,A339S+G109A+N16Y, A339S+G109A+N195F, A339S+G109A+N54S,A339S+G109A+Q169E, A339S+G109A+Q169E, A339S+G109A+Q172K,A339S+G109A+Q299Y, A339S+G109A+Q345R, A339S+G109A+Q365S,A339S+G109A+R320K, A339S+G109A+R458K, A339S+G109A+S323T,A339S+G109A+V206L, A339S+G109A+Y295F, A339S+G149A+G182T,A339S+G149A+H1*, A339S+G149A+I9M, A339S+G149A+K391A, A339S+G149A+M202L,A339S+G149A+N16Y, A339S+G149A+N195F, A339S+G149A+N54S,A339S+G149A+Q169E, A339S+G149A+Q169E, A339S+G149A+Q172K,A339S+G149A+Q299Y, A339S+G149A+Q345R, A339S+G149A+Q365S,A339S+G149A+R320K, A339S+G149A+R458K, A339S+G149A+S323T,A339S+G149A+V206L, A339S+G149A+Y295F, A339S+G182T+H1*, A339S+G182T+I9M,A339S+G182T+K391A, A339S+G182T+M202 L, A339S+G182T+N16Y,A339S+G182T+N195F, A339S+G182T+N54S, A339S+G182T+Q169E,A339S+G182T+Q169E, A339S+G182T+Q172K, A339S+G182T+Q299Y,A339S+G182T+Q345R, A339S+G182T+Q365S, A339S+G182T+R320K,A339S+G182T+R458K, A339S+G182T+S323T, A339S+G182T+V206L,A339S+G182T+Y295F, A339S+H1*+I9M, A339S+H1*+K391A, A339S+H 1*+M202L,A339S+H1*+N16Y, A339S+H1*+N195F, A339S+H1*+N54S, A339S+H1*+Q169E,A339S+H1*+Q169E, A339S+H1*+Q172K, A339S+H1*+Q299Y, A339S+H1*+Q345R,A339S+H1*+Q365S, A339S+H1*+R320K, A339S+H1*+R458K, A339S+H1*+S323T,A339S+H1*+V206L, A339S+H1*+Y295F, A339S+I9M+K391A, A339S+I9M+M202 L,A339S+I9M+N16Y, A339S+I9M+N195F, A339S+I9M+N54S, A339S+I9M+Q169E,A339S+I9M+Q169E, A339S+I9M+Q172K, A339S+I9M+Q299Y, A339S+I9M+Q345R,A339S+I9M+Q365S, A339S+I9M+R320K, A339S+I9M+R458K, A339S+I9M+S323T,A339S+I9M+V206L, A339S+I9M+Y295F, A339S+K391A+M202 L, A339S+K391A+N16Y,A339S+K391A+N195F, A339S+K391A+N54S, A339S+K391A+Q169E,A339S+K391A+Q169E, A339S+K391A+Q172K, A339S+K391A+Q299Y,A339S+K391A+Q345R, A339S+K391A+Q365S, A339S+K391A+R320K,A339S+K391A+R458K, A339S+K391A+S323T, A339S+K391A+V206L,A339S+K391A+Y295F, A339S+M202 L+N16Y, A339S+M202 L+N195F, A339S+M202L+N54S, A339S+M202L+Q169E, A339S+M202L+Q169E, A339S+M202L+Q172K,A339S+M202L+Q299Y, A339S+M202L+Q345R, A339S+M202L+Q365S,A339S+M202L+R320K, A339S+M202 L+R458K, A339S+M202L+S323T,A339S+M202L+V206L, A339S+M202L+Y295F, A339S+N16Y+N195F, A339S+N16Y+N54S,A339S+N16Y+Q169E, A339S+N16Y+Q169E, A339S+N16Y+Q172K, A339S+N16Y+Q299Y,A339S+N16Y+Q345R, A339S+N16Y+Q365S, A339S+N16Y+R320K, A339S+N16Y+R458K,A339S+N16Y+S323T, A339S+N16Y+V206L, A339S+N16Y+Y295F, A339S+N195F+N54S,A339S+N195F+Q169E, A339S+N195F+Q169E, A339S+N195F+Q172K,A339S+N195F+Q299Y, A339S+N195F+Q345R, A339S+N195F+Q365S,A339S+N195F+R320K, A339S+N195F+R458K, A339S+N195F+S323T,A339S+N195F+V206L, A339S+N195F+Y295F, A339S+N54S+Q169E,A339S+N54S+Q169E, A339S+N54S+Q172K, A339S+N54S+Q299Y, A339S+N54S+Q345R,A339S+N54S+Q365S, A339S+N54S+R320K, A339S+N54S+R458K, A339S+N54S+S323T,A339S+N54S+V206L, A339S+N54S+Y295F, A339S+Q169E+Q169E,A339S+Q169E+Q172K, A339S+Q169E+Q299Y, A339S+Q169E+Q345R,A339S+Q169E+Q365S, A339S+Q169E+R320K, A339S+Q169E+R458K,A339S+Q169E+S323T, A339S+Q169E+V206L, A339S+Q169E+Y295F,A339S+Q169E+Q172K, A339S+Q169E+Q299Y, A339S+Q169E+Q345R,A339S+Q169E+Q365S, A339S+Q169E+R320K, A339S+Q169E+R458 K,A339S+Q169E+S323T, A339S+Q169E+V206L, A339S+Q169E+Y295F,A339S+Q172K+Q299 Y, A339S+Q172K+Q345R, A339S+Q172K+Q365S,A339S+Q172K+R320K, A339S+Q172K+R45 8K, A339S+Q172K+S323T,A339S+Q172K+V206L, A339S+Q172K+Y295F, A339S+Q299Y+Q34 5R,A339S+Q299Y+Q365S, A339S+Q299Y+R320K, A339S+Q299Y+R458K, A339S+Q299Y+S323T, A339S+Q299Y+V206L, A339S+Q299Y+Y295F, A339S+Q345R+Q365S,A339S+Q345R+R3 20K, A339S+Q345R+R458K, A339S+Q345R+S323T,A339S+Q345R+V206L, A339S+Q345R+Y2 95F, A339S+Q365S+R320K,A339S+Q365S+R458K, 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N16Y+Q169E+Q169E,N16Y+Q169E+Q 172K, N16Y+Q169E+Q299Y, N16Y+Q169E+Q345R, N16Y+Q169E+Q365S,N16Y+Q169E+R320 K, N16Y+Q169E+R458K, N16Y+Q169E+S323T, N16Y+Q169E+V206L,N16Y+Q169E+Y295F, N1 6Y+Q169E+Q172K, N16Y+Q169E+Q299Y, N16Y+Q169E+Q345R,N16Y+Q169E+Q365S, N16Y+Q169E+R320K, N16Y+Q169E+R458K, N16Y+Q169E+S323T,N16Y+Q169E+V206L, N16Y+Q1 69E+Y295F, N16Y+Q172K+Q299Y, N16Y+Q172K+Q345R,N16Y+Q172K+Q365S, N16Y+Q172K+R320K, N16Y+Q172K+R458K, N16Y+Q172K+S323T,N16Y+Q172K+V206L, N16Y+Q172K+Y2 95F, N16Y+Q299Y+Q345R, N16Y+Q299Y+Q365S,N16Y+Q299Y+R320K, N16Y+Q299Y+R458K, N16Y+Q299Y+S323T, N16Y+Q299Y+V206L,N16Y+Q299Y+Y295F, N16Y+Q345R+Q365S, N16 Y+Q345R+R320K, N16Y+Q345R+R458K,N16Y+Q345R+S323T, N16Y+Q345R+V206L, N16Y+Q 345R+Y295F, N16Y+Q365S+R320K,N16Y+Q365S+R458K, N16Y+Q365S+S323T, N16Y+Q365 S+V206L, N16Y+Q365S+Y295F,N16Y+R320K+R458K, N16Y+R320K+S323T, N16Y+R320K+V2 06L, N16Y+R320K+Y295F,N16Y+R458K+S323T, N16Y+R458K+V206L, N16Y+R458K+Y295F, N 16Y+S323T+V206L,N16Y+S323T+Y295F, N16Y+V206L+Y295F, N195F+N54S+Q169E, N195F+N54S+Q169E,N195F+N54S+Q172K, N195F+N54S+Q299Y, N195F+N54S+Q345R, N195F+N54 S+Q365S,N195F+N54S+R320K, N195F+N54S+R458K, N195F+N54S+S323T, N195F+N54S+V2 06L,N195F+N54S+Y295F, N195F+Q169E+Q169E, N195F+Q169E+Q172K, N195F+Q169E+Q299Y, N195F+Q169E+Q345R, N195F+Q169E+Q365S, N195F+Q169E+R320K,N195F+Q169E+R4 58K, N195F+Q169E+S323T, N195F+Q169E+V206L,N195F+Q169E+Y295F, N195F+Q169E+Q1 72K, N195F+Q169E+Q299Y,N195F+Q169E+Q345R, N195F+Q169E+Q365S, N195F+Q169E+R 320K,N195F+Q169E+R458K, N195F+Q169E+S323T, N195F+Q169E+V206L, N195F+Q169E+Y295F, N195F+Q172K+Q299Y, N195F+Q172K+Q345R, N195F+Q172K+Q365S,N195F+Q172K+R320K, N195F+Q172K+R458K, N195F+Q172K+S323T,N195F+Q172K+V206L, N195F+Q172K+Y295F, N195F+Q299Y+Q345R,N195F+Q299Y+Q365S, N195F+Q299Y+R320K, N195F+Q299Y+R458K,N195F+Q299Y+S323T, N195F+Q299Y+V206L, N195F+Q299Y+Y295F,N195F+Q345R+Q365S, N195F+Q345R+R320K, N195F+Q345R+R458K,N195F+Q345R+S323T, N195F+Q345 R+V206L, N195F+Q345R+Y295F,N195F+Q365S+R320K, N195F+Q365S+R458K, N195F+Q365 S+S323T,N195F+Q365S+V206LN195F+Q365S+Y295F, N195F+R320K+R458K,N195F+R320K+S323T, N195F+R320K+V206L, N195F+R320K+Y295F,N195F+R458K+S323T, N195F+R458K+V206L, N195F+R458K+Y295F,N195F+S323T+V206L, N195F+S323T+Y295F, N195F+V206L+Y 295F,N54S+Q169E+Q169E, N54S+Q169E+Q172K, N54S+Q169E+Q299Y, N54S+Q169E+Q345 R,N54S+Q169E+Q365S, N54S+Q169E+R320K, N54S+Q169E+R458K, N54S+Q169E+S323T,N 54S+Q169E+V206L, N54S+Q169E+Y295F, N54S+Q169E+Q172K, N54S+Q169E+Q299Y,N54S+Q169E+Q345R, N54S+Q169E+Q365S, N54S+Q169E+R320K, N54S+Q169E+R458K,N54S+Q 169E+S323T, N54S+Q169E+V206L, N54S+Q169E+Y295F, N54S+Q172K+Q299Y,N54S+Q172K+Q345R, N54S+Q172K+Q365S, N54S+Q172K+R320K, N54S+Q172K+R458K,N54S+Q172K+S3 23T, N54S+Q172K+V206L, N54S+Q172K+Y295F, N54S+Q299Y+Q345R,N54S+Q299Y+Q365S, N54S+Q299Y+R320K, N54S+Q299Y+R458K, N54S+Q299Y+S323T,N54S+Q299Y+V206L, N54 S+Q299Y+Y295F, N54S+Q345R+Q365S, N54S+Q345R+R320K,N54S+Q345R+R458K, N54S+Q345R+S323T, N54S+Q345R+V206L, N54S+Q345R+Y295F,N54S+Q365S+R320K, N54S+Q36 5S+R458K, N54S+Q365S+S323T, N54S+Q365S+V206L,N54S+Q365S+Y295F, N54S+R320K+R458K, N54S+R320K+S323T, N54S+R320K+V206L,N54S+R320K+Y295F, N54S+R458K+S323 T, N54S+R458K+V206L, N54S+R458K+Y295F,N54S+S323T+V206L, N54S+S323T+Y295F, N54 S+V206L+Y295F,Q169E+Q169E+Q172K, Q169E+Q169E+Q299Y, Q169E+Q169E+Q345R, Q169E+Q169E+Q365S, Q169E+Q169E+R320K, Q169E+Q169E+R458K, Q169E+Q169E+S323T,Q1 69E+Q169E+V206L, Q169E+Q169E+Y295F, Q169E+Q172K+Q299Y,Q169E+Q172K+Q345R, Q 169E+Q172K+Q365S, Q169E+Q172K+R320K,Q169E+Q172K+R458K, Q169E+Q172K+S323T, Q169E+Q172K+V206L,Q169E+Q172K+Y295F, Q169E+Q299Y+Q345R, Q169E+Q299Y+Q365 S,Q169E+Q299Y+R320K, Q169E+Q299Y+R458K, Q169E+Q299Y+S323T, Q169E+Q299Y+V206L, Q169E+Q299Y+Y295F, Q169E+Q345R+Q365S, Q169E+Q345R+R320K,Q169E+Q345R+R4 58K, Q169E+Q345R+S323T, Q169E+Q345R+V206L,Q169E+Q345R+Y295F, Q169E+Q365S+R 320K, Q169E+Q365S+R458K,Q169E+Q365S+S323T, Q169E+Q365S+V206L, Q169E+Q365S+Y295F,Q169E+R320K+R458K, Q169E+R320K+S323T, Q169E+R320K+V206L,Q169E+R320K+Y295F, Q169E+R458K+S323T, Q169E+R458K+V206L,Q169E+R458K+Y295F, Q169E+S323T+V206L, Q169E+S323T+Y295F,Q169E+V206L+Y295F, Q169E+Q172K+Q299Y, Q169E+Q172K+Q345R,Q169E+Q172K+Q365S, Q169E+Q172K+R320K, Q169E+Q172K+R458K,Q169E+Q172K+S323T, Q169E+Q172K+V206L, Q169E+Q172K+Y295F,Q169E+Q299Y+Q345R, Q169E+Q299 Y+Q365S, Q169E+Q299Y+R320K,Q169E+Q299Y+R458K, Q169E+Q299Y+S323T, Q169E+Q29 9Y+V206L,Q169E+Q299Y+Y295F, Q169E+Q345R+Q365S, Q169E+Q345R+R320K, Q169E+Q345R+R458K, Q169E+Q345R+S323T, Q169E+Q345R+V206L, Q169E+Q345R+Y295F,Q169E+Q 365S+R320K, Q169E+Q365S+R458K, Q169E+Q365S+S323T,Q169E+Q365S+V206L, Q169E+Q365S+Y295F, Q169E+R320K+R458K,Q169E+R320K+S323T, Q169E+R320K+V206L, Q169E+R320K+Y295F,Q169E+R458K+S323T, Q169E+R458K+V206L, Q169E+R458K+Y295F,Q169E+S323T+V206L, Q169E+S323T+Y295F, Q169E+V206L+Y295F,Q172K+Q299Y+Q345R, Q172K+Q299Y+Q365S, Q172K+Q299Y+R320K,Q172K+Q299Y+R458K, Q172K+Q299Y+S323T, Q172K+Q299Y+V206L,Q172K+Q299Y+Y295F, Q172K+Q345R+Q365S, Q172K+Q345R+R320K, Q172K+Q345R+R458K, Q172K+Q345R+S323T, Q172K+Q345R+V206L, Q172K+Q345R+Y295F,Q17 2K+Q365S+R320K, Q172K+Q365S+R458K, Q172K+Q365S+S323T,Q172K+Q365S+V206L, Q1 72K+Q365S+Y295F, Q172K+R320K+R458K,Q172K+R320K+S323T, Q172K+R320K+V206L, Q1 72K+R320K+Y295F,Q172K+R458K+S323T, Q172K+R458K+V206L, Q172K+R458K+Y295F, Q172K+S323T+V206L, Q172K+S323T+Y295F, Q172K+V206L+Y295F,Q299Y+Q345R+Q365S, Q2 99Y+Q345R+R320K, Q299Y+Q345R+R458K,Q299Y+Q345R+S323T, Q299Y+Q345R+V206L, Q 299Y+Q345R+Y295F,Q299Y+Q365S+R320K, Q299Y+Q365S+R458K, Q299Y+Q365S+S323T,Q299Y+Q365S+V206L, Q299Y+Q365S+Y295F, Q299Y+R320K+R458K,Q299Y+R320K+S323T, Q299Y+R320K+V206L, Q299Y+R320K+Y295F,Q299Y+R458K+S323T, Q299Y+R458K+V206L, Q299Y+R458K+Y295F,Q299Y+S323T+V206L, Q299Y+S323T+Y295F, Q299Y+V206L+Y295F,Q345R+Q365S+R320K, Q345R+Q365S+R458K, Q345R+Q365S+S323T,Q345R+Q365S+V206 L, Q345R+Q365S+Y295F, Q345R+R320K+R458K,Q345R+R320K+S323T, Q345R+R320K+V20 6L, Q345R+R320K+Y295F,Q345R+R458K+S323T, Q345R+R458K+V206L, Q345R+R458K+Y29 5F,Q345R+S323T+V206L, Q345R+S323T+Y295F, Q345R+V206L+Y295F, Q365S+R320K+R458K, Q365S+R320K+S323T, Q365S+R320K+V206L, Q365S+R320K+Y295F,Q365S+R458K+S32 3T, Q365S+R458K+V206L, Q365S+R458K+Y295F,Q365S+S323T+V206L, Q365S+S323T+Y295F, Q365S+V206L+Y295F,R320K+R458K+S323T, R320K+R458K+V206L, R320K+R458K+Y295F,R320K+S323T+V206L, R320K+S323T+Y295F, R320K+V206L+Y295F,R458K+S323T+V206L, R458K+S323T+Y295F, R458K+V206L+Y295F,S323T+V206L+Y295F, using SEQ ID NO: 1 for numbering and wherein saidvariant has at least 60%, at least 65%, at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, or at least 99%, but less than 100% sequenceidentity to the polypeptide of SEQ ID Nos; 1-17. In one aspect, thevariant comprises one or more of the following modification at aposition corresponding to positions:

A174*+A339S+G109A+G149A, A174*+A339S+G109A+G182T, A174*+A339S+G109A+H1*,A17 4*+A339S+G109A+I9M, A174*+A339S+G109A+K391A,A174*+A339S+G109A+M202L, A174*+A339S+G109A+N16Y,A174*+A339S+G109A+N195F, A174*+A339S+G109A+N54S, A174*+A3395+G109A+Q169E, A174*+A339S+G109A+Q169E, A174*+A339S+G109A+Q172K,A174*+A339 S+G109A+Q299Y, A174*+A339S+G109A+Q345R,A174*+A339S+G109A+Q365S, A174*+A339 S+G109A+R320K,A174*+A339S+G109A+R458K, A174*+A339S+G109A+S323T,A174*+A339S+G109A+V206L, A174*+A339S+G109A+Y295F,A174*+A339S+G149A+G182T, A174*+A339S+G149A+H1*, A174*+A339S+G149A+I9M,A174*+A339S+G149A+K391A, A174*+A339S+G149A+M202L,A174*+A339S+G149A+N16Y, A174*+A339S+G149A+N195F, A174*+A339S+G149A+N54S, A174*+A339S+G149A+Q169E, A174*+A339S+G149A+Q169E,A174*+A339S+G149A+Q17 2K, A174*+A339S+G149A+Q299Y,A174*+A339S+G149A+Q345R, A174*+A339S+G149A+Q36 5S,A174*+A339S+G149A+R320K, A174*+A339S+G149A+R458K, A174*+A339S+G149A+S323T, A174*+A339S+G149A+V206L, A174*+A339S+G149A+Y295F,A174*+A339S+G182T+H1*, A1 74*+A339S+G182T+I9M, A174*+A339S+G182T+K391A,A174*+A339S+G182T+M202 L, A174*+A339S+G182T+N16Y,A174*+A339S+G182T+N195F, A174*+A339S+G182T+N54S, A174*+A3395+G182T+Q169E, A174*+A339S+G182T+Q169E, A174*+A339S+G182T+Q172K,A174*+A339 S+G182T+Q299Y, A174*+A339S+G182T+Q345R,A174*+A339S+G182T+Q365S, A174*+A339S+G182T+R320K,A174*+A339S+G182T+R458K, A174*+A339S+G182T+S323T,A174*+A339S+G182T+V206L, A174*+A339S+G182T+Y295F, A174*+A339S+H1*+I9M,A174*+A339S+H1*+K3 91A, A174*+A339S+H1*+M202L, A174*+A339S+H1*+N16Y,A174*+A339S+H1*+N195F, A174*+A339S+H1*+N54S, A174*+A339S+H1*+Q169E,A174*+A339S+H1*+Q169E, A174*+A339S+H1*+Q172K, A174*+A339S+H1*+Q299Y,A174*+A339S+H1*+Q345R, A174*+A339S+H1*+Q365S, A 174*+A339S+H1*+R320K,A174*+A339S+H1*+R458K, A174*+A339S+H1*+S323T, A174*+A339 S+H1*+V206L,A174*+A339S+H1*+Y295F, A174*+A339S+I9M+K391A, A174*+A339S+I9M+M2 02 L,A174*+A339S+I9M+N16Y, A174*+A339S+I9M+N195F, A174*+A339S+I9M+N54S,A174*+A 339S+I9M+Q169E, A174*+A339S+I9M+Q169E, A174*+A339S+I9M+Q172K,A174*+A339S+I9M+Q299Y, A174*+A339S+I9M+Q345R, A174*+A339S+I9M+Q365S,A174*+A339S+I9M+R320K, A 174*+A339S+I9M+R458K, A174*+A339S+I9M+S323T,A174*+A339S+I9M+V206L, A174*+A339 S+I9M+Y295F, A174*+A339S+K391A+M202 L,A174*+A339S+K391A+N16Y, A174*+A339S+K39 1A+N195F,A174*+A339S+K391A+N54S, A174*+A339S+K391A+Q169E,A174*+A339S+K391A+Q169E, A174*+A339S+K391A+Q172K,A174*+A339S+K391A+Q299Y, A174*+A339S+K391A+Q345R,A174*+A339S+K391A+Q365S, A174*+A339S+K391A+R320K, A174*+A339S+K391A+R458K, A174*+A339S+K391A+S323T, A174*+A339S+K391A+V206L,A174*+A339S+K391A+Y29 5F, A174*+A339S+M202 L+N16Y,A174*+A339S+M202L+N195F, A174*+A339S+M202L+N54S, A174*+A339S+M202L+Q169E, A174*+A339S+M202 L+Q169E, A174*+A339S+M202L+Q172K, A174*+A339S+M202L+Q299Y, A174*+A339S+M202L+Q345R,A174*+A339S+M202L+Q365S, A1 74*+A339S+M202 L+R320K, A174*+A339S+M202L+R458K, A174*+A339S+M202L+S323T, A17 4*+A339S+M202L+V206L,A174*+A339S+M202 L+Y295F, A174*+A339S+N16Y+N195F, A174*+A339S+N16Y+N54S,A174*+A339S+N16Y+Q169E, A174*+A339S+N16Y+Q169E, A174*+A339S+N16Y+Q172K,A174*+A339S+N16Y+Q299Y, A174*+A339S+N16Y+Q345R, A174*+A339S+N1 6Y+Q365S,A174*+A339S+N16Y+R320K, A174*+A339S+N16Y+R458K, A174*+A339S+N16Y+S 323T,A174*+A339S+N16Y+V206L, A174*+A339S+N16Y+Y295F, A174*+A339S+N195F+N54S,A174*+A339S+N195F+Q169E, A174*+A339S+N195F+Q169E,A174*+A339S+N195F+Q172K, A 174*+A339S+N195F+Q299Y,A174*+A339S+N195F+Q345R, A174*+A339S+N195F+Q365S, A174*+A339S+N195F+R320K, A174*+A339S+N195F+R458K, A174*+A339S+N195F+S323T,A174*+A339S+N195F+V206L, A174*+A339S+N195F+Y295F,A174*+A339S+N54S+Q169E, A174*+A 339S+N54S+Q169E, A174*+A339S+N54S+Q172K,A174*+A339S+N54S+Q299Y, A174*+A339S+N54S+Q345R, A174*+A339S+N54S+Q365S,A174*+A339S+N54S+R320K, A174*+A339S+N5 4S+R458K, A174*+A339S+N54S+S323T,A174*+A339S+N54S+V206L, A174*+A339S+N54S+Y 295F,A174*+A339S+Q169E+Q169E, A174*+A339S+Q169E+Q172K, A174*+A339S+Q169E+Q299Y, A174*+A339S+Q169E+Q345R, A174*+A339S+Q169E+Q365S,A174*+A339S+Q169E+R32 0K, A174*+A339S+Q169E+R458K,A174*+A339S+Q169E+S323T, A174*+A339S+Q169E+V206 L,A174*+A339S+Q169E+Y295F, A174*+A339S+Q169E+Q172K,A174*+A339S+Q169E+Q299Y, A174*+A339S+Q169E+Q345R,A174*+A339S+Q169E+Q365S, A174*+A339S+Q169E+R320K,A174*+A339S+Q169E+R458K, A174*+A339S+Q169E+S323T,A174*+A339S+Q169E+V206L, A 174*+A339S+Q169E+Y295F,A174*+A339S+Q172K+Q299Y, A174*+A339S+Q172K+Q345R, A174*+A339S+Q172K+Q365S, A174*+A339S+Q172K+R320K, A174*+A339S+Q172K+R458K,A17 4*+A339S+Q172K+S323T, A174*+A339S+Q172K+V206L,A174*+A339S+Q172K+Y295F, A174*+A339S+Q299Y+Q345R,A174*+A339S+Q299Y+Q365S, A174*+A339S+Q299Y+R320K,A174*+A339S+Q299Y+R458K, A174*+A339S+Q299Y+S323T,A174*+A339S+Q299Y+V206L, A174*+A339S+Q299Y+Y295F,A174*+A339S+Q345R+Q365S, A174*+A339S+Q345R+R320K, A174*+A339S+Q345R+R458K, A174*+A339S+Q345R+S323T, A174*+A339S+Q345R+V206L,A174*+A3 39S+Q345R+Y295F, A174*+A339S+Q365S+R320K,A174*+A339S+Q365S+R458K, A174*+A33 9S+Q365S+S323T,A174*+A339S+Q365S+V206L, A174*+A339S+Q365S+Y295F, A174*+A339S+R320K+R458K, A174*+A339S+R320K+S323T, A174*+A339S+R320K+V206L,A174*+A339S+R320K+Y295F, A174*+A339S+R458K+S323T,A174*+A339S+R458K+V206L, A174*+A339S+R458K+Y295F,A174*+A339S+S323T+V206L, A174*+A339S+S323T+Y295F, A174*+A339S+V206L+Y295F, A174*+G109A+G149A+G182T, A174*+G109A+G149A+H1*,A174*+G109A+G149A+I9M, A174*+G109A+G149A+K391A, A174*+G109A+G149A+M202L,A174*+G109A+G149A+N 16Y, A174*+G109A+G149A+N195F,A174*+G109A+G149A+N54S, A174*+G109A+G149A+Q16 9E,A174*+G109A+G149A+Q169E, A174*+G109A+G149A+Q172K, A174*+G109A+G149A+Q299Y, A174*+G109A+G149A+Q345R, A174*+G109A+G149A+Q365S,A174*+G109A+G149A+R32 0K, A174*+G109A+G149A+R458K,A174*+G109A+G149A+S323T, A174*+G109A+G149A+V206 L,A174*+G109A+G149A+Y295F, A174*+G109A+G182T+H1*, A174*+G109A+G182T+I9M,A174*+G109A+G182T+K391A, A174*+G109A+G182T+M202L,A174*+G109A+G182T+N16Y, A174*+G109A+G182T+N195F, A174*+G109A+G182T+N54S,A174*+G109A+G182T+Q169E, A174*+G 109A+G182T+Q169E,A174*+G109A+G182T+Q172K, A174*+G109A+G182T+Q299Y, A174*+G109A+G182T+Q345R, A174*+G109A+G182T+Q365S, A174*+G109A+G182T+R320K,A174*+G 109A+G182T+R458K, 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299Y+R458K+Y295F,Q169E+Q299Y+S323T+V206L, Q169E+Q299Y+S323T+Y295F, Q169E+Q299Y+V206L+Y295F, Q169E+Q345R+Q365S+R320K, Q169E+Q345R+Q365S+R458K,Q169E+Q345R+Q365S+S323T, Q169E+Q345R+Q365S+V206L,Q169E+Q345R+Q365S+Y295F, Q169E+Q345R+R320K+R458K,Q169E+Q345R+R320K+S323T, Q169E+Q345R+R320K+V206L, Q169E+Q345R+R320K+Y295F, Q169E+Q345R+R458K+S323T, Q169E+Q345R+R458K+V206L,Q16 9E+Q345R+R458K+Y295F, Q169E+Q345R+S323T+V206L,Q169E+Q345R+S323T+Y295F, Q1 69E+Q345R+V206L+Y295F,Q169E+Q365S+R320K+R458K, Q169E+Q365S+R320K+S323T, Q169E+Q365S+R320K+V206L, Q169E+Q365S+R320K+Y295F,Q169E+Q365S+R458K+S323T, Q169E+Q365S+R458K+V206L,Q169E+Q365S+R458K+Y295F, Q169E+Q365S+S323T+V206L,Q169E+Q365S+S323T+Y295F, Q169E+Q365S+V206L+Y295F,Q169E+R320K+R458K+S323T, Q169E+R320K+R458K+V206L,Q169E+R320K+R458K+Y295F, Q169E+R320K+S323T+V206L,Q169E+R320K+S323T+Y295F, Q169E+R320K+V206L+Y295F,Q169E+R458K+S323T+V206L, Q169E+R458K+S323T+Y295F,Q169E+R458K+V206L+Y295F, Q169E+S323T+V206L+Y295F,Q169E+Q172K+Q299Y+Q345R, Q169E+Q172K+Q299Y+Q365S, Q169E+Q172K+Q299Y+R320K, Q169E+Q172K+Q299Y+R458K, Q169E+Q172K+Q299Y+S323T,Q169E+Q172K+Q299Y+V2 06L, Q169E+Q172K+Q299Y+Y295F,Q169E+Q172K+Q345R+Q365S, Q169E+Q172K+Q345R+R320K,Q169E+Q172K+Q345R+R458K, Q169E+Q172K+Q345R+S323T, Q169E+Q172K+Q345R+V206L, Q169E+Q172K+Q345R+Y295F, Q169E+Q172K+Q365S+R320K,Q169E+Q172K+Q3 65S+R458K, Q169E+Q172K+Q365S+S323T,Q169E+Q172K+Q365S+V206L, Q169E+Q172K+Q 365S+Y295F,Q169E+Q172K+R320K+R458K, Q169E+Q172K+R320K+S323T,Q169E+Q172K+R320K+V206L, Q169E+Q172K+R320K+Y295F,Q169E+Q172K+R458K+S323T, Q169E+Q172K+R458K+V206L,Q169E+Q172K+R458K+Y295F, Q169E+Q172K+S323T+V206L, Q169E+Q172K+S323T+Y295F, Q169E+Q172K+V206L+Y295F, Q169E+Q299Y+Q345R+Q365S,Q169E+Q29 9Y+Q345R+R320K, Q169E+Q299Y+Q345R+R458K,Q169E+Q299Y+Q345R+S323T, Q169E+Q 299Y+Q345R+V206L,Q169E+Q299Y+Q345R+Y295F, Q169E+Q299Y+Q365S+R320K,Q169E+Q299Y+Q365S+R458K, Q169E+Q299Y+Q365S+S323T,Q169E+Q299Y+Q365S+V206L, Q169E+Q299Y+Q365S+Y295F,Q169E+Q299Y+R320K+R458K, Q169E+Q299Y+R320K+S323T, Q169E+Q299Y+R320K+V206L, Q169E+Q299Y+R320K+Y295F, Q169E+Q299Y+R458K+S323T,Q16 9E+Q299Y+R458K+V206L, Q169E+Q299Y+R458K+Y295F,Q169E+Q299Y+S323T+V206L, Q1 69E+Q299Y+S323T+Y295F,Q169E+Q299Y+V206L+Y295F, Q169E+Q345R+Q365S+R320K, Q169E+Q345R+Q365S+R458K, Q169E+Q345R+Q365S+S323T,Q169E+Q345R+Q365S+V206L, Q169E+Q345R+Q365S+Y295F,Q169E+Q345R+R320K+R458K, Q169E+Q345R+R320K+S323 T,Q169E+Q345R+R320K+V206L, Q169E+Q345R+R320K+Y295F, Q169E+Q345R+R458K+S323T, Q169E+Q345R+R458K+V206L, Q169E+Q345R+R458K+Y295F,Q169E+Q345R+S323T+V2 06L, Q169E+Q345R+S323T+Y295F,Q169E+Q345R+V206L+Y295F, Q169E+Q365S+R320K+R 458K,Q169E+Q365S+R320K+S323T, Q169E+Q365S+R320K+V206L,Q169E+Q365S+R320K+Y295F, Q169E+Q365S+R458K+S323T,Q169E+Q365S+R458K+V206L, Q169E+Q365S+R458K+Y295F,Q169E+Q365S+S323T+V206L, Q169E+Q365S+S323T+Y295F,Q169E+Q365S+V206L+Y295F, Q169E+R320K+R458K+S323T,Q169E+R320K+R458K+V206L, Q169E+R320K+R458K+Y295F,Q169E+R320K+S323T+V206L, Q169E+R320K+S323T+Y295F,Q169E+R320K+V206L+Y295F, Q169E+R458K+S323T+V206L,Q169E+R458K+S323T+Y295F, Q169E+R458K+V206L+Y295F,Q169E+S323T+V206L+Y295F, Q172K+Q299Y+Q345R+Q365S,Q172K+Q299Y+Q345R+R320K, Q172K+Q299Y+Q345R+R458K, Q172K+Q299Y+Q345R+S323T, Q172K+Q299Y+Q345R+V206L, Q172K+Q299Y+Q345R+Y295F,Q172K+Q299Y+Q365S+R32 0K, Q172K+Q299Y+Q365S+R458K,Q172K+Q299Y+Q365S+S323T, Q172K+Q299Y+Q365S+V 206L,Q172K+Q299Y+Q365S+Y295F, Q172K+Q299Y+R320K+R458K,Q172K+Q299Y+R320K+S323T, Q172K+Q299Y+R320K+V206L,Q172K+Q299Y+R320K+Y295F, Q172K+Q299Y+R458K+S323T,Q172K+Q299Y+R458K+V206L, Q172K+Q299Y+R458K+Y295F, Q172K+Q299Y+S323T+V206L, Q172K+Q299Y+S323T+Y295F, Q172K+Q299Y+V206L+Y295F,Q172K+Q345R+Q365 S+R320K, Q172K+Q345R+Q365S+R458K,Q172K+Q345R+Q365S+S323T, Q172K+Q345R+Q3 65S+V206L,Q172K+Q345R+Q365S+Y295F, Q172K+Q345R+R320K+R458K, Q172K+Q345R+R320K+S323T, Q172K+Q345R+R320K+V206L, Q172K+Q345R+R320K+Y295F,Q172K+Q345R+R458K+S323T, Q172K+Q345R+R458K+V206L,Q172K+Q345R+R458K+Y295F, Q172K+Q345R+S323T+V206L,Q172K+Q345R+S323T+Y295F, Q172K+Q345R+V206L+Y295F,Q172K+Q365S+R320K+R458K, Q172K+Q365S+R320K+S323T,Q172K+Q365S+R320K+V206L, Q172K+Q365 S+R320K+Y295F,Q172K+Q365S+R458K+S323T, Q172K+Q365S+R458K+V206L, Q172K+Q365S+R458K+Y295F, Q172K+Q365S+S323T+V206L, Q172K+Q365S+S323T+Y295F,Q172K+Q36 5S+V206L+Y295F, Q172K+R320K+R458K+S323T,Q172K+R320K+R458K+V206L, Q172K+R320K+R458K+Y295F,Q172K+R320K+S323T+V206L, Q172K+R320K+S323T+Y295F, Q172K+R320K+V206L+Y295F, Q172K+R458K+S323T+V206L, Q172K+R458K+S323T+Y295F,Q172K+R45 8K+V206L+Y295F, Q172K+S323T+V206L+Y295F,Q299Y+Q345R+Q365S+R320K, Q299Y+Q34 5R+Q365S+R458K,Q299Y+Q345R+Q365S+S323T, Q299Y+Q345R+Q365S+V206L, Q299Y+Q345R+Q365S+Y295F, Q299Y+Q345R+R320K+R458K, Q299Y+Q345R+R320K+S323T,Q299Y+Q345R+R320K+V206L, Q299Y+Q345R+R320K+Y295F,Q299Y+Q345R+R458K+S323T, Q299Y+Q345R+R458K+V206L,Q299Y+Q345R+R458K+Y295F, Q299Y+Q345R+S323T+V206L, Q299Y+Q345R+S323T+Y295F, Q299Y+Q345R+V206L+Y295F, Q299Y+Q365S+R320K+R458K,Q29 9Y+Q365S+R320K+S323T, Q299Y+Q365S+R320K+V206L,Q299Y+Q365S+R320K+Y295F, Q2 99Y+Q365S+R458K+S323T,Q299Y+Q365S+R458K+V206 L, Q299Y+Q365S+R458K+Y295F, Q299Y+Q365S+S323T+V206L, Q299Y+Q365S+S323T+Y295F,Q299Y+Q365S+V206L+Y295F, Q 299Y+R320K+R458K+S323T,Q299Y+R320K+R458K+V206L, Q299Y+R320K+R458K+Y295F,Q299Y+R320K+S323T+V206L, Q299Y+R320K+S323T+Y295F, Q299Y+R320K+V206L+Y295F, Q299Y+R458K+S323T+V206L, Q299Y+R458K+S323T+Y295F,Q299Y+R458K+V206L+Y295F, Q299Y+S323T+V206L+Y295F,Q345R+Q365S+R320K+R458K, Q345R+Q365S+R320K+S323T,Q345R+Q365S+R320K+V206L, Q345R+Q365S+R320K+Y295F, Q345R+Q365S+R458K+S323T, Q345R+Q365S+R458K+V206L, Q345R+Q365S+R458K+Y295F,Q345R+Q365S+S323T+V20 6L, Q345R+Q365S+S323T+Y295F,Q345R+Q365S+V206L+Y295F, Q345R+R320K+R458K+S3 23T,Q345R+R320K+R458K+V206L, Q345R+R320K+R458K+Y295F, Q345R+R320K+S323T+V206L, Q345R+R320K+S323T+Y295F, Q345R+R320K+V206L+Y295F,Q345R+R458K+S323T+V 206L, Q345R+R458K+S323T+Y295F,Q345R+R458K+V206L+Y295F, Q345R+S323T+V206L+Y 295F,Q365S+R320K+R458K+S323T, Q365S+R320K+R458K+V206L,Q365S+R320K+R458K+Y295F, Q365S+R320K+S323T+V206L,Q365S+R320K+S323T+Y295F, Q365S+R320K+V206L+Y295F,Q365S+R458K+S323T+V206L, Q365S+R458K+S323T+Y295F,Q365S+R458K+V206L+Y295F, Q365S+S323T+V206L+Y295F,R320K+R458K+S323T+V206L, R320K+R458K+S323T+Y295F, R320K+R458K+V206L+Y295F, R320K+S323T+V206 L+Y295F, R458K+S323T+V206 L+Y 295F, using SEQID NO: 1 for numbering and wherein said variant has at least 60%, atleast 65%, at least 70%, at least 75%, at least 80%, at least 85%, atleast 90%, at least 95%, at least 96%, at least 97%, at least 98%, or atleast 99%, but less than 100% sequence identity to the polypeptide ofSEQ ID Nos; 1-17.

In one aspect, the variant comprises one or more of the followingmodification at a position corresponding to positions:H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 95%, at least 96%, at least 97%, at least98%, or at least 99%, but less than 100% sequence identity to thepolypeptide of SEQ ID Nos; 1-17.

The inventors of the present invention have identified that thesespecific modification at these positions of the amino acid sequence asset forth in SEQ ID NO: 1 or 2, are particularly relevant for improvingthe performance of a variant alpha-amylase having at least 60% sequenceidentity to the parent polypeptide.

According to the present invention, a value of 1.0 corresponds to theperformance observed for the parent polypeptide. A value above 1.0indicates an improvement of performance of the variant tested comparedto the parent polypeptide. Accordingly, any value of >1.0 is indicativefor improvement of property, such as performance, of the variantcompared to the parent polypeptide.

According to the present invention, a variant showing improvement ofproperty under at least one condition tested, is considered a varianthaving improved property as compared to the parent polypeptide.

As stated elsewhere herein, the parent polypeptide may be anypolypeptide having alpha-amylase activity and at least 60% sequenceidentity to any one of the amino acid sequences as set forth in SEQ IDNO: 2.

As can be seen from the data obtained in Examples 3, 4 and 5, all testedvariants have an Improvement Factor (IF) of at least 1.1, i.e. above theparent polypeptide having SEQ ID NO: 2, when tested in either

-   -   (i) a Model A detergent and an enzyme concentration of 0.1 mg/L,    -   (ii) a ADW detergent Model at 45° C. and an enzyme concentration        of 0.35 mg/L,    -   (iii) a ADW detergent Model at 40° C. and an enzyme        concentration of 1.5 mg/L, or    -   (iv) a ADW detergent at 45° C. and an enzyme concentration of        0.9 mg/L.

In one embodiment, the variant has an improved wash performance, theenhanced wash performance corresponding to an Improvement Factor (IF) ofat least 1.1, preferably at least 1.2, at least 1.3, at least 1.4, atleast 1.5, at least 1.6, at least 1.7, at least 1.8, at least 1.9, atleast 2.0, at least 2.2, at least 2.4, at least 2.6, at least 2.8, atleast 3.0, at least 3.2, at least 3.4, at least 3.6, at least 3.8, or atleast 4.0 when compared to said parent alpha-amylase havingalpha-amylase activity wherein said IF is determined by using Model Adetergent composition and/or ADW detergent composition and wherein saidparent alpha-amylase has amino acid sequence as shown in SEQ ID NO: 1 orSEQ ID NO: 2.

In one aspect, the variant is selected from a group consisting of:

A60T Y135H Q169E W48Y A174* M246L R90Q Y203L+V206L Q169F+S170AS132D+G133D N195F+Q365C N195F+Q365S N195F+Q365M R127L+Q365S R127N+Q365SR127L+Q365C N195F+Q365K I9M+N195F R171H+Q365C Y267F+Q365S F328L+Q365SR171L+Q365S N195F+M286L T134P+N195F S170*+R171*+A174* V264I+A265G+Y267FQ169*+S170*+A174* M246L+L275V+Q365S V264I+V291I+Q365S V264I+V291T+Q365KV264I+F328L+Q365C I9M+N195F+Q365C Q11H+Y267F+Q365C I9M+N195F+Q365SV264I+Y267F+Q365K R127L+M246V+Q365S A60S+R171Y+Q365S R127N+M246T+L275VV104A+N195F+Q365S V264I+F328L+Q365S R171H+N302H+Q365S V264I+Y267F+Q365SA174*+N175*+Y178F V264I+A265G+Q365C R171L+N302H+Q365C R171L+M246V+Q365CR171Y+M246L+Q365S V264T+Y267F+Q365S I9M+Y160H+N195F+Q365SI9M+R127N+N195F+Q365S R127H+L235I+Q365S+G4350 I9M+R171H+N195F+Q365SI9M+A186H+N195F+Q365S I9M+R171L+N195F+Q365S Q169*+S170*+R171*+A174*M246L+L275V+Q365S+D406H I9M+G184K+N195F+Q365S R127N+M246L+L275V+Q365SR171H+M246L+L275V+Q365S R127H+M246I+Q365C+V481A Y267F+V291A+F328L+Q365CI9M+A186E+N195F+Q365S E86L+R171L+N306R+Q365S 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I9M+N195F+Q365CI9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458KI9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365SI9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S I9M+R171L+N195F+Q365SI9M+Y160H+N195F+Q365S M202L M246L+L275V+Q365S+D406H M246LM246L+L275V+Q365S M246L+L275V+Q365S+D406HN16E+N195F+G337D+Q365S+P375K+D377N N16E+N195F+G337E+Q365S+P375T+D377HN16E+N195F+G337E+Q365S+P375T+D377HN16Y+Y160H+N195F+L275I+S304G+G337E+Q365SN16Y+Y160H+N195F+L275I+S304G+G337E+Q365S N195F+G337D+Q365S+P375K+D377NN195F+G337D+Q365S+P375K+D377Y N195F+G337E+Q365S+P375K+D377NN195F+G337D+Q365S+P375K+D377Y N195F+G337E+Q365S+P375K+D377N N195F+Q365SN54Q+F113T+R171H+N302A+Q365CN54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KQ11H+V264I+A265G+Q365C Q11H+V264I+A265G+Y267F+Q365CQ11H+V264I+Y267F+F328L+Q365C Q11H+Y267F+Q365C Q11H+V264I+A265G+Q365CQ169*+S170*+A174* Q169*+S170*+R171*+A174*Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473PR116K+S132L+Q136R+R181C+N195F+Q365SR116K+S132P+Q136D+R181Q+N195F+A204V+Q365SR116K+S132P+Q136D+R181Q+N195F+A204V+Q365SR116N+S132A+Q136D+R181K+N195F+Q365S R116N+S132M+Q136R+N195F+Q365SR116N+S132A+Q136D+R181K+N195F+Q365S R116S+S132F+R181N+N195F+Q365SR116S+Y135H+Q136G+R181T+N195F+Q365S R116Y+S132P+Q136N+N195F+Q365SR127H+L235I+Q365S+G435C R127L+M246V+Q365S R127L+Q365C R127L+Q365S R127LR127N+M246L+L275V+Q365S R127N+Q365S R127N+R171L+M246L+L275V+Q365SR127N+R171H+M246L+L275V+Q365S R171H+Q365C R171H+M246L+L275V+Q365SR171H+N302H+Q365S R171H R171H+Q365C R171L+N302H+Q365C R171L+Q365SR171Y++M246L+Q365S R171Y+N306K R181S+G184S V104A+N195F+Q365SV264A+Y267F+V291T+F328L+Q365C V264I+A265G+V291A+F328L+Q365SV264I+A265G+V291A+Q365C V264I+A265G+Y267F+F328L+Q365S+Q395KV264I+A265G+Y267F+F328L+Q365S V264I+A265G+Y267F+V291I+F328L+Q365SV264I+A265G+Y267F V264I+F328L+Q365C V264I+F328L+Q365S V264I+L357FV264I+Q365C V264I+V291I+Q365S V264I+V291T+Q365K V264I+Y267F+F328L+Q365CV264I+Y267F+F328L+Q365K V264I+Y267F+F328L+Q365SV264I+Y267F+Q365K+W408H+V410I V264I+Y267F+Q365K V264I+Y267F+Q365SV264I+Y267F+V291A+F328L+Q365C V264I+Y267F+V291A+F328L+Q365SV264I+Y267F+V291T+F328L+Q365S V264I+A265G+Y267F V264I+F328L+Q365CV264I+Y267F+F328L+Q365K V264I+Y267F+V291T+F328L+Q365SV264T+A265G+Y267F+V291A+F328L+Q365C V264T+A265G+Y267L+F328L+Q365CV264T+V291A+F328L+Q365S V264T+Y267F+Q365S V264T+Y267L+F328L+Q365CV56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6KV56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KW187M+N195F+H210K+Q365SY160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476KY160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G4 76KY160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G4 76KY160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G4 76KY160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476KY160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+N195Y+E212 D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY267F+Q365S Y267F+V291A+F328L+Q365C Y267F+V291A+F328L+Q365S Y267F+Q365SY267F+V291A+F328L+Q365S

Y295F, using SEQ ID NO: 1 for numbering and wherein said variantImprovement Factor (IF) of at least 1.1, preferably at least 1.2, suchas at least 1.3, such as at least 1.4, such as at least 1.5, such as atleast 1.6, such as at least 1.7, such as at least 1.8, such as at least1.9, such as at least 2.0, such as at least 2.2, such as at least 2.4,such as at least 2.6, such as at least 2.8, such as at least 3.0, suchas at least 3.2, such as at least 3.4, such as at least 3.6, such as atleast 3.8, or such as at least 4.0 in Model A detergent compositionand/or in ADW detergent composition with bleach when compared to saidparent alpha-amylase and wherein said parent alpha-amylase has aminoacid sequence as shown in SEQ ID NO: 2.

In one aspect, the variant is selected from a group consisting of:

A60T

Y135H

Q169E

W48Y

A174*

M246L

R90Q

Y203L+V206L

Q169F+S170A

S132D+G133D

N195F+Q365C

N195F+Q365S

N195F+Q365M

R127L+Q365S

R127N+Q365S

R127L+Q365C

N195F+Q365K

I9M+N195F

R171H+Q365C

Y267F+Q365S

F328L+Q365S

R171L+Q365S

N195F+M286L

T134P+N195F

S170*+R171*+A174*

V264I+A265G+Y267F

Q169*+S170*+A174*

M246 L+L275V+Q365S

V264I+V291I+Q365S

V264I+V291T+Q365K

V264I+F328L+Q365C

I9M+N195F+Q365C

Q11H+Y267F+Q365C

I9M+N195F+Q365S

V264I+Y267F+Q365K

R127L+M246V+Q365S

A60S+R171Y+Q365S

R127N+M246T+L275V

V104A+N195F+Q365S

V264I+F328L+Q365S

R171H+N302H+Q365S

V264I+Y267F+Q365S

A174*+N175*+Y178F

V264I+A265G+Q365C

R171L+N302H+Q365C

R171L+M246V+Q365C

R171Y+M246L+Q365S

V264T+Y267F+Q365S

I9M+Y160H+N195F+Q365S

I9M+R127N+N195F+Q365S

R127H+L235I+Q365S+G4350

I9M+R171H+N195F+Q365S

I9M+A186H+N195F+Q365S

I9M+R171L+N195F+Q365S

Q169*+S170*+R171*+A174*

M246L+L275V+Q365S+D406H

I9M+G184K+N195F+Q365S

R127N+M246L+L275V+Q365S

R171H+M246L+L275V+Q365S

R127H+M246I+Q365C+V481A

Y267F+V291A+F328L+Q365C

I9M+A186E+N195F+Q365S

E86L+R171L+N306R+Q365S

V264I+Y267F+F328L+Q365S

I9M+N195F+V291A+Q365S

R127L+L235I+M246I+Q365C

Y267F+V291A+F328L+Q365S

V264I+Y267F+F328L+Q365K

Q11H+V264I+A265G+Q365C

W187M+N195F+H210K+Q365S

R127H+M246T+L275A+Q365S

I9M+A111T+N195F+Q365S

I9M+Y58F+N195F+Q365S

V264T+V291A+F328L+Q365S

V264I+A265G+V291A+Q365C

V264I+A265G+Y267F+Q365C

V264I+Y267F+V291A+F328L+Q365S

R127N+R171H+M246L+L275V+Q365S

V264I+A265G+V291A+F328L+Q365S

R127N+M246I+A265G+Y267H+Q365S

E130I+T134A+R181N+N195F+Q365S

R127N+M246L+L275V+N306R+Q365S

I9M+N195F+V206L+D209N+Q365S

R116S+S132F+R181N+N195F+Q365S

E130T+T134Y+A186K+N195F+Q365S

A37V+V264I+Y267F+F328L+Q365C

Q11H+V264I+Y267F+F328L+Q365C

V264I+Y267F+V291T+F328L+Q365S

Q169*+R171*+Q172*+L173*+A174*

R116Y+S132P+Q136N+N195F+Q365S

N195F+G337D+Q365S+P375K+D377Y

V264I+A265G+Y267F+F328L+Q365S

I9M+N195F+K269Q+A274K+Q365S

E130D+T134Y+R181H+N195F+Q365S

R116N+S132M+Q136R+N195F+Q365S

N195F+G337D+Q365S+P375K+D377N

R127N+R171L+M246L+L275V+Q365S

N195F+G337E+Q365S+P375K+D377N

Q11H+V264I+A265G+Y267F+Q365C

R158Q+Y160N+W187M+N195F+Q365S

V264I+Y267F+Q365K+W408H+V410I

R127N+R171Y+M246L+L275V+Q365S

N54Q+F113T+R171H+N302A+Q365C

V264A+Y267F+V291T+F328L+Q365C

Q169*+S170*+R171*+Q172*+L173*+A174*

R116K+S132L+Q136R+R181C+N195F+Q365S

V264T+A265G+Y267F+V291A+F328L+Q365C

E130N+T134S+R181N+A186K+N195F+Q365S

E130V+T134N+R181Y+A186K+N195F+Q365S

E130D+T134W+R181KA186E+N195F+Q365S

R116N+S132A+Q136D+R181K+N195F+Q365S

E130S+T134E+R181S+A186T+N195F+Q365S

R116C+S132A+Q136S+R181A+N195F+Q365S

R116K+S132P+Q136D+R181Q+N195F+Q365S

R127H+R171H+M246L+L275V+N306R+Q365S

E130L+T134N+R181W+A186T+N195F+Q365S

R116C+S132M+Q136S+R181Q+N195F+Q365S

R116S+Y135H+Q136G+R181T+N195F+Q365S

R116S+S132L+Q136E+R181G+N195F+Q365S

N16E+N195F+G337E+Q365S+P375T+D377H

V264I+A265G+Y267F+V291I+F328L+Q365S

N16E+N195F+G337D+Q365S+P375K+D377N

V264I+A265G+Y267F+F328L+Q365S+Q395K

E130N+T134C+R181Q+A186N+N195F+Q365S

V264I+A265G+Y267F+V291A+F328L+Q365C

W167*+Q169*+S170*+R171*+Q172*+L173*+A174*

W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*

R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S

N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S

E130K+S132T+T134Y+R181T+A186K+N195F+Q365S

E130Y+S132T+T134S+R181E+A186D+N195F+Q365S

A139T+R158S+Y160D+W187M+N195F+H210N+Q365S

I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S

H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A

W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*

H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A

Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A

H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A

H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A

H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A

H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A

H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*-FG109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L

H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A

R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A

H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A

H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A

H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A

H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A

H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A

H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302K+K391A

H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A

H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A

Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K

H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L

H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A

-   -   Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A    -   H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A    -   Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A    -   V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q125G+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311K+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K269M+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+K391A    -   Y160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+N195Y+E212        D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A    -   Y160H+E212        D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K    -   G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A    -   Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A    -   Y160H+N195Q+E212        D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   Y160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A    -   Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A    -   H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A    -   Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F    -   Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A    -   H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A    -   V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K    -   H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K        391A    -   H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A    -   H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A    -   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302        K+S303G+K391A    -   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A    -   H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q1        72        K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W40        8H+R444T+Q445S+Q466S, using SEQ ID NO: 1 for numbering and        wherein said variant Improvement Factor (IF) of at least 1.1,        preferably at least 1.2, such as at least 1.3, such as at least        1.4, such as at least 1.5, such as at least 1.6, such as at        least 1.7, such as at least 1.8, such as at least 1.9, such as        at least 2.0, such as at least 2.2, such as at least 2.4, such        as at least 2.6, such as at least 2.8, such as at least 3.0,        such as at least 3.2, such as at least 3.4, such as at least        3.6, such as at least 3.8, or such as at least 4.0 in Model A        detergent composition when compared to said parent alpha-amylase        and wherein said parent alpha-amylase has amino acid sequence as        shown in SEQ ID NO: 2.

In one aspect, the variant is selected from a group consisting of:

A139T+R158S+Y160D+W187M+N195F+H210N+Q365S A186D+N195F A60S+R171Y+Q365SE130D+T134W+R181K+A186E+N195F+Q365S E130D+T134Y+R181H+N195F+Q365SE130D+T134W+R181K+A186E+N195F+Q365S E130F+T134R+R181S+A186D+N195F+Q365SE130I+T134A+R181N+N195F+Q365S E130I+T134A+R181N+N195F+Q365SE130L+T134N+R181W+A186T+N195F+Q365S E130N+T134C+R181Q+A186N+N195F+Q365SE130S+T134E+R181S+A186T+N195F+Q365S E130T+T134Y+A186K+N195F+Q365SE130V+T134N+R181Y+A186K+N195F+Q365SE130Y+S132T+T134S+R181E+A186D+N195F+Q365SE130Y+S132T+T134S+R181E+A186D+N195F+Q365S E86L+R171L+N306R+Q365SE86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KE86L+R171L+N306R+Q365S F113Q+R116H F113T+R171K+G273V F328L+Q365S

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S

H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391AH1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458KH1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391AH1*+N54S+V56T+G109A+R171H+N195F+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391AH1*+N54S+V56T+G109A+R171H+N195F+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391AI9M+A186E+N195F+Q365S I9M+A186H+N195F+Q365S I9M+G184K+N195F+Q365SI9M+N195F+K269Q+A274K+Q365S I9M+N195F+N270G+Q365S I9M+N195F+N270P+Q365SI9M+N195F+Q365C I9M+N195F+V206L+D209N+Q365S I9M+N195F+V291A+Q365SI9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365SI9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S I9M+R171L+N195F+Q365SI9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KI9M+Y160H+N195F+Q365S I9M+A186E+N195F+Q365S I9M+A186H+N195F+Q365SI9M+G184K+N195F+Q365S

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S

I9M+N195F+Q365CI9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458KI9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365SI9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S I9M+R171L+N195F+Q365SI9M+Y160H+N195F+Q365S M202L M246L M246L+L275V+Q365S+D406HM246L+L275V+Q365S M246L+L275V+Q365S+D406HN16E+N195F+G337D+Q365S+P375K+D377N N16E+N195F+G337E+Q365S+P375T+D377HN16E+N195F+G337E+Q365S+P375T+D377HN16Y+Y160H+N195F+L275I+S304G+G337E+Q365SN16Y+Y160H+N195F+L275I+S304G+G337E+Q365S N195F+G337D+Q365S+P375K+D377NN195F+G337D+Q365S+P375K+D377Y N195F+G337E+Q365S+P375K+D377NN195F+G337D+Q365S+P375K+D377Y N195F+G337E+Q365S+P375K+D377N N195F+Q365SN54Q+F113T+R171H+N302A+Q365C Q11H+V264I+A265G+Q365CQ11H+V264I+A265G+Y267F+Q365C Q11H+V264I+Y267F+F328L+Q365CQ11H+Y267F+Q365C Q11H+V264I+A265G+Q365C Q169*+S170*+A174*Q169*+S170*+R171*+A174* Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473PR116K+S132L+Q136R+R181C+N195F+Q365SR116K+S132P+Q136D+R181Q+N195F+A204V+Q365SR116K+S132P+Q136D+R181Q+N195F+A204V+Q365SR116N+S132A+Q136D+R181K+N195F+Q365S R116N+S132M+Q136R+N195F+Q365SR116N+S132A+Q136D+R181K+N195F+Q365S R116S+S132F+R181N+N195F+Q365SR116S+Y135H+Q136G+R181T+N195F+Q365S R116Y+S132P+Q136N+N195F+Q365SR127H+L235I+Q365S+G435C R127L R127L+M246V+Q365S R127L+Q365C R127L+Q365SR127N+M246L+L275V+Q365S R127N+Q365S R127N+R171L+M246L+L275V+Q365SR127N+R171H+M246L+L275V+Q365S R171H R171H+Q365C R171H+M246L+L275V+Q365SR171H+N302H+Q365S R171H+Q365C R171L+N302H+Q365C R171L+Q365SR171Y++M246L+Q365S R171Y+N306K

*

R181S+G184S V104A+N195F+Q365S V264A+Y267F+V291T+F328L+Q365CV264I+A265G+V291A+F328L+Q365S V264I+A265G+V291A+Q365C V264I+A265G+Y267FV264I+A265G+Y267F+F328L+Q365S V264I+A265G+Y267F+F328L+Q365S+Q395KV264I+A265G+Y267F+V291I+F328L+Q365S V264I+F328L+Q365C V264I+F328L+Q365SV264I+L357F V264I+Q365C V264I+V291I+Q365S V264I+V291T+Q365KV264I+Y267F+F328L+Q365C V264I+Y267F+F328L+Q365K V264I+Y267F+F328L+Q365SV264I+Y267F+Q365K V264I+Y267F+Q365K+W408H+V410I V264I+Y267F+Q365SV264I+Y267F+V291A+F328L+Q365C V264I+Y267F+V291A+F328L+Q365SV264I+Y267F+V291T+F328L+Q365S V264I+A265G+Y267F V264I+F328L+Q365CV264I+Y267F+F328L+Q365K V264I+Y267F+V291T+F328L+Q365SV264T+A265G+Y267F+V291A+F328L+Q365C V264T+A265G+Y267L+F328L+Q365CV264T+V291A+F328L+Q365S V264T+Y267F+Q365S V264T+Y267L+F328L+Q365CW187M+N195F+H210K+Q365SY160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476KY160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476KY160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476KY267F+Q365S Y267F+V291A+F328L+Q365C Y267F+V291A+F328L+Q365S Y267F+Q365SY267F+V291A+F328L+Q365S

Y295F, using SEQ ID NO: 1 for numbering and wherein said variantImprovement Factor (IF) of at least 1.1, preferably at least 1.2, suchas at least 1.3, such as at least 1.4, such as at least 1.5, such as atleast 1.6, such as at least 1.7, such as at least 1.8, such as at least1.9, such as at least 2.0, such as at least 2.2, such as at least 2.4,such as at least 2.6, such as at least 2.8, such as at least 3.0, suchas at least 3.2, such as at least 3.4, such as at least 3.6, such as atleast 3.8, or such as at least 4.0 in ADW detergent with bleach whencompared to said parent alpha-amylase and wherein said parentalpha-amylase has amino acid sequence as shown in SEQ ID NO: 2.In one aspect, the variant is selected from the group consisting of:

A60T, Y135H, Q169E, W48Y, A174*, M246L, R90Q, Y203L+V206L, Q169F+S170A,S132D+G133D, N195F+Q365C, N195F+Q365S, N195F+Q365M, N195F+Q365K,R127L+Q365S, R127N+Q365S, R127L+Q365C, R171L+Q365S, R171H+Q365C,Y267F+Q365S, F328L+Q365S, I9M+N195F, N195F+M286L, T134P+N195F,S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,M246L+L275V+Q365S, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C, I9M+N195F+Q365S,V264I+Y267F+Q365K, R127L+M246V+Q365S, A60S+R171Y+Q365S,R127N+M246T+L275V, V104A+N195F+Q365S, V264I+F328L+Q365S,R171H+N302H+Q365S, V264I+Y267F+Q365S, A174*+N175*+Y178F,V264I+A265G+Q365C, R171L+N302H+Q365C, R171L+M246V+Q365C,R171Y+M246L+Q365S, V264T+Y267F+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, I9M+R127N+N195F+Q365S,R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, I9M+R171H+N195F+Q365S, W187M+N195F+H210K+Q365S,R127H+M246T+L275A+Q365S, I9M+A111T+N195F+Q365S, I9M+Y58F+N195F+Q365S,V264T+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F+Q365C, V264I+Y267F+V291A+F328L+Q365S,R127N+R171H+M246L+L275V+Q365S, V264I+A265G+V291A+F328L+Q365S,R127N+M246I+A265G+Y267H+Q365S, E130I+T134A+R181N+N195F+Q365S,R127N+M246L+L275V+N306R+Q365S, I9M+N195F+V206L+D209N+Q365S,R116S+S132F+R181N+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,A37V+V264I+Y267F+F328L+Q365C, Q11H+V264I+Y267F+F328L+Q365C,V264I+Y267F+V291T+F328L+Q365S, Q169*+R171*+Q172*+L173*+A174*,R116Y+S132P+Q136N+N195F+Q365S, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+Y267F+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,E130D+T134Y+R181H+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N195F+G337D+Q365S+P375K+D377N, R127N+R171L+M246L+L275V+Q365S,N195F+G337E+Q365S+P375K+D377N, Q11H+V264I+A265G+Y267F+Q365C,R158Q+Y160N+W187M+N195F+Q365S, V264I+Y267F+Q365K+W408H+V410I,R127N+R171Y+M246L+L275V+Q365S, N54Q+F113T+R171H+N302A+Q365C,V264A+Y267F+V291T+F328L+Q365C, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R116C+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,V264I+A265G+Y267F+V291I+F328L+Q365S, N16E+N195F+G337D+Q365S+P375K+D377N,V264I+A265G+Y267F+F328L+Q365S+Q395K,E130N+T1340+R181Q+A186N+N195F+Q365S,V264I+A265G+Y267F+V291A+F328L+Q365C,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302 K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+FG109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q125G+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K269M+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+K391A,Y160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Y+E212 D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A,Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A,Y160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A,H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6K,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A,H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S, using SEQ ID NO: 1 for numbering and wherein saidvariant has an improved relative specific activity of at least 1.1 inModel A detergent composition as a measure of wash performance comparedto parent alpha-amylase of SEQ ID NO: 2.

In one aspect, the variant is selected from the group consisting of:

M246L, N195F+Q365C, N195F+Q365S, N195F+Q365M, N195F+Q365K, R127L+Q365S,R127N+Q365S, R127L+Q365C, R171H+Q365C, Y267F+Q365S, F328L+Q365S,I9M+N195F, S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,M246L+L275V+Q365S, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C, I9M+N195F+Q365S,V264I+Y267F+Q365K, R127L+M246V+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, I9M+R127N+N195F+Q365S,R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, V264I+Y267F+V291A+F328L+Q365S,V264I+A265G+V291A+F328L+Q365S, R127N+M246I+A265G+Y267H+Q365S,E130I+T134A+R181N+N195F+Q365S, R127N+M246L+L275V+N306R+Q365S,I9M+N195F+V206L+D209N+Q365S, R116S+S132F+R181N+N195F+Q365S,E130T+T134Y+A186K+N195F+Q365S, A37V+V264I+Y267F+F328L+Q365C,Q11H+V264I+Y267F+F328L+Q365C, V264I+Y267F+V291T+F328L+Q365S,Q169*+R171*+Q172*+L173*+A174*, R116Y+S132P+Q136N+N195F+Q365S,N195F+G337D+Q365S+P375K+D377Y, V264I+A265G+Y267F+F328L+Q365S,I9M+N195F+K269Q+A274K+Q365S, E130D+T134Y+R181H+N195F+Q365S,R116N+S132M+Q136R+N195F+Q365S, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R116C+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302 K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A, using SEQ ID NO: 1 for numbering and wherein said variant has animproved relative specific activity of at least 1.5 in Model A detergentcomposition as measure of wash performance compared to parentalpha-amylase of SEQ ID NO: 2.

In one aspect, variant is selected from the group consisting of:

N195F+Q365C, N195F+Q365S, N195F+Q365M, R127L+Q365S, R127N+Q365S,S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,V264I+V291I+Q365S, V264I+V291T+Q365K, V264I+F328L+Q365C,I9M+Y160H+N195F+Q365S, I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G4350,I9M+R171H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S,Q169*+S170*+R171*+A174*, M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S,I9M+R127N+N195F+Q365S, R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, V264I+Y267F+V291A+F328L+Q365S,V264I+A265G+V291A+F328L+Q365S, R127N+M246I+A265G+Y267H+Q365S,E130I+T134A+R181N+N195F+Q365S, R127N+M246L+L275V+N306R+Q365S,I9M+N195F+V206L+D209N+Q365S, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A,

H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has animproved relative specific activity of at least 2.0 in Model A detergentcomposition as a measure of wash performance compared to parentalpha-amylase of SEQ ID NO: 2.

In one aspect, variant is selected from the group consisting of:

N195F+Q365C, S170*+R171*+A174*, I9M+Y160H+N195F+Q365S,R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, Q169*+5170*+R171*+Q172*+L173*+A174*,W167*+Q169*+5170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,

Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an improvedrelative specific activity of at least 2.5 in Model A detergentcomposition as a measure of wash performance compared to parentalpha-amylase of SEQ ID NO: 2.

In one embodiment, the variant has an improved wash performance, theenhanced wash performance corresponding to an Improvement Factor (IF) inADW detergent with bleach is assessed using an Automatic MechanicalStress Assay (AMSA) of at least 1.1, preferably at least 1.2, such as atleast 1.3, such as at least 1.4, such as at least 1.5, such as at least1.6, such as at least 1.7, such as at least 1.8, such as at least 1.9,such as at least 2.0, such as at least 2.2, such as at least 2.4, suchas at least 2.6, such as at least 2.8, such as at least 3.0, such as atleast 3.2, such as at least 3.4, such as at least 3.6, such as at least3.8, or such as at least 4.0 when compared to said parent alpha-amylaseand wherein said parent alpha-amylase has amino acid sequence as shownin SEQ ID NO: 2.

In one aspect, the variant is selected from the group consisting of:

Y295F, M246L, M202L, R181S+G184S, F113Q+R116H, V264I+L357F, Y267F+Q365S,R171H, R171Y+N306K, R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S,R171L+N302H+Q365C, R171Y++M246L+Q365S, F328L+Q365S, R127L, R127N+Q365S,R127L+Q365C, R127L+Q365S, R127L+M246V+Q365S, A186D+N195F, V264I+Q365C,I9M+N195F+Q365C, V264I+A265G+Y267F, V264I+V291T+Q365K, A60S+R171Y+Q365S,V264I+Y267F+Q365K, V264I+F328L+Q365C, M246L+L275V+Q365S,V264T+Y267F+Q365S, V264I+F328L+Q365S, Q11H+Y267F+Q365C,V104A+N195F+Q365S, V264I+V291I+Q365S, V264I+Y267F+Q365S,F113T+R171K+G273V, Q169*+S170*+A174*, I9M+R171H+N195F+Q365S,I9M+R127N+N195F+Q365S, E86L+R171L+N306R+Q365S, I9M+A186E+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S, R171H+M246L+L275V+Q365S,I9M+N195F+V291A+Q365S, I9M+G184K+N195F+Q365S, M246L+L275V+Q365S+D406H,Y267F+V291A+F328L+Q365S, Y267F+V291A+F328L+Q365C,V264I+Y267F+F328L+Q365K, I9M+R171L+N195F+Q365S, Q11H+V264I+A265G+Q365C,V264T+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365S,R127N+M246L+L275V+Q365S, I9M+N195F+N270G+Q365S, I9M+N195F+N270P+Q365S,V264T+Y267L+F328L+Q365C, V264I+A265G+V291A+Q365C,W187M+N195F+H210K+Q365S, V264I+Y267F+F328L+Q365C,Q169*+S170*+R171*+A174*, 19M+N195F+V206L+D209N+Q365S,N195F+G337D+Q365S+P375K+D377N, V264I+Y267F+V291T+F328L+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+V291A+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,V264I+Y267F+Q365K+W408H+V410I, E130D+T134Y+R181H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, E130I+T134A+R181N+N195F+Q365S,R116Y+S132P+Q136N+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,V264I+A265G+Y267F+F328L+Q365S, Q11H+V264I+Y267F+F328L+Q365C,Q11H+V264I+A265G+Y267F+Q365C, E130T+T134Y+A186K+N195F+Q365S,R116S+S132F+R181N+N195F+Q365S, V264A+Y267F+V291T+F328L+Q365C,N54Q+F113T+R171H+N302A+Q365C, R127N+R171L+M246L+L275V+Q365S,V264T+A265G+Y267L+F328L+Q365C, V264I+Y267F+V291A+F328L+Q365C,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130V+T134N+R181Y+A186K+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,V264I+A265G+Y267F+F328L+Q365S+Q395K, N16E+N195F+G337D+Q365S+P375K+D377N,E130N+T134C+R181Q+A186N+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116K+S132L+Q136R+R181C+N195F+Q365S,V264I+A265G+Y267F+V291I+F328L+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391A,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 1.1 in ADW detergent compositionwith bleach assessed using AMSA.

In one aspect, the variant is selected from the group consisting of:

Y295F, M246L, M202L, R181S+G184S, F113Q+R116H, Y267F+Q365S, R171H,R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S, R171L+N302H+Q365C,R171Y++M246L+Q365S, F328L+Q365S, I9M+N195F+Q365C, V264I+A265G+Y267F,V264I+V291T+Q365K, A60S+R171Y+Q365S, V264I+Y267F+Q365K,V264I+F328L+Q365C, M246L+L275V+Q365S, V264T+Y267F+Q365S,V264I+F328L+Q365S, Q11H+Y267F+Q365C, I9M+R171H+N195F+Q365S,I9M+R127N+N195F+Q365S, E86L+R171L+N306R+Q365S, I9M+A186E+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+N195F+V291A+Q365S,I9M+G184K+N195F+Q365S, M246L+L275V+Q365S+D406H, Y267F+V291A+F328L+Q365S,Y267F+V291A+F328L+Q365C, V264I+Y267F+F328L+Q365K, I9M+R171L+N195F+Q365S,Q11H+V264I+A265G+Q365C, I9M+N195F+V206L+D209N+Q365S,N195F+G337D+Q365S+P375K+D377N, V264I+Y267F+V291T+F328L+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+V291A+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,V264I+Y267F+Q365K+W408H+V410I, E130D+T134Y+R181H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, E130I+T134A+R181N+N195F+Q365S,R116Y+S132P+Q136N+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 1.5 in ADW detergent compositionwith bleach assessed using AMSA.

In one aspect, the variant is selected from the group consisting of:

M202L, R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S, R171L+N302H+Q365C,I9M+N195F+Q365C, V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,I9M+A186E+N195F+Q365S, I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S,I9M+N195F+V206L+D209N+Q365S, N195F+G337D+Q365S+P375K+D377N,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 2.0 in ADW detergent compositionwith bleach assessed using AMSA.

In one aspect, the variant is selected from the group consisting of:

M202L, R171H+Q365C, I9M+N195F+Q365C,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,

Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least 2.5 in ADW detergent composition with bleachassessed using AMSA.

In one aspect, the variant is selected from the group consisting of:

H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥2.5 in ADW detergent composition with bleachassessed using AMSA.

In one aspect, the variant is selected from the group consisting of:

V264I+A265G+Y267F, R171L+Q365S, Y267F+Q365S, R127L+Q365S,V264I+F328L+Q365C, M246L+L275V+Q365S+D406H, Q11H+V264I+A265G+Q365C,R127N+R171H+M246L+L275V+Q365S, V264I+Y267F+V291T+F328L+Q365S,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,R127H+L235I+Q365S+G4350, N195F+Q365S, I9M+N195F+Q365C,I9M+R127N+N195F+Q365S, I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S,I9M+R171H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+G184K+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,E130I+T134A+R181N+N195F+Q365S, R116N+S132A+Q136D+R181K+N195F+Q365S,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, Y160H+M202 L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391AH1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202 L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 1.1 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

V264I+A265G+Y267F, R127N+R171H+M246L+L275V+Q365S,V264I+Y267F+V291T+F328L+Q365S, I9M+N195F+Q365C, I9M+R127N+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N, N16E+N195F+G337E+Q365S+P375T+D377H,E130D+T134W+R181K+A186E+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 1.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 2.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least 2.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202 L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.

In one aspect, the variant is selected from the group consisting of:

Y295F, M246L, N195F+Q365S, R171H+Q365C, V264I+A265G+Y267F,E86L+R171L+N306R+Q365S, V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+

M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥1.1 in ADW detergent composition with bleachassessed using a full scale automatic dishwash (ADW) at 45° C.

In one aspect, the variant is selected from the group consisting of:

V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,N195F+Q365S, I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+

M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥1.2 in ADW detergent composition with bleachassessed using a full scale automatic dishwash (ADW) at 45° C.

In one aspect, the variant is selected from the group consisting of:

N195F+Q365S, V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+

M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥1.3 in ADW detergent composition with bleachassessed using a full scale automatic dishwash (ADW) at 45° C.

In one aspect, the variant is selected from the group consisting of:

V264I+A265G+Y267F V264I+Y267F+V291T+F328L+Q365S I9M+R171L+N195F+Q365SH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391AH1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+

M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥1.5 in ADW detergent composition with bleachassessed using a full scale automatic dishwash (ADW) at 45° C.

In one aspect, the variant is selected from the group consisting of:

H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥2.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.

In one aspect, the variant is selected from the group consisting of:

H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.

The variants according to the present invention may further comprise oneor more additional alterations than those described above at one or more(e.g., several) other positions.

The amino acid changes may be of a minor nature, that is conservativeamino acid substitutions or insertions that do not significantly affectthe folding and/or activity of the protein; small deletions, typicallyof 1-30 amino acids; small amino- or carboxyl-terminal extensions, suchas an amino-terminal methionine residue; a small linker peptide of up to20-25 residues; or a small extension that facilitates purification bychanging net charge or another function, such as a poly-histidine tract,an antigenic epitope or a binding domain.

Examples of conservative substitutions are within the groups of basicamino acids (arginine, lysine and histidine), acidic amino acids(glutamic acid and aspartic acid), polar amino acids (glutamine andasparagine), hydrophobic amino acids (leucine, isoleucine and valine),aromatic amino acids (phenylalanine, tryptophan and tyrosine), and smallamino acids (glycine, alanine, serine, threonine and methionine). Aminoacid substitutions that do not generally alter specific activity areknown in the art and are described, for example, by H. Neurath and R. L.Hill, 1979, In, The Proteins, Academic Press, New York. Commonsubstitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr,Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile,Leu/Val, Ala/Glu, and Asp/Gly.

Alternatively, the amino acid changes are of such a nature that thephysico-chemical properties of the polypeptides are altered. Forexample, amino acid changes may improve the thermal stability of thepolypeptide, alter the substrate specificity, change the pH optimum, andthe like.

Essential amino acids in a polypeptide may be identified according toprocedures known in the art, such as site-directed mutagenesis oralanine-scanning mutagenesis (Cunningham and Wells, 1989, Science 244:1081-1085). In the latter technique, single alanine mutations areintroduced at every residue in the molecule, and the resultant mutantmolecules are tested for alpha-amylase activity to identify amino acidresidues that are critical to the activity of the molecule. See also,Hilton et al., 1996, J. Biol. Chem. 271: 4699-4708. The active site ofthe enzyme or other biological interaction can also be determined byphysical analysis of structure, as determined by such techniques asnuclear magnetic resonance, crystallography, electron diffraction, orphotoaffinity labeling, in conjunction with mutation of putative contactsite amino acids. See, for example, de Vos et al., 1992, Science 255:306-312; Smith et al., 1992, J. Mol. Biol. 224: 899-904; Wlodaver etal., 1992, FEBS Led. 309: 59-64. The identity of essential amino acidscan also be inferred from an alignment with a related polypeptide.

The variants according to the present invention may consist of 400 to485 amino acids, e.g., 410 to 485, 425 to 485, and 440 to 485 aminoacids.

Parent Alpha-Amylases

The parent alpha-amylase may be a polypeptide with at least 60% sequenceidentity with any one of the polypeptides of SEQ ID Nos: 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or 17.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 1 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 1.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 1. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 1. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 1.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 2 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 2.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 2. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 2. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 2.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 3 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 3.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 3. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 3. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 3.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 4 of at least 60% e.g., at least 65%, at least 70%, atleast 80%, at least 85%, at least 87%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, or 100%, which havealpha-amylase activity. In one embodiment, the amino acid sequence ofthe parent differs by no more than ten amino acids, e.g., by five aminoacids, by four amino acids, by three amino acids, by two amino acids,and by one amino acid from the polypeptide of SEQ ID NO: 4.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 4. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 4. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 4.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 5 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 5.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 5. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 5. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 5.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 6 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 6.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 6. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 6. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 6.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 7 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 7.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 7. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 7. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 7.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 8 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 8.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 8. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 8. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 8.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 9 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 9.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 9. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 9. In another embodiment, the parent is anallelic variant of the polypeptide of SEQ ID NO: 9.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 10 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 10.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 10. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 10. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO:10.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 11 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 11.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 11. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 11. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 11.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 12 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 12.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 12. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 12. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 12.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 13 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 13.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 13. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 13. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 13.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 14 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 14.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 14. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 14. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 14.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 15 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 15.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 15. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 15. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 15.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 16 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 16.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 16. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 16. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 16.

In one embodiment, the parent has a sequence identity to the polypeptideof SEQ ID NO: 17 of at least 60% e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 87%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100%, which have alpha-amylase activity. In one embodiment, the aminoacid sequence of the parent differs by no more than ten amino acids,e.g., by five amino acids, by four amino acids, by three amino acids, bytwo amino acids, and by one amino acid from the polypeptide of SEQ IDNO: 17.

The parent preferably comprises or consists of the amino acid sequenceof SEQ ID NO: 17. In one embodiment the parent comprises or consists ofthe polypeptide of SEQ ID NO: 17. In another embodiment, the parent isan allelic variant of the polypeptide of SEQ ID NO: 17.

The amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ IDNO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or an activefragment thereof, may be used to design nucleic acid probes to identifyand clone DNA encoding a parent from strains of different genera orspecies according to methods well known in the art. In particular, suchprobes can be used for hybridization with the genomic or cDNA of thegenus or species of interest, following standard Southern blottingprocedures, in order to identify and isolate the corresponding genetherein. Such probes can be considerably shorter than the entiresequence, but should be at least 14, e.g., at least 25, at least 35, orat least 70 nucleotides in length. Preferably, the nucleic acid probe isat least 100 nucleotides in length or at least 200 nucleotides inlength., at least 300 nucleotides, at least 400 nucleotides, at least500 nucleotides, at least 600 nucleotides, at least 700 nucleotides, atleast 800 nucleotides, or at least 900 nucleotides in length. Both DNAand RNA probes can be used. The probes are typically labeled fordetecting the corresponding gene (for example, with ³²P, ³H, ³⁵S,biotin, or avidin). Such probes are encompassed by the presentinvention.

A genomic DNA or cDNA library prepared from such other organisms may bescreened for DNA that hybridizes with the probes described above andencodes a parent. Genomic or other DNA from such other organisms may beseparated by agarose or polyacrylamide gel electrophoresis, or otherseparation techniques. DNA from the libraries or the separated DNA maybe transferred to and immobilized on nitrocellulose or other suitablecarrier material, which is used in a Southern blot.

For purposes of the present invention, hybridization indicates that thepolynucleotide hybridizes to a labeled nucleotide probe corresponding toa polynucleotide encoding SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ IDNO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or a subsequencethereof, under low to very high stringency conditions. Molecules towhich the probe hybridizes can be detected using, for example, X-rayfilm or any other detection means known in the art.

In one aspect, the nucleic acid probe is a polynucleotide that encodesthe polypeptide of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO:4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9,SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO:14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, or an active fragmentthereof.

For long probes of at least 100 nucleotides in length, very low to veryhigh stringency conditions are defined as prehybridization andhybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml shearedand denatured salmon sperm DNA, and either 25% formamide for very lowand low stringencies, 35% formamide for medium and medium-highstringencies, or 50% formamide for high and very high stringencies,following standard Southern blotting procedures for 12 to 24 hoursoptimally. The carrier material is finally washed three times each for15 minutes using 2×SSC, 0.2% SDS at 45° C. (very low stringency), 50° C.(low stringency), 55° C. (medium stringency), 60° C. (medium-highstringency), 65° C. (high stringency), or 70° C. (very high stringency).

For short probes that are about 15 nucleotides to about 70 nucleotidesin length, stringency conditions are defined as prehybridization andhybridization at about 5° C. to about 10° C. below the calculated T_(m)using the calculation according to Bolton and McCarthy (1962, Proc.Natl. Acad. Sci. USA 48: 1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6mM EDTA, 0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA perml following standard Southern blotting procedures for 12 to 24 hoursoptimally. The carrier material is finally washed once in 6×SCC plus0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5°C. to 10° C. below the calculated T_(m). The parent may be obtained frommicroorganisms of any genus. For purposes of the present invention, theterm “obtained from” as used herein in connection with a given sourceshall mean that the parent encoded by a polynucleotide is produced bythe source or by a cell in which the polynucleotide from the source hasbeen inserted. In one aspect, the parent is secreted extracellularly.

The parent may be a bacterial alpha-amylase. For example, the parent maybe a gram-positive bacterial polypeptide such as a Bacillus,Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus,Oceanobacillus, Staphylococcus, Streptococcus, or Streptomycesalpha-amylase, or a gram-negative bacterial polypeptide such as aCampylobacter, E. coli, Flavobacterium, Fusobacterium, Helicobacter,Ilyobacter, Neisseria, Pseudomonas, Salmonella, or Ureaplasmaalpha-amylase.

In one aspect, the parent is a Bacillus alkalophilus, Bacillusamyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillusclausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacilluslentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus,Bacillus stearothermophilus, Bacillus subtilis, or Bacillusthuringiensis alpha-amylase.

In another aspect, the parent is a Streptococcus equisimilis,Streptococcus pyogenes, Streptococcus uberis, or Streptococcus equisubsp. Zooepidemicus alpha-amylase.

In another aspect, the parent is a Streptomyces achromogenes,Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces griseus,or Streptomyces lividans alpha-amylase.

The parent may be a fungal alpha-amylase. For example, the parent may bea yeast alpha-amylase such as a Candida, Kluyveromyces, Pichia,Saccharomyces, Schizosaccharomyces, or Yarrowia alpha-amylase. Forexample, the parent may be a filamentous fungal alpha-amylase such as anAcremonium, Agaricus, Alternaria, Aspergillus, Aureobasidium,Botryosphaeria, Ceriporiopsis, Chaetomidium, Chrysosporium, Claviceps,Cochliobolus, Coprinopsis, Coptotermes, Corynascus, Cryphonectria,Cryptococcus, Diplodia, Exidia, Filibasidium, Fusarium, Gibberella,Holomastigotoides, Humicola, Irpex, Lentinula, Leptospaeria,Magnaporthe, Melanocarpus, Meripilus, Mucor, Myceliophthora,Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete,Piromyces, Poitrasia, Pseudoplectania, Pseudotrichonympha, Rhizomucor,Schizophyllum, Scytalidium, Talaromyces, Thermoascus, Thielavia,Tolypocladium, Trichoderma, Trichophaea, Verticillium, Volvariella, orXylaria alpha-amylase.

In another aspect, the parent is a Saccharomyces carlsbergensis,Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomycesdouglasii, Saccharomyces kluyveri, Saccharomyces norbensis, orSaccharomyces oviformis alpha-amylase.

In another aspect, the parent is an Acremonium cellulolyticus,Aspergillus aculeatus, Aspergillus awamori, Aspergillus foetidus,Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans,Aspergillus niger, Aspergillus oryzae, Chrysosporium inops,Chrysosporium keratinophilum, Chrysosporium lucknowense, Chrysosporiummerdarium, Chrysosporium pannicola, Chrysosporium queenslandicum,Chrysosporium tropicum, Chrysosporium zonatum, Fusarium bactridioides,Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusariumgraminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi,Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusariumsambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusariumsulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusariumvenenatum, Humicola grisea, Humicola insolens, Humicola lanuginosa,lrpex lacteus, Mucor miehei, Myceliophthora thermophila, Neurosporacrassa, Penicillium funiculosum, Penicillium purpurogenum, Phanerochaetechrysosporium, Thielavia achromatica, Thielavia albomyces, Thielaviaalbopilosa, Thielavia australeinsis, Thielavia fimeti, Thielaviamicrospora, Thielavia ovispora, Thielavia peruviana, Thielavia setosa,Thielavia spededonium, Thielavia subthermophila, Thielavia terrestris,Trichoderma harzianum, Trichoderma koningii, Trichodermalongibrachiatum, Trichoderma reesei, or Trichoderma viridealpha-amylase.

In another aspect, the parent is a Bacillus sp. alpha-amylase, e.g., thealpha-amylase of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4,SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9,SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO:14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17.

It will be understood that for the aforementioned species, the inventionencompasses both the perfect and imperfect states, and other taxonomicequivalents, e.g., anamorphs, regardless of the species name by whichthey are known. Those skilled in the art will readily recognize theidentity of appropriate equivalents.

Strains of these species are readily accessible to the public in anumber of culture collections, such as the American Type CultureCollection (ATCC), Deutsche Sammlung von Mikroorganismen andZellkulturen GmbH (DSM), Centraalbureau Voor Schimmelcultures (CBS), andAgricultural Research Service Patent Culture Collection, NorthernRegional Research Center (NRRL).

The parent may be identified and obtained from other sources includingmicroorganisms isolated from nature (e.g., soil, composts, water, etc.)or DNA samples obtained directly from natural materials (e.g., soil,composts, water, etc,) using the above-mentioned probes. Techniques forisolating microorganisms and DNA directly from natural habitats are wellknown in the art. The polynucleotide encoding a parent may then bederived by similarly screening a genomic or cDNA library of anothermicroorganism or mixed DNA sample. Once a polynucleotide encoding aparent has been detected with a probe(s), the polynucleotide may beisolated or cloned by utilizing techniques that are known to those ofordinary skill in the art (see, e.g., Sambrook et al., 1989, supra).

The parent may be a hybrid polypeptide in which a portion of onepolypeptide is fused at the N-terminus or the C-terminus of a portion ofanother polypeptide.

The parent may also be a fused polypeptide or cleavable fusionpolypeptide in which one polypeptide is fused at the N-terminus or theC-terminus of another polypeptide. A fused polypeptide is produced byfusing a polynucleotide encoding one polypeptide to a polynucleotideencoding another polypeptide. Techniques for producing fusionpolypeptides are known in the art, and include ligating the codingsequences encoding the polypeptides so that they are in frame and thatexpression of the fused polypeptide is under control of the samepromoter(s) and terminator. Fusion proteins may also be constructedusing intein technology in which fusions are createdpost-translationally (Cooper et al., 1993, EMBO J. 12: 2575-2583; Dawsonet al., 1994, Science 266: 776-779).

A fusion polypeptide can further comprise a cleavage site between thetwo polypeptides. Upon secretion of the fusion protein, the site iscleaved releasing the two polypeptides. Examples of cleavage sitesinclude, but are not limited to, the sites disclosed in Martin et al.,2003, J. Ind. Microbiol. Biotechnol. 3: 568-576; Svetina et al., 2000,J. Biotechnol. 76: 245-251; Rasmussen-Wilson et al., 1997, Appl.Environ. Microbiol. 63: 3488-3493; Ward et al., 1995, Biotechnology 13:498-503; and Contreras et al., 1991, Biotechnology 9: 378-381; Eaton etal., 1986, Biochemistry 25: 505-512; Collins-Racie et al., 1995,Biotechnology 13: 982-987; Carter et al., 1989, Proteins: Structure,Function, and Genetics 6: 240-248; and Stevens, 2003, Drug DiscoveryWorld 4: 35-48.

Polynucleotides

The present invention also relates to polynucleotides encoding a variantof the present invention. Thus, in particular, the present inventionrelates to a polynucleotide encoding a variant comprising a modificationat one or more positions corresponding to positions: H1, H2, G7, I9,Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81,E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481, using SEQ ID NO: 1 for numbering.

The term “polynucleotides encoding” as used herein, refers to apolynucleotide that encodes a polypeptide having alpha-amylase havingalpha-amylase activity. In one aspect, the polypeptide coding sequenceis the nucleotide sequence set forth in SEQ ID NO: 18.

In one embodiment, the polynucleotide encoding a variant according tothe present invention as at least 60%, such as at least 65%, such as atleast 70%, such as at least 75%, such as at least 80%, such as at least85%, such as at least 90%, such as at least 95%, such as at least 96%,such as at least 97%, such as at least 98%, such as at least 99% butless than 100% sequence identity to the polynucleotide of SEQ ID NO: 18.

Nucleic Acid Constructs

The present invention also relates to nucleic acid constructs comprisinga polynucleotide encoding a variant of the present invention operablylinked to one or more control sequences that direct the expression ofthe coding sequence in a suitable host cell under conditions compatiblewith the control sequences. Thus, in particular, the present inventionrelates to a nucleic acid construct comprising a polynucleotide encodinga variant comprising a modification at one or more positionscorresponding to positions H1, H2, G7, I9, Q11, N16, N19, N25, A37, W43,W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90, Q98, V104, G109,A111, F113, R116, Q118, Q125, R127, E130, S132, G133, T134, Y135, Q136,A139, G142, N144, G149, R158, Y160, D163, W167, Q169, S170, R171, Q172,L173, A174, N175, R176, Y178, R181, G182, A186, W187, N195, M202, Y203,A204, V206, D209, H210, E212, T227, L235, V238, M246, Q256, K259, V264,A265, E266, Y267, K269, N270, L272, G273, A274, L275, E276, W284, M286,V291, L293, Y295, Y298, Q299, N302, S303, S304, N306, R310, N311, N314,G315, L317, Q319, R320, S323, F328, G337, A339, Q345, D357, Q365, P377,D375, Q385, K391, Q395, R400, D406, W408, V410, S431, G435, W439, R444,Q445, R458, N465, Q466, W469, F473, G476 and V481A, using SEQ ID NO: 1for numbering.

The term “nucleic acid construct” as used herein, refers to a nucleicacid molecule, either single- or double-stranded, which is isolated froma naturally occurring gene or is modified to contain segments of nucleicacids in a manner that would not otherwise exist in nature or which issynthetic, which comprises one or more control sequences.

The term “operably linked” as used herein, refers to a configuration inwhich a control sequence is placed at an appropriate position relativeto the coding sequence of a polynucleotide such that the controlsequence directs expression of the coding sequence.

The polynucleotide may be manipulated in a variety of ways to providefor expression of a variant. Manipulation of the polynucleotide prior toits insertion into a vector may be desirable or necessary depending onthe expression vector. The techniques for modifying polynucleotidesutilizing recombinant DNA methods are well known in the art.

The control sequence may be a promoter, a polynucleotide which isrecognized by a host cell for expression of the polynucleotide. Thepromoter comprises transcriptional control sequences that mediate theexpression of the variant. The promoter may be any polynucleotide thatshows transcriptional activity in the host cell including mutant,truncated, and hybrid promoters, and may be obtained from genes encodingextracellular or intracellular polypeptides either homologous orheterologous to the host cell.

Expression Vectors

The present invention also relates to recombinant expression vectorscomprising a polynucleotide encoding a variant of the present invention,a promoter, and transcriptional and translational stop signals. Thus,the present invention relates to an expression vector, optionallyrecombinant, comprising a polynucleotide encoding a variant comprising amodification at one or more positions corresponding to positions H1, H2,G7, I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63,T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481, using SEQ ID NO: 1 for numbering, a promotor, andtranscriptional and translational stop signals.

The term “expression vector” as used herein, refers to a linear orcircular DNA molecule that comprises a polynucleotide encoding a variantand is operably linked to control sequences that provide for itsexpression.

The various nucleotide and control sequences may be joined together toproduce a recombinant expression vector that may include one or moreconvenient restriction sites to allow for insertion or substitution ofthe polynucleotide encoding the variant at such sites. Alternatively,the polynucleotide may be expressed by inserting the polynucleotide or anucleic acid construct comprising the polynucleotide into an appropriatevector for expression. In creating the expression vector, the codingsequence is located in the vector so that the coding sequence isoperably linked with the appropriate control sequences for expression.

The recombinant expression vector may be any vector (e.g., a plasmid orvirus) that can be conveniently subjected to recombinant DNA proceduresand can bring about expression of the polynucleotide. The choice of thevector will typically depend on the compatibility of the vector with thehost cell into which the vector is to be introduced. The vector may be alinear or closed circular plasmid.

The vector may be an autonomously replicating vector, i.e., a vectorthat exists as an extrachromosomal entity, the replication of which isindependent of chromosomal replication, e.g., a plasmid, anextrachromosomal element, a minichromosome, or an artificial chromosome.The vector may contain any means for assuring self-replication.Alternatively, the vector may be one that, when introduced into the hostcell, is integrated into the genome and replicated together with thechromosome(s) into which it has been integrated. Furthermore, a singlevector or plasmid or two or more vectors or plasmids that togethercontain the total DNA to be introduced into the genome of the host cell,or a transposon, may be used.

The vector preferably contains one or more selectable markers thatpermit easy selection of transformed, transfected, transduced, or thelike cells. A selectable marker is a gene the product of which providesfor biocide or viral resistance, resistance to heavy metals, prototrophyto auxotrophs, and the like.

The skilled person would know which expression vector is the mostsuitable for specific expression systems. Thus, the present invention isnot limited to any specific expression vector, but any expression vectorcomprising the polynucleotide encoding a variant according to theinvention is considered part of the present invention.

Host Cells

The present invention also relates to recombinant host cells, comprisinga polynucleotide encoding a variant of the present invention operablylinked to one or more control sequences that direct the production of avariant of the present invention. Thus, the present invention relates toa host cell, optionally a recombinant host cell, comprising apolynucleotide encoding a variant comprising a modification at one ormore positions corresponding to positions H1, H2, G7, I9, Q11, N16, N19,N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90,Q98, V104, G109, A111, F113, R116, Q118, Q125, R127, E130, S132, G133,T134, Y135, Q136, A139, G142, N144, G149, R158, Y160, D163, W167, Q169,S170, R171, Q172, L173, A174, N175, R176, Y178, R181, G182, A186, W187,N195, M202, Y203, A204, V206, D209, H210, E212, T227, L235, V238, M246,Q256, K259, V264, A265, E266, Y267, K269, N270, L272, G273, A274, L275,E276, W284, M286, V291, L293, Y295, Y298, Q299, N302, S303, S304, N306,R310, N311, N314, G315, L317, Q319, R320, S323, F328, G337, A339, Q345,D357, Q365, P377, D375, Q385, K391, Q395, R400, D406, W408, V410, S431,G435, W439, R444, Q445, R458, N465, Q466, W469, F473, G476 and V481,using SEQ ID NO: 1 for numbering operably linked to one or more controlsequences that direct the production of the variant.

The term “host cell” as used herein, refers to any cell type that issusceptible to transformation, transfection, transduction, or the likewith a nucleic acid construct or expression vector comprising apolynucleotide of the present invention. The term “host cell”encompasses any progeny of a parent cell that is not identical to theparent cell due to mutations that occur during replication.

A construct or vector comprising a polynucleotide is introduced into ahost cell so that the construct or vector is maintained as a chromosomalintegrant or as a self-replicating extrachromosomal vector as describedearlier. The choice of a host cell will to a large extent depend uponthe gene encoding the variant and its source.

The host cell may be any cell useful in the recombinant production of avariant, e.g., a prokaryote or a eukaryote.

The prokaryotic host cell may be any Gram-positive or Gram-negativebacterium. Gram-positive bacteria include, but are not limited to,Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus,Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus, andStreptomyces. Gram-negative bacteria include, but are not limited to,Campylobacter, E. coli, Flavobacterium, Fusobacterium, Helicobacter,Ilyobacter, Neisseria, Pseudomonas, Salmonella, and Ureaplasma.

Preparation of Variants

The present invention also relates to methods for obtaining a varianthaving alpha-amylase activity, comprising: (a) introducing into a parentalpha-amylase of SEQ ID NO: 2 a modification at one or more positionscorresponding to positions: H1, H2, G7, I9, Q11, N16, N19, N25, A37,W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90, Q98, V104,G109, A111, F113, R116, Q118, Q125, R127, E130, S132, G133, T134, Y135,Q136, A139, G142, N144, G149, R158, Y160, D163, W167, Q169, S170, R171,Q172, L173, A174, N175, R176, Y178, R181, G182, A186, W187, N195, M202,Y203, A204, V206, D209, H210, E212, T227, L235, V238, M246, Q256, K259,V264, A265, E266, Y267, K269, N270, L272, G273, A274, L275, E276, W284,M286, V291, L293, Y295, Y298, Q299, N302, S303, S304, N306, R310, N311,N314, G315, L317, Q319, R320, S323, F328, G337, A339, Q345, D357, Q365,P377, D375, Q385, K391, Q395, R400, D406, W408, V410, S431, G435, W439,R444, Q445, R458, N465, Q466, W469, F473, G476 and V481, using SEQ IDNO: 1 for numbering and the variant has alpha-amylase activity; and (b)recovering the variant.

The present invention also relates to methods for obtaining a varianthaving alpha-amylase activity, comprising: (a) introducing into a parentalpha-amylase of SEQ ID NO: 2 a modification at one or more positionscorresponding to position: H1*; H2*, G7A; I9M; Q11H; N16E; N16H; N16Y;N19D; N25K; N25M; N25T; A37V; W43Y; W48Y; N54Q; N54S; V56I; V56T; Y58F;G59A; A60S; A60T; L63F; T81A; E84Q; E86L; R90H; R90N; R90Q; Q98N; V104A;G109A; A111T; F113A; F113G; F113N; F113Q; F113T; F113Y; R116C; R116D;R116F; R116H; R116K; R116N; R116S; R116Y; Q118N; Q118T; Q125G; Q125S;R127A; R127F; R127H; R127L; R127N; R127T; E130D; E130F; E130I; E130K;E130L; E130N; E130S; E130T; E130V; S132A; S132D; S132F; S132L; S132M;S132P; S132T; G133D; T134C; T134E; T134N; T134P; T134R; T134S; T134W;T134Y; T134A; Y135H; Q136D; Q136E; Q136G; Q136N; Q136R; Q136S; A139T;G142H; N144H; G149A; R158C; R158H; R158K; R158Q; R158S; Y160D; Y160F;Y160H; Y160N; D163H; W167*; Q169*; Q169E; Q169F; S170*; 5170A; R171*;R171H; R171K; R171L; R171Y; Q172*; Q172K; Q172N; Q172S; L173*; L173K;A174*; A174N; A174S; N175*; N175S; R176*; R176A; Y178F; R181*; R181A;R181C; R181E; R181G; R181H; R181K; R181N; R181Q; G182*; G182T; A186D;A186E; A186H; A186K; A186N; A186T; W187M; N195D; N195F; N195H; N195Q;N195Y; M202L; Y203L; A204V; V206L; D209N; H210K; H210N; E212D; T227K;T227N; L235I; V238A; M246I; M246L; M246T; M246V; Q256H; Q256N; K259G;K259H; V264A; V246I; V264T; A265G; E266V; Y267F; Y267H; Y267L; K269M;K269Q; K269R; N270G; N270P; L272I; G273V; A274K; A274S; L275A; L275I;L275V; E276N; W284R; W284Y; M286L; V291A; V291I; V291T; L293I; L293V;Y295F; Y298E; Y298N; Q299N; Q299Y; N302A; N302H; N302K; N302Q; 5303G;5304G; 5304Q; N306H; N306K; N306Q; N306R; N306Y; R310N; R310S; R310Q;N311K; N311R; N314Q; G315N; L317V; Q319H; R320K; R320Q; R320S; S323T;H323K; F328L; G337D; G337E; A339S; Q345N; Q345R; L357F; Q365A; Q365C;Q365E; Q365H; Q365K; Q365M; Q365S; P377K; P377T; D375H; D375N; D375Y;Q385L; K391A; Q395K; R400S; D406H; W408H; V410I; S431F; G435C; W439R;W439T; R444T; Q445S; R458K; N465G; Q466S; W469N; W469Y; F473I; F473P;F473S; G476K; and V481, using SEQ ID NO: 1 for numbering.

The variants may be prepared using any mutagenesis procedure known inthe art, such as site-directed mutagenesis, synthetic gene construction,semi-synthetic gene construction, random mutagenesis, shuffling, etc.

Site-directed mutagenesis is a technique in which one or more (several)modifications are created at one or more defined sites in apolynucleotide encoding the parent.

Site-directed mutagenesis can be accomplished in vitro by PCR involvingthe use of oligonucleotide primers containing the desired modification.Site-directed mutagenesis can also be performed in vitro by cassettemutagenesis involving the cleavage by a restriction enzyme at a site inthe plasmid comprising a polynucleotide encoding the parent andsubsequent ligation of an oligonucleotide containing the modification inthe polynucleotide. Usually the restriction enzyme that digests at theplasmid and the oligonucleotide is the same, permitting sticky ends ofthe plasmid and insert to ligate to one another. See, e.g., Scherer andDavis, 1979, Proc. Natl. Acad. Sci. USA 76: 4949-4955; and Barton etal., 1990, Nucleic Acids Res. 18: 7349-4966.

Site-directed mutagenesis can also be accomplished in vivo by methodsknown in the art. See, e.g., U.S. Patent Application Publication No.2004/0171154; Storici et al., 2001, Nature Biotechnol. 19: 773-776; Krenet al., 1998, Nat. Med. 4: 285-290; and Calissano and Macino, 1996,Fungal Genet. Newslett. 43: 15-16.

Any site-directed mutagenesis procedure can be used in the presentinvention. There are many commercial kits available that can be used toprepare variants.

Synthetic gene construction entails in vitro synthesis of a designedpolynucleotide molecule to encode a polypeptide of interest. Genesynthesis can be performed utilizing a number of techniques, such as themultiplex microchip-based technology described by Tian et al. (2004,Nature 432: 1050-1054) and similar technologies wherein olgionucleotidesare synthesized and assembled upon photo-programable microfluidic chips.

Single or multiple amino acid substitutions, deletions, and/orinsertions can be made and tested using known methods of mutagenesis,recombination, and/or shuffling, followed by a relevant screeningprocedure, such as those disclosed by Reidhaar-Olson and Sauer, 1988,Science 241: 53-57; Bowie and Sauer, 1989, Proc. Natl. Acad. Sci. USA86: 2152-2156; WO 95/17413; or WO 95/22625. Other methods that can beused include error-prone PCR, phage display (e.g., Lowman et al., 1991,Biochemistry 30: 10832-10837; U.S. Pat. No. 5,223,409; WO 92/06204) andregion-directed mutagenesis (Derbyshire et al., 1986, Gene 46: 145; Neret al., 1988, DNA 7: 127).

Mutagenesis/shuffling methods can be combined with high-throughput,automated screening methods to detect activity of cloned, mutagenizedpolypeptides expressed by host cells (Ness et al., 1999, NatureBiotechnology 17: 893-896). Mutagenized DNA molecules that encode activepolypeptides can be recovered from the host cells and rapidly sequencedusing standard methods in the art. These methods allow the rapiddetermination of the importance of individual amino acid residues in apolypeptide.

Semi-synthetic gene construction is accomplished by combining aspects ofsynthetic gene construction, and/or site-directed mutagenesis, and/orrandom mutagenesis, and/or shuffling. Semi-synthetic construction istypified by a process utilizing polynucleotide fragments that aresynthesized, in combination with PCR techniques. Defined regions ofgenes may thus be synthesized de novo, while other regions may beamplified using site-specific mutagenic primers, while yet other regionsmay be subjected to error-prone PCR or non-error prone PCRamplification. Polynucleotide subsequences may then be shuffled.

Methods of Production

The present invention also relates to methods of producing analpha-amylase variant, comprising: (a) cultivating a host cell of thepresent invention under conditions suitable for the expression of thevariant; and (b) recovering the variant.

The host cells are cultivated in a nutrient medium suitable forproduction of the variant using methods known in the art. For example,the cell may be cultivated by shake flask cultivation, or small-scale orlarge-scale fermentation (including continuous, batch, fed-batch, orsolid state fermentations) in laboratory or industrial fermentorsperformed in a suitable medium and under conditions allowing thepolypeptide to be expressed and/or isolated. The cultivation takes placein a suitable nutrient medium comprising carbon and nitrogen sources andinorganic salts, using procedures known in the art. Suitable media areavailable from commercial suppliers or may be prepared according topublished compositions (e.g., in catalogues of the American Type CultureCollection). If the variant is secreted into the nutrient medium, thevariant can be recovered directly from the medium. If the variant is notsecreted, it can be recovered from cell lysates.

The variant may be detected using methods known in the art that arespecific for the variants. These detection methods may include use ofspecific antibodies, formation of an enzyme product, or disappearance ofan enzyme substrate. For example, an enzyme assay may be used todetermine the activity of the variant.

The variant may be recovered by methods known in the art. For example,the variant may be recovered from the nutrient medium by conventionalprocedures including, but not limited to, collection, centrifugation,filtration, extraction, spray-drying, evaporation, or precipitation.

The variant may be purified by a variety of procedures known in the artincluding, but not limited to, chromatography (e.g., ion exchange,affinity, hydrophobic, chromatofocusing, and size exclusion),electrophoretic procedures (e.g., preparative isoelectric focusing),differential solubility (e.g., ammonium sulfate precipitation),SDS-PAGE, or extraction (see, e.g., Protein Purification, J.-C. Jansonand Lars Ryden, editors, VCH Publishers, New York, 1989) to obtainsubstantially pure variants.

Alternatively, the variant is not recovered, but rather a host cell ofthe present invention expressing a variant is used as a source of thevariant.

Fermentation Broth Formulations or Cell Compositions

The present invention also relates to a fermentation broth formulationor a cell composition comprising a polypeptide of the present invention.Thus, in one embodiment, the fermentation broth formulation or the cellcomposition comprises a polynucleotide encoding a variant comprising amodification in one or more positions corresponding to the positions H1,H2, G7, I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60,L63, T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125,R127, E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158,Y160, D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178,R181, G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212,T227, L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270,L272, G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299,N302, S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323,F328, G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400,D406, W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469,F473, G476 and V481 and wherein the positions correspond to amino acidpositions in the amino acid sequence set forth in SEQ ID NO: 2, anucleic acid construct encoding a variant comprising an modification inone or more positions corresponding to the positions H1, H2, G7, I9,Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81,E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481 and wherein the positions correspond to amino acidpositions in the amino acid sequence set forth in SEQ ID NO: 2, or anexpression vector encoding a variant comprising an modification in oneor more positions corresponding to the positions H1, H2, G7, I9, Q11,N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84,E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127, E130,S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160, D163,W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181, G182,A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227, L235,V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272, G273,A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302, S303,S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328, G337,A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406, W408,V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473, G476,and V481 and wherein the positions correspond to amino acid positions inthe amino acid sequence set forth in SEQ ID NO: 2. The fermentationbroth product may further comprise additional ingredients used in thefermentation process, such as, for example, cells (including, the hostcells containing the gene encoding the polypeptide of the presentinvention which are used to produce the polypeptide of interest), celldebris, biomass, fermentation media and/or fermentation products. Insome embodiments, the composition is a cell-killed whole brothcontaining organic acid(s), killed cells and/or cell debris, and culturemedium.

The term “fermentation broth” as used herein refers to a preparationproduced by cellular fermentation that undergoes no or minimal recoveryand/or purification. For example, fermentation broths are produced whenmicrobial cultures are grown to saturation, incubated undercarbon-limiting conditions to allow protein synthesis (e.g., expressionof enzymes by host cells) and secretion into cell culture medium. Thefermentation broth can contain unfractionated or fractionated contentsof the fermentation materials derived at the end of the fermentation.Typically, the fermentation broth is unfractionated and comprises thespent culture medium and cell debris present after the microbial cells(e.g., filamentous fungal cells) are removed, e.g., by centrifugation.In some embodiments, the fermentation broth contains spent cell culturemedium, extracellular enzymes, and viable and/or nonviable microbialcells.

In one embodiment, the fermentation broth formulation and cellcompositions comprise a first organic acid component comprising at leastone 1-5 carbon organic acid and/or a salt thereof and a second organicacid component comprising at least one 6 or more carbon organic acidand/or a salt thereof. In a particular embodiment, the first organicacid component is acetic acid, formic acid, propionic acid, a saltthereof, or a mixture of two or more of the foregoing and the secondorganic acid component is benzoic acid, cyclohexanecarboxylic acid,4-methylvaleric acid, phenylacetic acid, a salt thereof, or a mixture oftwo or more of the foregoing.

In one embodiment, the composition contains an organic acid(s), andoptionally further contains killed cells and/or cell debris. In oneembodiment, the killed cells and/or cell debris are removed from acell-killed whole broth to provide a composition that is free of thesecomponents.

The fermentation broth formulations or cell compositions may furthercomprise a preservative and/or anti-microbial (e.g., bacteriostatic)agent, including, but not limited to, sorbitol, sodium chloride,potassium sorbate, and others known in the art.

The cell-killed whole broth or composition may comprise theunfractionated contents of the fermentation materials derived at the endof the fermentation. Typically, the cell-killed whole broth orcomposition comprises the spent culture medium and cell debris presentafter the microbial cells (e.g., filamentous fungal cells) are grown tosaturation, incubated under carbon-limiting conditions to allow proteinsynthesis. In some embodiments, the cell-killed whole broth orcomposition comprises the spent cell culture medium, extracellularenzymes, and killed filamentous fungal cells. In some embodiments, themicrobial cells present in the cell-killed whole broth or compositionmay be permeabilized and/or lysed using methods known in the art.

A whole broth or cell composition as described herein is typically aliquid, but may comprise insoluble components, such as killed cells,cell debris, culture media components, and/or insoluble enzyme(s). Insome embodiments, insoluble components may be removed to provide aclarified liquid composition.

The whole broth formulations and cell compositions of the presentinvention may be produced by a method described in WO 90/15861 or WO2010/096673.

Compositions

The present invention also relates to composition comprising a variantof the present invention. Accordingly, the present invention relates tocompositions comprising a variant having a modification at one or morepositions corresponding to positions: H1, H2, G7, I9, Q11, N16, N19,N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90,Q98, V104, G109, A111, F113, R116, Q118, Q125, R127, E130, S132, G133,T134, Y135, Q136, A139, G142, N144, G149, R158, Y160, D163, W167, Q169,S170, R171, Q172, L173, A174, N175, R176, Y178, R181, G182, A186, W187,N195, M202, Y203, A204, V206, D209, H210, E212, T227, L235, V238, M246,Q256, K259, V264, A265, E266, Y267, K269, N270, L272, G273, A274, L275,E276, W284, M286, V291, L293, Y295, Y298, Q299, N302, S303, S304, N306,R310, N311, N314, G315, L317, Q319, R320, S323, F328, G337, A339, Q345,D357, Q365, P377, D375, Q385, K391, Q395, R400, D406, W408, V410, S431,G435, W439, R444, Q445, R458, N465, Q466, W469, F473, G476 and V481 andwherein the positions correspond to amino acid positions in the aminoacid sequence set forth in SEQ ID NO: 2.

In one embodiment, the composition comprises a variant comprising

a) a deletion and/or a substitution at two or more positionscorresponding to positions R181, G182, D183, and G184 of the amino acidsequence as set forth in SEQ ID NO: 2, and

b) an modification at one or more positions corresponding to positionsH1, H2, G7, I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59,A60, L63, T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118,Q125, R127, E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149,R158, Y160, D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176,Y178, R181, G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210,E212, T227, L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269,N270, L272, G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298,Q299, N302, S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320,S323, F328, G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395,R400, D406, W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466,W469, F473, G476 and V481 of the amino acid sequence as set forth in SEQID NO: 2.

Preferably, the compositions are enriched in such a variant. The term“enriched” means that the alpha-amylase activity of the composition hasbeen increased, e.g., with an enrichment factor of 1.1.

In one embodiment, the invention is directed to compositions comprisinga variant of the present invention in combination with one or moreadditional components. The choice of additional components is within theskill of the artisan and includes conventional ingredients, includingthe exemplary non-limiting components set forth below.

In one embodiment, the present invention relates to a compositioncomprising one or more additional components selected from the groupconsisting of one or more enzymes, oxidizing agents, bleach activators,bleach catalysts, chelating agents, bulking agents, builders, bufferingagents, structurants, sequestrants, optical brighteners, antifoamingagents, enzymes, fragrances, anti-redeposition agents, skin conditioningagents, softness extenders, emulsifiers, crystal growth inhibitors,metal care agents, glass care agents and colorants.

In one embodiment, the present invention relates to a compositioncomprises a surfactant.

In one embodiment, the present invention relates to a compositionwherein the surfactant is one or more surfactants selected from thegroup consisting of an anionic surfactant, a cationic surfactant, anon-ionic surfactant, zwitterionic surfactant, and amphotericsurfactants or any mixtures thereof.

In one embodiment, the present invention relates to a compositionwherein the composition is a detergent composition.

In one embodiment, the composition is a liquid laundry or liquid dishwash composition, such as an Automatic Dish Wash (ADW) liquid detergentcomposition, or a powder laundry, such as a soap bar, or powder dishwash composition, such as an ADW unit dose detergent composition andsuch as a Hand Dish Wash (HDW) detergent composition.

In one embodiment, the present invention relates to a compositionwherein the composition comprises one or more additional enzymes.

The composition may comprise a variant as the major enzymatic component,e.g., a mono-component composition. Alternatively, the composition maycomprise multiple enzymatic activities, such as an proteases, amylases,phospho-lipases, esterases, lipases, cutinases, cellulases,endoglucanases, xyloglucanases, xylanases, pectinases, hemicellulasespectin lyases, xanthanases, peroxidases, keratinases haloperoxygenases,catalases, mannanases, lechinase, RNase, DNAse, reductases, oxidases,phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases,pento-sanases, malanases, β-glucanases, arabinosidases, hyaluronidase,chondroitinase, and laccase or any mixture thereof.

The additional enzyme(s) may be produced, for example, by amicroorganism belonging to the genus Bacillus, e.g. Bacilluslicheniformis and Bacillus subtilis, or the genus Aspergillus, e.g.,Aspergillus aculeatus, Aspergillus awamori, Aspergillus foetidus,Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans,Aspergillus niger, or Aspergillus oryzae; Fusarium, e.g., Fusariumbactridioides, Fusarium cerealis, Fusarium crookwellense, Fusariumculmorum, Fusarium graminearum, Fusarium graminum, Fusariumheterosporum, Fusarium negundi, Fusarium oxysporum, Fusariumreticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum,Fusarium sulphureum, Fusarium toruloseum, Fusarium trichothecioides, orFusarium venenatum; Humicola, e.g., Humicola insolens or Humicolalanuginosa; or Trichoderma, e.g., Trichoderma harzianum, Trichodermakoningii, Trichoderma longibrachiatum, Trichoderma reesei, orTrichoderma viride or any other host cell herein described.

The compositions may be prepared in accordance with methods known in theart and may be in the form of a liquid or a dry composition. Forinstance, the composition may be in the form of a granulate or amicrogranulate. The variant may be stabilized in accordance with methodsknown in the art.

Such compositions comprise a cleaning/detergent components, preferably amixture of components. Typically, the cleaning components will bepresent in the composition in an amount from 0.001 to 99.9 wt %, moretypically from 0.01 to 80 wt % cleaning component.

In another preferred aspect the composition comprises one or moresurfactants, which may be non-ionic including semi-polar and/or anionicand/or cationic and/or zwitterionic and/or ampholytic and/or semi-polarnonionic and/or mixtures thereof. The surfactants are typically presentat a level of from 0.1% to 60% by weight or from 0.5 to 50 wt % or 1 to40 wt % of the composition.

In one embodiment of the present invention, the variant of the presentinvention may be added to a detergent composition in an amountcorresponding to 0.001-100 mg of protein, such as 0.01-100 mg ofprotein, preferably 0.005-50 mg of protein, more preferably 0.01-25 mgof protein, even more preferably 0.05-10 mg of protein, most preferably0.05-5 mg of protein, and even most preferably 0.01-1 mg of protein perliter of wash liquor. The term “protein” in this context is contemplatedto be understood to include a variant according to the presentinvention.

A composition for use in automatic dish wash (ADW), for example, mayinclude 0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as0.05-5% of enzyme protein by weight of the composition.

A composition for use in laundry granulation, for example, may include0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as 0.05%-5% ofenzyme protein by weight of the composition.

A composition for use in laundry liquid, for example, may include0.0001%-10%, such as 0.001-7%, such as 0.1%-5% of enzyme protein byweight of the composition.

The variants of the invention as well as the further active components,such as additional enzymes, may be stabilized using conventionalstabilizing agents, e.g., a polyol such as propylene glycol or glycerol,a sugar or sugar alcohol, lactic acid, boric acid, or a boric acidderivative, e.g., an aromatic borate ester, or a phenyl boronic acidderivative such as 4-formylphenyl boronic acid, and the composition maybe formulated as described in, for example, WO92/19709 and WO92/19708.

In certain markets different wash conditions and, as such, differenttypes of detergents are used. This is disclosed in e.g. EP 1 025 240.For example, In Asia (Japan) a low detergent concentration system isused, while the United States uses a medium detergent concentrationsystem, and Europe uses a high detergent concentration system.

A low detergent concentration system includes detergents where less thanabout 800 ppm of detergent components are present in the wash water.Japanese detergents are typically considered low detergent concentrationsystem as they have approximately 667 ppm of detergent componentspresent in the wash water.

A medium detergent concentration includes detergents where between about800 ppm and about 2000 ppm of detergent components are present in thewash water. North American detergents are generally considered to bemedium detergent concentration systems as they have approximately 975ppm of detergent components present in the wash water.

A high detergent concentration system includes detergents where greaterthan about 2000 ppm of detergent components are present in the washwater. European detergents are generally considered to be high detergentconcentration systems as they have approximately 4500-5000 ppm ofdetergent components in the wash water.

Latin American detergents are generally high suds phosphate builderdetergents and the range of detergents used in Latin America can fall inboth the medium and high detergent concentrations as they range from1500 ppm to 6000 ppm of detergent components in the wash water. Suchdetergent compositions are all embodiments of the invention.

A variant of the present invention may also be incorporated in thedetergent formulations disclosed in WO97/07202, which is herebyincorporated by reference.

Examples are given herein of preferred uses of the compositions of thepresent invention. The dosage of the composition and other conditionsunder which the composition is used may be determined on the basis ofmethods known in the art.

In particular, a composition according to the present invention furthercomprises a chelator.

The term “chelator” as used herein, refers to chemicals which formmolecules with certain metal ions, inactivating the ions so that theycannot react with other elements. Thus, a chelator may be defined as abinding agent that suppresses chemical activity by forming chelates.Chelation is the formation or presence of two or more separate bindingsbetween a ligand and a single central atom. The ligand may be anyorganic compound, a silicate or a phosphate. In the present context theterm “chelating agents” comprises chelants, chelating agent, chelatingagents, complexing agents, or sequestering agents that formswater-soluble complexes with metal ions such as calcium and magnesium.The chelate effect describes the enhanced affinity of chelating ligandsfor a metal ion compared to the affinity of a collection of similarnonchelating ligands for the same metal. Chelating agents having bindingcapacity with metal ions, in particular calcium (Ca2+) ions, and hasbeen used widely in detergents and compositions in general for wash,such as laundry or dish wash. Chelating agents have however shownthemselves to inhibit enzymatic activity. The term chelating agent isused in the present application interchangeably with “complexing agent”or “chelating agent” or “chelant”.

Since most alpha-amylases are calcium sensitive the presence ofchelating agents these may impair the enzyme activity. The calciumsensitivity of alpha-amylases can be determined by incubating a givenalpha-amylase in the presence of a strong chelating agent and analyzethe impact of this incubation on the activity of the alpha-amylase inquestion. A calcium sensitive alpha-amylase will lose a major part orall of its activity during the incubation. Chelating agent may bepresent in the composition in an amount from 0.0001 wt % to 20 wt %,preferably from 0.01 to 10 wt %, more preferably from 0.1 to 5 wt %.

Non-limiting examples of chelating agents are; EDTA, DTMPA, HEDP, andcitrate. Thus, in one embodiment, the composition comprises a variantaccording to the invention and a chelating agent, such as EDTA, DTMPA,HEDP or citrate.

The term “EDTA” as used herein, refers to ethylene-diamine-tetra-aceticacid which falls under the definition of “strong chelating agents”.

The term “DTMPA” as used herein, refers to diethylenetriaminepenta(methylene phosphonic acid). DTMPA can inhibit the scale formationof carbonate, sulfate and phosphate.

The term “HEDP” as used herein, refers to hydroxy-ethane diphosphonicacid, which falls under the definition of “strong chelating agents”.

The chelate effect or the chelating effect describes the enhancedaffinity of chelating ligands for a metal ion compared to the affinityof a collection of similar nonchelating ligands for the same metal.However, the strength of this chelate effect can be determined byvarious types of assays or measure methods thereby differentiating orranking the chelating agents according to their chelating effect (orstrength).

In an assay the chelating agents may be characterized by their abilityto reduce the concentration of free calcium ions (Ca2+) from 2.0 mM to0.10 mM or less at pH 8.0, e.g. by using a test based on the methoddescribed by M. K. Nagarajan et al., JAOCS, Vol. 61, no. 9 (September1984), pp. 1475-1478.

For reference, a chelator having the same ability to reduce theconcentration of free calcium ions (Ca2+) from 2.0 mM to 0.10 mM at pHas EDTA at equal concentrations of the chelator are said to be strongchelators.

The composition of the present invention may be in any convenient form,e.g., a bar, a homogenous tablet, a tablet having two or more layers, apouch having one or more compartments, a regular or compact powder, agranule, a paste, a gel, or a regular, compact or concentrated liquid.There are a number of detergent formulation forms such as layers (sameor different phases), pouches, as well as forms for machine dosing unit.

Pouches can be configured as single or multicompartments. It can be ofany form, shape and material which is suitable for hold the composition,e.g. without allowing the release of the composition from the pouchprior to water contact. The pouch is made from water soluble film whichencloses an inner volume. Said inner volume can be divided intocompartments of the pouch. Preferred films are polymeric materialspreferably polymers which are formed into a film or sheet. Preferredpolymers, copolymers or derivatives thereof are selected polyacrylates,and water soluble acrylate copolymers, methyl cellulose, carboxy methylcellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose,hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, mostpreferably polyvinyl alcohol copolymers and, hydroxypropyl methylcellulose (HPMC). Preferably the level of polymer in the film forexample PVA is at least about 60%. Preferred average molecular weightwill typically be about 20,000 to about 150,000. Films can also be ofblend compositions comprising hydrolytically degradable and watersoluble polymer blends such as polyactide and polyvinyl alcohol (knownunder the Trade reference M8630 as sold by Chris Craft In. Prod. OfGary, Ind., US) plus plasticisers like glycerol, ethylene glycerol,Propylene glycol, sorbitol and mixtures thereof. The pouches cancomprise a solid laundry cleaning composition or part components and/ora liquid cleaning composition or part components separated by the watersoluble film. The compartment for liquid components can be different incomposition than compartments containing solids. Ref: (US2009/0011970A1).

Detergent ingredients may be separated physically from each other bycompartments in water dissolvable pouches or in different layers oftablets. Thereby negative storage interaction between components may beavoided. Different dissolution profiles of each of the compartments canalso give rise to delayed dissolution of selected components in the washsolution.

A liquid or gel detergent, which is not unit dosed, may be aqueous,typically containing at least 20% by weight and up to 95% water, such asup to about 70% water, up to about 65% water, up to about 55% water, upto about 45% water, up to about 35% water. Other types of liquids,including without limitation, alkanols, amines, diols, ethers andpolyols may be included in an aqueous liquid or gel. An aqueous liquidor gel detergent may contain from 0-30% organic solvent. A liquid or geldetergent may be non-aqueous.

Another form of composition is in the form of a soap bar, such as alaundry soap bar, and may be used for hand washing laundry, fabricsand/or textiles. The term “soap bar” as used herein, refers to includeslaundry bars, soap bars, combo bars, syndet bars and detergent bars. Thetypes of bar usually differ in the type of surfactant they contain, andthe term laundry soap bar includes those containing soaps from fattyacids and/or synthetic soaps. The laundry soap bar has a physical formwhich is solid and not a liquid, gel or a powder at room temperature.The term “solid” as used herein, refers to a physical form which doesnot significantly change over time, i.e. if a solid object (e.g. laundrysoap bar) is placed inside a container, the solid object does not changeto fill the container it is placed in. The bar is a solid typically inbar form but can be in other solid shapes such as round or oval.

The soap bar may also comprise complexing agents like EDTA and HEDP,perfumes and/or different type of fillers, surfactants e.g. anionicsynthetic surfactants, builders, polymeric soil release agents,detergent chelators, stabilizing agents, fillers, dyes, colorants, dyetransfer inhibitors, alkoxylated polycarbonates, suds suppressers,structurants, binders, leaching agents, bleaching activators, clay soilremoval agents, anti-redeposition agents, polymeric dispersing agents,brighteners, fabric softeners, perfumes and/or other compounds known inthe art.

The soap bar may be processed in conventional laundry soap bar makingequipment such as but not limited to: mixers, plodders, e.g. a two stagevacuum plodder, extruders, cutters, logo-stampers, cooling tunnels andwrappers. The invention is not limited to preparing the soap bars by anysingle method. The premix of the invention may be added to the soap atdifferent stages of the process. For example, the premix comprising asoap, an enzyme, optionally one or more additional enzymes, a proteaseinhibitor, and a salt of a monovalent cation and an organic anion may beprepared and the mixture may then plodded. The enzyme and optionaladditional enzymes may be added at the same time as an enzyme inhibitor,e.g. a protease inhibitor, for example in liquid form. Besides themixing step and the plodding step, the process may further comprise thesteps of milling, extruding, cutting, stamping, cooling and/or wrapping.

Surfactants

The detergent composition may comprise one or more surfactants, whichmay be anionic and/or cationic and/or non-ionic and/or semi-polar and/orzwitterionic, or a mixture thereof. In a particular embodiment, thedetergent composition includes a mixture of one or more nonionicsurfactants and one or more anionic surfactants. The surfactant(s) istypically present at a level of from about 0.1% to 60% by weight, suchas about 1% to about 40%, or about 3% to about 20%, or about 3% to about10%. The surfactant(s) is chosen based on the desired cleaningapplication, and includes any conventional surfactant(s) known in theart. Any surfactant known in the art for use in detergents may beutilized.

When included therein the detergent will usually contain from about 1%to about 40% by weight, such as from about 5% to about 30%, includingfrom about 5% to about 15%, or from about 20% to about 25% of an anionicsurfactant. Non-limiting examples of anionic surfactants includesulfates and sulfonates, in particular, linear alkylbenzenesulfonates(LAS), isomers of LAS, branched alkylbenzenesulfonates (BABS),phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates,alkene sulfonates, alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonatesand disulfonates, alkyl sulfates (AS) such as sodium dodecyl sulfate(SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS),alcohol ethersulfates (AES or AEOS or FES, also known as alcoholethoxysulfates or fatty alcohol ether sulfates), secondaryalkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates,sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methylesters (alpha-SFMe or SES) including methyl ester sulfonate (MES),alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid(DTSA), fatty acid derivatives of amino acids, diesters and monoestersof sulfo-succinic acid or soap, and combinations thereof.

When included therein the detergent will usually contain from about 0%to about 40% by weight of a cationic surfactant. Non-limiting examplesof cationic surfactants include alklydimethylethanolamine quat (ADMEAQ),cetyltrimethylammonium bromide (CTAB), dimethyldistearylammoniumchloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternaryammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, andcombinations thereof.

When included therein the detergent will usually contain from about 0.2%to about 40% by weight of a non-ionic surfactant, for example from about0.5% to about 30%, in particular from about 1% to about 20%, from about3% to about 10%, such as from about 3% to about 5%, or from about 8% toabout 12%. Non-limiting examples of non-ionic surfactants includealcohol ethoxylates (AE or AEO), alcohol propoxylates, propoxylatedfatty alcohols (PFA), epoxy-capped poly(oxyalkylated) alcohols,alkoxylated fatty acid alkyl esters, such as ethoxylated and/orpropoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE),nonylphenol ethoxylates (NPE), alkylpolyglycosides (APG), alkoxylatedamines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides(FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylatedfatty acid monoethanolamides (PFAM), polyhydroxy alkyl fatty acidamides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, orfatty acid glucamide, FAGA), as well as products available under thetrade names SPAN and TWEEN, and combinations thereof.

When included therein the detergent will usually contain from about 0%to about 40% by weight of a semipolar surfactant. Non-limiting examplesof semipolar surfactants include amine oxides (AO) such asalkyldimethylamineoxide, N-(coco alkyl)-N,N-dimethylamine oxide andN-(tallow-alkyl)-N,N-bis(2-hydroxyethyl)amine oxide, fatty acidalkanolamides and ethoxylated fatty acid alkanolamides, and combinationsthereof.

When included therein the detergent will usually contain from about 0%to about 40% by weight of a zwitterionic surfactant. Non-limitingexamples of zwitterionic surfactants include betaine,alkyldimethylbetaine, sulfobetaine, and combinations thereof.

The detergent composition may also comprise one or more isoprenoidsurfactants as disclosed in US 20130072416 or US 20130072415.

Hydrotropes

A hydrotrope is a compound that solubilises hydrophobic compounds inaqueous solutions (or oppositely, polar substances in a non-polarenvironment). Typically, hydrotropes have both hydrophilic and ahydrophobic character (so-called amphiphilic properties as known fromsurfactants); however the molecular structure of hydrotropes generallydo not favor spontaneous self-aggregation, see e.g. review by Hodgdonand Kaler (2007), Current Opinion in Colloid & Interface Science 12:121-128. Hydrotropes do not display a critical concentration above whichself-aggregation occurs as found for surfactants and lipids formingmiceller, lamellar or other well defined meso-phases. Instead, manyhydrotropes show a continuous-type aggregation process where the sizesof aggregates grow as concentration increases. However, many hydrotropesalter the phase behavior, stability, and colloidal properties of systemscontaining substances of polar and non-polar character, includingmixtures of water, oil, surfactants, and polymers. Hydrotropes areclassically used across industries from pharma, personal care, food, totechnical applications. Use of hydrotropes in detergent compositionsallow for example more concentrated formulations of surfactants (as inthe process of compacting liquid detergents by removing water) withoutinducing undesired phenomena such as phase separation or high viscosity.

The detergent may contain 0-5% by weight, such as about 0.5 to about 5%,or about 3% to about 5%, of a hydrotrope. Any hydrotrope known in theart for use in detergents may be utilized. Non-limiting examples ofhydrotropes include sodium benzene sulfonate, sodium p-toluene sulfonate(STS), sodium xylene sulfonate (SXS), sodium cumene sulfonate (SCS),sodium cymene sulfonate, amine oxides, alcohols and polyglycolethers,sodium hydroxynaphthoate, sodium hydroxynaphthalene sulfonate, sodiumethylhexyl sulfate, and combinations thereof.

Builders and Co-Builders

The detergent composition may contain about 0-65% by weight, such asabout 5% to about 50% of a detergent builder or co-builder, or a mixturethereof. In a dish wash detergent, the level of builder is typically40-65%, particularly 50-65%. The builder and/or co-builder mayparticularly be a chelating agent that forms water-soluble complexeswith Ca and Mg. Any builder and/or co-builder known in the art for usein laundry/ADW/hard surface cleaning detergents may be utilized.Non-limiting examples of builders include zeolites, diphosphates(pyrophosphates), triphosphates such as sodium triphosphate (STP orSTPP), carbonates such as sodium carbonate, soluble silicates such assodium metasilicate, layered silicates (e.g., SKS-6 from Hoechst),ethanolamines such as 2-aminoethan-1-ol (MEA), diethanolamine (DEA, alsoknown as 2,2′-iminodiethan-1-01), triethanolamine (TEA, also known as2,2′,2″-nitrilotriethan-1-ol), and (carboxymethyl)inulin (CMI), andcombinations thereof.

The detergent composition may also contain 0-50% by weight, such asabout 5% to about 30%, of a detergent co-builder. The detergentcomposition may include a co-builder alone, or in combination with abuilder, for example a zeolite builder. Non-limiting examples ofco-builders include homopolymers of polyacrylates or copolymers thereof,such as poly(acrylic acid) (PAA) or copoly(acrylic acid/maleic acid)(PAA/PMA). Further non-limiting examples include citrate, chelators suchas aminocarboxylates, aminopolycarboxylates and phosphonates, and alkyl-or alkenylsuccinic acid. Additional specific examples include2,2′,2″-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid(EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid(IDS), ethylenediamine-N, N′-disuccinic acid (EDDS),methylglycinediacetic acid (MGDA), glutamic acid-N,N-diacetic acid(GLDA), 1-hydroxyethane-1,1-diphosphonic acid (H EDP),ethylenediaminetetra(methylenephosphonic acid) (EDTMPA),diethylenetriaminepentakis(methylenephosphonic acid) (DTMPA or DTPMPA),N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoaceticacid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), asparticacid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA),N-(2-sulfomethyl)-aspartic acid (SMAS), N-(2-sulfoethyl)-aspartic acid(SEAS), N-(2-sulfomethyl)-glutamic acid (SMGL),N-(2-sulfoethyl)-glutamic acid (SEGL), N-methyliminodiacetic acid(MIDA), α-alanine-N,N-diacetic acid (α-ALDA), serine-N,N-diacetic acid(SEDA), isoserine-N,N-diacetic acid (ISDA), phenylalanine-N,N-diaceticacid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilicacid-N,N-diacetic acid (SLDA), taurine-N,N-diacetic acid (TUDA) andsulfomethyl-N,N-diacetic acid (SMDA),N-(2-hydroxyethyl)ethylenediamine-N,N′,N″-triacetic acid (HEDTA),diethanolglycine (DEG), diethylenetriamine penta(methylenephosphonicacid) (DTPMP), aminotris(methylenephosphonic acid) (ATMP), andcombinations and salts thereof. Further exemplary builders and/orco-builders are described in, e.g., WO 09/102854, U.S. Pat. No.5,977,053

Bleaching Systems

The detergent may contain 0-30% by weight, such as about 1% to about20%, of a bleaching system. Any bleaching system known in the art foruse in laundry/ADW/hard surface cleaning detergents may be utilized.Suitable bleaching system components include bleaching catalysts,photobleaches, bleach activators, sources of hydrogen peroxide such assodium percarbonate, sodium perborates and hydrogen peroxide—urea (1:1),preformed peracids and mixtures thereof. Suitable preformed peracidsinclude, but are not limited to, peroxycarboxylic acids and salts,diperoxydicarboxylic acids, perimidic acids and salts,peroxymonosulfuric acids and salts, for example, Oxone (R), and mixturesthereof. Non-limiting examples of bleaching systems includeperoxide-based bleaching systems, which may comprise, for example, aninorganic salt, including alkali metal salts such as sodium salts ofperborate (usually mono- or tetra-hydrate), percarbonate, persulfate,perphosphate, persilicate salts, in combination with a peracid-formingbleach activator. The term bleach activator is meant herein as acompound which reacts with hydrogen peroxide to form a peracid viaperhydrolysis. The peracid thus formed constitutes the activated bleach.Suitable bleach activators to be used herein include those belonging tothe class of esters, amides, imides or anhydrides. Suitable examples aretetraacetylethylenediamine (TAED), acylated triazine derivatives, inparticular 1,5-diacetyl-2,4-dioxohexahydro-1,3,5-triazine (DADHT),acylated glycolurils, in particular tetraacetylglycoluril (TAGU).N-acylimides, in particular N-nonanoylsuccinimide (NOSI), sodium4-[(3,5,5-trimethylhexanoy)oxy]benzene-1-sulfonate (ISONOBS),4-(dodecanoyloxy)benzene-1-sulfonate (LOBS),4-(decanoyloxy)benzene-1-sulfonate, 4-(decanoyloxy)benzoate (DOBS orDOBA), 4-(nonanoyloxy)benzene-1-sulfonate (NOBS), and/or those disclosedin WO98/17767. A particular family of bleach activators of interest wasdisclosed in EP624154 and particularly preferred in that family isacetyl triethyl citrate (ATC). ATC or a short chain triglyceride liketriacetin has the advantage that it is environmentally friendlyFurthermore acetyl triethyl citrate and triacetin have good hydrolyticalstability in the product upon storage and are efficient bleachactivators. Finally ATC is multifunctional, as the citrate released inthe perhydrolysis reaction may function as a builder. Alternatively, thebleaching system may comprise peroxyacids of, for example, the amide,imide, or sulfone type. The bleaching system may also comprise peracidssuch as 6-(phthalimido)peroxyhexanoic acid (PAP). The bleaching systemmay also include a bleach catalyst, for example manganesetriazacyclononane, manganese oxalate, Co, Cu, Mn and Fe bispyridylamineand pentamine acetate cobalt(III). In some embodiments the bleachcomponent may be an organic catalyst selected from the group consistingof organic catalysts having the following formulae:

(iii) and mixtures thereof;

wherein each R¹ is independently a branched alkyl group containing from9 to 24 carbons or linear alkyl group containing from 11 to 24 carbons,preferably each R¹ is independently a branched alkyl group containingfrom 9 to 18 carbons or linear alkyl group containing from 11 to 18carbons, more preferably each R¹ is independently selected from thegroup consisting of 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl,2-hexyldecyl, dodecyl, tetradecyl, hexadecyl, octadecyl, isononyl,isodecyl, isotridecyl and isopentadecyl. Other exemplary bleachingsystems are described, e.g. in WO2007/087258, WO2007/087244,WO2007/087259, EP1867708 (Vitamin K) and WO2007/087242. Suitablephotobleaches may for example be sulfonated zinc or aluminiumphthalocyanines.

Preferably the bleach component comprises a source of peracid inaddition to bleach catalyst, particularly organic bleach catalyst. Thesource of peracid may be selected from (a) preformed peracid; (b)percarbonate, perborate or persulfate salt (hydrogen peroxide source)preferably in combination with a bleach activator; and (c) perhydrolaseenzyme and an ester for forming peracid in situ in the presence of waterin a textile or hard surface treatment step.

Polymers

The detergent may contain 0-10% by weight, such as 0.5-5%, 2-5%, 0.5-2%or 0.2-1% of a polymer. Any polymer known in the art for use indetergents may be utilized. The polymer may function as a co-builder asmentioned above, or may provide antiredeposition, fiber protection, soilrelease, dye transfer inhibition, grease cleaning and/or anti-foamingproperties. Some polymers may have more than one of the above-mentionedproperties and/or more than one of the below-mentioned motifs. Exemplarypolymers include (carboxymethyl)cellulose (CMC), poly(vinyl alcohol)(PVA), poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) orpoly(ethylene oxide) (PEG), ethoxylated poly(ethyleneimine),carboxymethyl inulin (CMI), and polycarboxylates such as PAA, PAA/PMA,poly-aspartic acid, and lauryl methacrylate/acrylic acid copolymers,hydrophobically modified CMC (HM-CMC) and silicones, copolymers ofterephthalic acid and oligomeric glycols, copolymers of poly(ethyleneterephthalate) and poly(oxyethene terephthalate) (PET-POET), PVP,poly(vinylimidazole) (PVI), poly(vinylpyridine-N-oxide) (PVPO or PVPNO)and polyvinylpyrrolidone-vinylimidazole (PVPVI). Further exemplarypolymers include sulfonated polycarboxylates, polyethylene oxide andpolypropylene oxide (PEO-PPO) and diquaternium ethoxy sulfate. Otherexemplary polymers are disclosed in, e.g., WO 2006/130575. Salts of theabove-mentioned polymers are also contemplated.

Fabric Hueing Agents

The detergent compositions of the present invention may also includefabric hueing agents such as dyes or pigments, which when formulated indetergent compositions can deposit onto a fabric when said fabric iscontacted with a wash liquor comprising said detergent compositions andthus altering the tint of said fabric through absorption/reflection ofvisible light. Fluorescent whitening agents emit at least some visiblelight. In contrast, fabric hueing agents alter the tint of a surface asthey absorb at least a portion of the visible light spectrum. Suitablefabric hueing agents include dyes and dye-clay conjugates, and may alsoinclude pigments. Suitable dyes include small molecule dyes andpolymeric dyes. Suitable small molecule dyes include small molecule dyesselected from the group consisting of dyes falling into the Colour Index(C.I.) classifications of Direct Blue, Direct Red, Direct Violet, AcidBlue, Acid Red, Acid Violet, Basic Blue, Basic Violet and Basic Red, ormixtures thereof, for example as described in WO2005/03274,WO2005/03275, WO2005/03276 and EP1876226 (hereby incorporated byreference). The detergent composition preferably comprises from about0.00003 wt % to about 0.2 wt %, from about 0.00008 wt % to about 0.05 wt%, or even from about 0.0001 wt % to about 0.04 wt % fabric hueingagent. The composition may comprise from 0.0001 wt % to 0.2 wt % fabrichueing agent, this may be especially preferred when the composition isin the form of a unit dose pouch. Suitable hueing agents are alsodisclosed in, e.g. WO 2007/087257 and WO2007/087243.

Adjunct Materials

Any detergent components known in the art for use in laundry, ADW orhard surface cleaning detergents may also be utilized. Other optionaldetergent components include anti-corrosion agents, anti-shrink agents,anti-soil redeposition agents, anti-wrinkling agents, bactericides,binders, corrosion inhibitors, metal care agents, glass care agents,disintegrants/disintegration agents, dyes, enzyme stabilizers (includingboric acid, borates, CMC, and/or polyols such as propylene glycol),fabric conditioners including clays, fillers/processing aids,fluorescent whitening agents/optical brighteners, foam boosters, foam(suds) regulators, perfumes, soil-suspending agents, softeners, sudssuppressors, tarnish inhibitors, and wicking agents, either alone or incombination. Any ingredient known in the art for use in laundry, ADW orhard surface cleaning detergents may be utilized. The choice of suchingredients is well within the skill of the artisan.

Dispersants

The detergent compositions of the present invention can also containdispersants. In particular powdered detergents may comprise dispersants.Suitable water-soluble organic materials include the homo- orco-polymeric acids or their salts, in which the polycarboxylic acidcomprises at least two carboxyl radicals separated from each other bynot more than two carbon atoms. Suitable dispersants are for exampledescribed in Powdered Detergents, Surfactant science series volume 71,Marcel Dekker, Inc.

Dye Transfer Inhibiting Agents

The detergent compositions of the present invention may also include oneor more dye transfer inhibiting agents. Suitable polymeric dye transferinhibiting agents include, but are not limited to, polyvinylpyrrolidonepolymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidoneand N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles ormixtures thereof. When present in a subject composition, the dyetransfer inhibiting agents may be present at levels from about 0.0001%to about 10%, from about 0.01% to about 5% or even from about 0.1% toabout 3% by weight of the composition.

Fluorescent Whitening Agent

The detergent compositions of the present invention will preferably alsocontain additional components that may tint articles being cleaned, suchas fluorescent whitening agent or optical brighteners. Where present thebrightener is preferably at a level of about 0.01% to about 0.5%. Anyfluorescent whitening agent suitable for use in a laundry detergentcomposition may be used in the composition of the present invention. Themost commonly used fluorescent whitening agents are those belonging tothe classes of diaminostilbene-sulfonic acid derivatives,diarylpyrazoline derivatives and bisphenyl-distyryl derivatives.Examples of the diaminostilbene-sulfonic acid derivative type offluorescent whitening agents include the sodium salts of:4,4′-bis-(2-diethanolamino-4-anilino-s-triazin-6-ylamino)stilbene-2,2′-disulfonate, 4,4′-bis-(2,4-dianilino-s-triazin-6-ylamino)stilbene-2.2′-disulfonate,4,4′-bis-(2-anilino-4-(N-methyl-N-2-hydroxy-ethylamino)-s-triazin-6-ylamino)stilbene-2,2′-disulfonate,4,4′-bis-(4-phenyl-1,2,3-triazol-2-yl)stilbene-2,2′-disulfonate andsodium5-(2H-naphtho[1,2-d][1,2,3]triazol-2-yl)-2-[(E)-2-phenylvinyl]benzenesulfonate.Preferred fluorescent whitening agents are Tinopal DMS and Tinopal CBSavailable from Ciba-Geigy AG, Basel, Switzerland. Tinopal DMS is thedisodium salt of 4,4′-bis-(2-morpholino-4-anilino-s-triazin-6-ylamino)stilbene-2,2′-disulfonate. Tinopal CBS is the disodium salt of2,2′-bis-(phenyl-styryl)-disulfonate. Also preferred are fluorescentwhitening agents is the commercially available Parawhite KX, supplied byParamount Minerals and Chemicals, Mumbai, India. Other fluorescerssuitable for use in the invention include the 1-3-diaryl pyrazolines andthe 7-alkylaminocoumarins.

Suitable fluorescent brightener levels include lower levels of fromabout 0.01, from 0.05, from about 0.1 or even from about 0.2 wt % toupper levels of 0.5 or even 0.75 wt %.

Soil Release Polymers

The detergent compositions of the present invention may also include oneor more soil release polymers which aid the removal of soils fromfabrics such as cotton and polyester based fabrics, in particular theremoval of hydrophobic soils from polyester based fabrics. The soilrelease polymers may for example be nonionic or anionic terephthaltebased polymers, polyvinyl caprolactam and related copolymers, vinylgraft copolymers, polyester polyamides see for example Chapter 7 inPowdered Detergents, Surfactant science series volume 71, Marcel Dekker,Inc. Another type of soil release polymers are amphiphilic alkoxylatedgrease cleaning polymers comprising a core structure and a plurality ofalkoxylate groups attached to that core structure. The core structuremay comprise a polyalkylenimine structure or a polyalkanolaminestructure as described in detail in WO 2009/087523 (hereby incorporatedby reference). Furthermore random graft co-polymers are suitable soilrelease polymers. Suitable graft co-polymers are described in moredetail in WO 2007/138054, WO 2006/108856 and WO 2006/113314 (herebyincorporated by reference). Other soil release polymers are substitutedpolysaccharide structures especially substituted cellulosic structuressuch as modified cellulose deriviatives such as those described in EP1867808 or WO 2003/040279 (both are hereby incorporated by reference).Suitable cellulosic polymers include cellulose, cellulose ethers,cellulose esters, cellulose amides and mixtures thereof. Suitablecellulosic polymers include anionically modified cellulose, nonionicallymodified cellulose, cationically modified cellulose, zwitterionicallymodified cellulose, and mixtures thereof. Suitable cellulosic polymersinclude methyl cellulose, carboxy methyl cellulose, ethyl cellulose,hydroxyl ethyl cellulose, hydroxyl propyl methyl cellulose, estercarboxy methyl cellulose, and mixtures thereof.

Anti-Redeposition Agents

The detergent compositions of the present invention may also include oneor more anti-redeposition agents such as carboxymethylcellulose (CMC),polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), polyoxyethyleneand/or polyethyleneglycol (PEG), homopolymers of acrylic acid,copolymers of acrylic acid and maleic acid, and ethoxylatedpolyethyleneimines. The cellulose based polymers described under soilrelease polymers above may also function as anti-redeposition agents.

Rheology Modifiers

The detergent compositions of the present invention may also include oneor more rheology modifiers, structurants or thickeners, as distinct fromviscosity reducing agents. The rheology modifiers are selected from thegroup consisting of non-polymeric crystalline, hydroxy-functionalmaterials, polymeric rheology modifiers which impart shear thinningcharacteristics to the aqueous liquid matrix of a liquid detergentcomposition. The rheology and viscosity of the detergent can be modifiedand adjusted by methods known in the art, for example as shown in EP2169040.

Other suitable adjunct materials include, but are not limited to,anti-shrink agents, anti-wrinkling agents, bactericides, binders,carriers, dyes, enzyme stabilizers, fabric softeners, fillers, foamregulators, hydrotropes, perfumes, pigments, sod suppressors, solvents,and structurants for liquid detergents and/or structure elasticizingagents.

Enzymes

In one embodiment, the composition according to the invention comprisesone or more further enzymes, such as at least two enzymes, morepreferred at least three, four or five enzymes. Preferably, the enzymesof the detergent composition have different substrate specificity, e.g.,proteolytic activity, amylolytic activity, lipolytic activity,cellulytic activity, hemicellulytic activity, oxidative activity, RNAseactivity, DNAse activity or pectolytic activity.

The composition according to the invention may comprise one or moreadditional enzymes selected from proteases, second amylases, lipases,cutinases, cellulases, endoglucanases, lechinase, xyloglucanases,pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases,catalases, mannanases, or any mixture thereof. Other suitable enzymesinclude carbohydrate-active enzymes like carbohydrase, arabinase,galactanase, xylanase; or oxidases, e.g., a laccase, and/or peroxidase.

In general the properties of the selected enzyme(s) should be compatiblewith the selected detergent, (i.e., pH-optimum, compatibility with otherenzymatic and non-enzymatic ingredients, etc.), and the enzyme(s) shouldbe present in effective amounts.

Cellulases

Suitable cellulases include those of bacterial or fungal origin.Chemically modified or protein engineered mutants are included. Suitablecellulases include cellulases from the genera Bacillus, Pseudomonas,Humicola, Fusarium, Thielavia, Acremonium, e.g., the fungal cellulasesproduced from Humicola insolens, Myceliophthora thermophila and Fusariumoxysporum disclosed in U.S. Pat. Nos. 4,435,307, 5,648,263, 5,691,178,5,776,757 and WO 89/09259.

Especially suitable cellulases are the alkaline or neutral cellulaseshaving colour care benefits. Examples of such cellulases are cellulasesdescribed in EP 0 495 257, EP 0 531 372, WO 96/11262, WO 96/29397, WO98/08940. Other examples are cellulase variants such as those describedin WO 94/07998, EP 0 531 315, U.S. Pat. Nos. 5,457,046, 5,686,593,5,763,254, WO 95/2447I, WO 98/12307 and WO99/001544.

Other cellulases are endo-beta-1,4-glucanase enzyme having a sequence ofat least 97% identity to the amino acid sequence of position 1 toposition 773 of SEQ ID NO:2 of WO 2002/099091 or a family 44xyloglucanase, which a xyloglucanase enzyme having a sequence of atleast 60% identity to positions 40-559 of SEQ ID NO: 2 of WO2001/062903.

Commercially available cellulases include Celluzyme™, and Carezyme™(Novozymes A/S) Carezyme Premium™ (Novozymes A/S), Celluclean™(Novozymes A/S), Celluclean Classic™ (Novozymes A/S), Cellusoft™(Novozymes A/S), Whitezyme™ (Novozymes A/S), Clazinase™ and Puradax HA™(Genencor International Inc.), and KAC-500(B)™ (Kao Corporation).

Mannanases

Suitable mannanases include those of bacterial or fungal origin.Chemically or genetically modified mutants are included. The mannanasemay be an alkaline mannanase of Family 5 or 26. It may be a wild-typefrom Bacillus or Humicola, particularly B. agaradhaerens, B.licheniformis, B. halodurans, B. clausii, or H. insolens. Suitablemannanases are described in WO 1999/064619. A commercially availablemannanase is Mannaway (Novozymes A/S).

Peroxidases/Oxidases:

A peroxidase according to the invention is a peroxidase enzyme comprisedby the enzyme classification EC 1.11.1.7, as set out by the NomenclatureCommittee of the International Union of Biochemistry and MolecularBiology (IUBMB), or any fragment derived therefrom, exhibitingperoxidase activity.

Suitable peroxidases include those of plant, bacterial or fungal origin.Chemically modified or protein engineered mutants are included. Examplesof useful peroxidases include peroxidases from Coprinopsis, e.g., fromC. cinerea (EP 179,486), and variants thereof as those described in WO93/24618, WO 95/10602, and WO 98/15257. Commercially availableperoxidases include Guardzyme™ (Novozymes A/S).

A peroxidase according to the invention also include a haloperoxidaseenzyme, such as chloroperoxidase, bromoperoxidase and compoundsexhibiting chloroperoxidase or bromoperoxidase activity. Haloperoxidasesare classified according to their specificity for halide ions.Chloroperoxidases (E.C. 1.11.1.10) catalyze formation of hypochloritefrom chloride ions.

In an embodiment, the haloperoxidase of the invention is achloroperoxidase. Preferably, the haloperoxidase is a vanadiumhaloperoxidase, i.e., a vanadate-containing haloperoxidase. In apreferred method of the present invention the vanadate-containinghaloperoxidase is combined with a source of chloride ion.

Haloperoxidases have been isolated from many different fungi, inparticular from the fungus group dematiaceous hyphomycetes, such asCaldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C.verruculosa and C. inaequalis, Drechslera, Ulocladium and Botrytis.

Haloperoxidases have also been isolated from bacteria such asPseudomonas, e.g., P. pyrrocinia and Streptomyces, e.g., S.aureofaciens.

In an preferred embodiment, the haloperoxidase is derivable fromCurvularia sp., in particular Curvularia verruculosa or Curvulariainaequalis, such as C. inaequalis CBS 102.42 as described in WO95/27046; or C. verruculosa CBS 147.63 or C. verruculosa CBS 444.70 asdescribed in WO 97/04102; or from Drechslera hartlebii as described inWO 01/79459, Dendryphiella salina as described in WO 01/79458,Phaeotrichoconis crotalarie as described in WO 01/79461, orGeniculosporium sp. as described in WO 01/79460.

An oxidase according to the invention include, in particular, anylaccase enzyme comprised by the enzyme classification EC 1.10.3.2, orany fragment derived therefrom exhibiting laccase activity, or acompound exhibiting a similar activity, such as a catechol oxidase (EC1.10.3.1), an o-aminophenol oxidase (EC 1.10.3.4), or a bilirubinoxidase (EC 1.3.3.5).

Preferred laccase enzymes are enzymes of microbial origin. The enzymesmay be derived from plants, bacteria or fungi (including filamentousfungi and yeasts).

Suitable examples from fungi include a laccase derivable from a strainof Aspergillus, Neurospora, e.g., N. crassa, Podospora, Botrytis,Collybia, Fomes, Lentinus, Pleurotus, Trametes, e.g., T. villosa and T.versicolor, Rhizoctonia, e.g., R. solani, Coprinopsis, e.g., C. cinerea,C. comatus, C. friesii, and C. plicatilis, Psathyrella, e.g., P.condelleana, Panaeolus, e.g., P. papilionaceus, Myceliophthora, e.g., M.thermophila, Schytalidium, e.g., S. thermophilum, Polyporus, e.g., P.pinsitus, Phlebia, e.g., P. radiata (WO 92/01046), or Coriolus, e.g., C.hirsutus (JP 2238885).

Suitable examples from bacteria include a laccase derivable from astrain of Bacillus. A laccase derived from Coprinopsis or Myceliophthorais preferred; in particular a laccase derived from Coprinopsis cinerea,as disclosed in WO 97/08325; or from Myceliophthora thermophila, asdisclosed in WO 95/33836.

Proteases

Suitable proteases include those of bacterial, fungal, plant, viral oranimal origin e.g. vegetable or microbial origin. Microbial origin ispreferred. Chemically modified or protein engineered mutants areincluded. It may be an alkaline protease, such as a serine protease or ametalloprotease. A serine protease may for example be of the 51 family,such as trypsin, or the S8 family such as subtilisin. A metalloproteasesprotease may for example be a thermolysin from e.g. family M4 or othermetalloprotease such as those from M5, M7 or M8 families.

The term “subtilases” refers to a sub-group of serine protease accordingto Siezen et al., Protein Engng. 4 (199I) 719-737 and Siezen et al.Protein Science 6 (1997) 501-523. Serine proteases are a subgroup ofproteases characterized by having a serine in the active site, whichforms a covalent adduct with the substrate. The subtilases may bedivided into 6 sub-divisions, i.e. the Subtilisin family, the Thermitasefamily, the Proteinase K family, the Lantibiotic peptidase family, theKexin family and the Pyrolysin family.

Examples of subtilases are those derived from Bacillus such as Bacilluslentus, Bacillus alkalophilus, Bacillus subtilis, Bacillusamyloliquefaciens, Bacillus pumilus and Bacillus gibsonii described in;U.S. Pat. No. 7,262,042 and WO09/021867, and Subtilisin lentus,Subtilisin Novo, subtilisin Carlsberg, Bacillus licheniformis,subtilisin BPN′, subtilisin 309, subtilisin 147 and subtilisin 168 ande.g. protease PD138 described in (WO93/18140). Other useful proteasesmay be those described in WO01/016285 and WO02/016547. Examples oftrypsin-like proteases are trypsin (e.g. of porcine or bovine origin)and the Fusarium protease described in WO94/25583 and WO05/040372, andthe chymotrypsin proteases derived from Cellumonas described inWO05/052161 and WO05/052146.

A further preferred protease is the alkaline protease from Bacilluslentus DSM 5483, as described for example in WO95/2322I, and variantsthereof which are described in WO92/21760, WO95/2322I, EP1921147 andEP1921148.

Examples of metalloproteases are the neutral metalloprotease asdescribed in WO07/044993 (Proctor & Gamble/Genencor Int.) such as thosederived from Bacillus amyloliquefaciens.

Examples of useful proteases are the variants described in: WO89/06279WO92/19729, WO96/034946, WO98/20115, WO98/20116, WO99/011768,WO01/44452, WO03/006602, WO04/03186, WO04/041979, WO07/006305,WO11/036263, WO11/036264, especially the variants with substitutions inone or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59,60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126,127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189,193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239,246, 255, 256, 268 and 269 wherein the positions correspond to thepositions of the Bacillus lentus protease shown in SEQ ID NO 1 of WO2016/001449. More preferred the protease variants may comprise one ormore of the mutations selected from the group consisting of: S3T, V4I,S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E,V66A, N74D, S85R, A96S, S97G, S97D, 597A, S99E, S99D, S99G, S99M, S99N,S99R, S99H, S101A, V102I, V102Y, V102N, S104A, G116V, G116R, H118D,H118N, A120S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E,Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D,V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L,Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D T268A and R269H.The protease variants are preferably variants of the Bacillus lentusprotease shown in SEQ ID NO 1 of WO2016/001449, the Bacillusamylolichenifaciens protease (BPN′) shown in SEQ ID NO 2 ofWO2016/001449. The protease variants preferably have at least 80%sequence identity to SEQ ID NO 1 or SEQ ID NO 2 of WO 2016/001449.

A protease variant comprising a substitution at one or more positionscorresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO: 1 ofWO2004/067737, wherein said protease variant has a sequence identity ofat least 75% but less than 100% to SEQ ID NO: 1 of WO2004/067737.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutation S99D, wherein position numberscorrespond to positions of the polypeptide of SEQ ID NO: 20, for examplea variant having at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97% or at least 98% sequence identity to SEQ IDNO: 19. In one embodiment, the protease comprises or consists of thepolypeptide of SEQ ID NO: 19 with the mutation S99D.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutation S99E, wherein position numberscorrespond to positions of the polypeptide of SEQ ID NO: 20, for examplea variant having at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97% or at least 98% sequence identity to SEQ IDNO: 19. In one embodiment, the protease comprises or consists of thepolypeptide of SEQ ID NO: 19 with the mutation S99E.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutations Y167A+R170S+A194P, wherein positionnumbers correspond to positions of the polypeptide of SEQ ID NO: 20, forexample a variant having at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97% or at least 98% sequence identityto SEQ ID NO: 19. In one embodiment, the protease comprises or consistsof the polypeptide of SEQ ID NO: 19 with the mutationsY167A+R170S+A194P.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutationsS9E+N43R+N76D+V205I+Q206L+Y209W+S259D+N261W+L262E, wherein positionnumbers correspond to positions of the polypeptide of SEQ ID NO: 20, forexample a variant having at least 80%, at least 85%, at least 90% or atleast 95% sequence identity to SEQ ID NO: 19. In one embodiment, theprotease comprises or consists of the polypeptide of SEQ ID NO: 19 withthe mutations S9E+N43R+N76D+V205I+Q206L+Y209W+S259D+N261W+L262E.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutationsS3T+V4I+S99D+S101R+S103A+V104I+G160S+V199M+V205I+L217D, wherein positionnumbers correspond to positions of the polypeptide of SEQ ID NO: 20, forexample a variant having at least 80%, at least 85%, at least 90% or atleast 95% sequence identity to SEQ ID NO: 19. In one embodiment, theprotease comprises or consists of the polypeptide of SEQ ID NO: 19 withthe mutations S3T+V4I+S99D+S101R+S103A+V104I+G160S+V199M+V205I+L217D.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutationsS3T+V4I+S99D+S101E+S103A+V104I+G160S+V205I, wherein position numberscorrespond to positions of the polypeptide of SEQ ID NO: 20, for examplea variant having at least 80%, at least 85%, at least 90% or at least95% sequence identity to SEQ ID NO: 19. In one embodiment, the proteasecomprises or consists of the polypeptide of SEQ ID NO: 19 with themutations S3T+V4I+S99D+S101E+S103A+V104I+G160S+V205I.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutations S99D+S101E+S103A+V104I+G160S, whereinposition numbers correspond to positions of the polypeptide of SEQ IDNO: 20, for example a variant having at least 80%, at least 85%, atleast 90%, at least 95% or at least 96% sequence identity to SEQ ID NO:19. In one embodiment, the protease comprises or consists of thepolypeptide of SEQ ID NO: 19 with the mutationsS99D+S101E+S103A+V104I+G160S.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutationsS99D+S101E+S103A+V104I+S156D+G160S+L262E, wherein position numberscorrespond to positions of the polypeptide of SEQ ID NO: 20, for examplea variant having at least 80%, at least 85%, at least 90% or at least95% sequence identity to SEQ ID NO: 19. In one embodiment, the proteasecomprises or consists of the polypeptide of SEQ ID NO: 19 with themutations S99D+S101E+S103A+V104I+S156D+G160S+L262E.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 19 comprising the mutations S87N+S101G+V104N, wherein positionnumbers correspond to positions of the polypeptide of SEQ ID NO: 20, forexample a variant having at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97% or at least 98% sequence identityto SEQ ID NO: 19. In one embodiment, the protease comprises or consistsof the polypeptide of SEQ ID NO: 19 with the mutations S87N+S101G+V104N.

In one embodiment, the protease comprises or consists of the polypeptideof SEQ ID NO: 20.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 20 comprising the mutation Y217L, for example a variant having atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97% or at least 98% sequence identity to SEQ ID NO: 20. In oneembodiment, the protease comprises or consists of the polypeptide of SEQID NO: 20 with the mutation Y217L.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 20 comprising the mutations S24G+S53G+S78N+S101N+G128S+Y217Q, forexample a variant having at least 80%, at least 85%, at least 90%, atleast 95% or at least 96% sequence identity to SEQ ID NO: 20. In oneembodiment, the protease comprises or consists of the polypeptide of SEQID NO: 20 with the mutations S24G+S53G+S78N+S101N+G128S+Y217Q.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 20 comprising the mutations S24G+S53G+S78N+S101N+G128A+Y217Q, forexample a variant having at least 80%, at least 85%, at least 90%, atleast 95% or at least 96% sequence identity to SEQ ID NO: 20. In oneembodiment, the protease comprises or consists of the polypeptide of SEQID NO: 20 with the mutations S24G+S53G+S78N+S101N+G128A+Y217Q.

In one embodiment, the protease comprises or consists of the polypeptideof SEQ ID NO: 21.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 21 having at least 80%, at least 85%, at least 90% or at least95% sequence identity to SEQ ID NO: 21. The protease may e.g. be avariant of the polypeptide of SEQ ID NO: 21 comprising one or moremutations selected from the group consisting of S27K, N109K, S111E,S171E, S173P, G174K, S175P, F180Y, G182A, L184F, Q198E, N199K and T297P,for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 or all of saidmutations.

In one embodiment, the protease is a variant of the polypeptide of SEQID NO: 21 comprising the mutations S27K+N109K+S111E+517IE+S173P+G174K+S175P+F180Y+G182A+L184F+Q198E+N199K+T297P, for example avariant having at least 80%, at least 85%, at least 90% or at least 95%sequence identity to SEQ ID NO: 21. In one embodiment, the proteasecomprises or consists of the polypeptide of SEQ ID NO: 21 with themutationsS27K+N109K+S111E+S171E+S173P+G174K+S175P+F180Y+G182A+L184F+Q198E+N199K+T297P.

Suitable commercially available protease enzymes include those soldunder the trade names Alcalase®, Duralase™, Durazym™, Relase®, Relase®Ultra, Savinase®, Savinase® Ultra, Primase®, Polarzyme®, Kannase®,Liquanase®, Liquanase® Ultra, Ovozyme®, Coronase®, Coronase® Ultra,Blaze®, Blaze Evity® 100T, Blaze Evity® 125T, Blaze Evity® 150T,Neutrase®, Everlase®, Esperase®, Novozymes Progress®, NovozymesProgress® Uno and Novozymes Progress® Excel (Novozymes A/S), those soldunder the tradename Maxatase®, Maxacal®, Maxapem®, Purafect Ox®,Purafect OxP®, Puramax®, FN2®, FN3®, FN4®, Excellase®, Excellenz P1000™,Excellenz P1250™, Eraser®, Preferenz P100™, Purafect Prime®, PreferenzP110™, Effectenz P1000™, Purafect®™, Effectenz P1050™, Purafect Ox® ™,Effectenz P2000™, Purafast®, Properase®, Opticlean® and Optimase®(Danisco/DuPont), Axapem™ (Gist-Brocases N.V.), BLAP (sequence shown inFIG. 29 of U.S. Pat. No. 5,352,604) and variants hereof (Henkel AG) andKAP (Bacillus alkalophilus subtilisin) from Kao.

Nucleases

Suitable nucleases include deoxyribonucleases (DNases) and ribonucleases(RNases) which are any enzyme that catalyzes the hydrolytic cleavage ofphosphodiester linkages in the DNA or RNA backbone respectively, thusdegrading DNA and RNA. There are two primary classifications based onthe locus of activity. Exonucleases digest nucleic acids from the ends.Endonucleases act on regions in the middle of target molecules. Thenuclease is preferably a DNase, which is preferable is obtainable from amicroorganism, preferably a fungi or bacterium. In particular, a DNasewhich is obtainable from a species of Bacillus is preferred; inparticular a DNase which is obtainable from Bacillus cibi, Bacillussubtilis or Bacillus licheniformis is preferred. Examples of such DNasesare described in WO 2011/098579, WO2014/087011 and WO2017/060475.Particularly preferred is also a DNase obtainable from a species ofAspergillus; in particular a DNase which is obtainable from Aspergillusoryzae, such as a DNase described in WO 2015/155350. The detergentenzyme(s) may be included in a detergent composition by adding separateadditives containing one or more enzymes, or by adding a combinedadditive comprising all of these enzymes. A detergent additive of theinvention, i.e., a separate additive or a combined additive, can beformulated, for example, as a granulate, liquid, slurry, etc. Preferreddetergent additive formulations are granulates, in particularnon-dusting granulates as described above, liquids, in particularstabilized liquids, or slurries.

Lipases and Cutinases:

Suitable lipases and cutinases include those of bacterial or fungalorigin. Chemically modified or protein engineered mutant enzymes areincluded. Examples include lipase from Thermomyces, e.g. from T.lanuginosus (previously named Humicola lanuginosa) as described inEP258068 and EP305216, cutinase from Humicola, e.g. H. insolens(WO96/13580), lipase from strains of Pseudomonas (some of these nowrenamed to Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes(EP218272), P. cepacia (EP331376), P. sp. strain SD705 (WO95/06720 &WO96/27002), P. wisconsinensis (WO96/12012), GDSL-type Streptomyceslipases (WO10/065455), cutinase from Magnaporthe grisea (WO10/107560),cutinase from Pseudomonas mendocina (U.S. Pat. No. 5,389,536), lipasefrom Thermobifida fusca (WO11/084412), Geobacillus stearothermophiluslipase (WO11/084417), lipase from Bacillus subtilis (WO11/084599), andlipase from Streptomyces griseus (WO11/150157) and S. pristinaespiralis(WO12/137147).

Other examples are lipase variants such as those described in EP407225,WO92/05249, WO94/01541, WO94/25578, WO95/14783, WO95/30744, WO95/35381,WO95/22615, WO96/00292, WO97/04079, WO97/07202, WO00/34450, WO00/60063,WO01/92502, WO07/87508 and WO09/109500.

Preferred commercial lipase products include include Lipolase™, Lipex™;Lipolex™ and Lipoclean™ (Novozymes A/S), Lumafast (originally fromGenencor) and Lipomax (originally from Gist-Brocades).

Still other examples are lipases sometimes referred to asacyltransferases or perhydrolases, e.g. acyltransferases with homologyto Candida antarctica lipase A (WO10/111143), acyltransferase fromMycobacterium smegmatis (WO05/56782), perhydrolases from the CE 7 family(WO09/67279), and variants of the M. smegmatis perhydrolase inparticular the S54V variant used in the commercial product Gentle PowerBleach from Huntsman Textile Effects Pte Ltd (WO10/100028).

Amylases:

Suitable amylases which can be used together with the compositions ofthe invention may be an alpha-amylase or a glucoamylase and may be ofbacterial or fungal origin. Chemically modified or protein engineeredmutants are included. Amylases include, for example, alpha-amylasesobtained from Bacillus, e.g., a special strain of Bacilluslicheniformis, described in more detail in GB 1,296,839.

Suitable amylases include amylases having SEQ ID NO: 2 in WO 95/10603 orvariants having 90% sequence identity to SEQ ID NO: 3 thereof. Preferredvariants are described in WO 94/02597, WO 94/18314, WO 97/43424 and SEQID NO: 4 of WO 99/019467, such as variants with substitutions in one ormore of the following positions: 15, 23, 105, 106, 124, 128, 133, 154,156, 178, 179, 181, 188, 190, 197, 201, 202, 207, 208, 209, 211, 243,264, 304, 305, 391, 408, and 444.

Different suitable amylases include amylases having SEQ ID NO: 6 in WO02/010355 or variants thereof having 90% sequence identity to SEQ ID NO:6. Preferred variants of SEQ ID NO: 6 are those having a deletion inpositions 181 and 182 and a substitution in position 193.

Other amylases which are suitable are hybrid alpha-amylase comprisingresidues 1-33 of the alpha-amylase derived from B. amyloliquefaciensshown in SEQ ID NO: 6 of WO 2006/066594 and residues 36-483 of the B.licheniformis alpha-amylase shown in SEQ ID NO: 4 of WO 2006/066594 orvariants having 90% sequence identity thereof. Preferred variants ofthis hybrid alpha-amylase are those having a substitution, a deletion oran insertion in one of more of the following positions: G48, T49, G107,H156, A181, N190, M197, I20I, A209 and Q264. Most preferred variants ofthe hybrid alpha-amylase comprising residues 1-33 of the alpha-amylasederived from B. amyloliquefaciens shown in SEQ ID NO: 6 of WO2006/066594 and residues 36-483 of SEQ ID NO: 4 are those having thesubstitutions:

M197T;

H156Y+A181T+N190F+A209V+Q264S; or

G48A+T491+G107A+H156Y+A181T+N190F+120I F+A209V+Q2645.

Further amylases which are suitable are amylases having SEQ ID NO: 6 inWO 99/019467 or variants thereof having 90% sequence identity to SEQ IDNO: 6. Preferred variants of SEQ ID NO: 6 are those having asubstitution, a deletion or an insertion in one or more of the followingpositions: R181, G182, H183, G184, N195, 1206, E212, E216 and K269.Particularly preferred amylases are those having deletion in positionsR181 and G182, or positions H183 and G184.

Additional amylases which can be used are those having SEQ ID NO: 1, SEQID NO: 3, SEQ ID NO: 2 or SEQ ID NO: 7 of WO 96/023873 or variantsthereof having 90% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 3 or SEQ ID NO: 7. Preferred variants of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO: 3 or SEQ ID NO: 7 are those having a substitution, adeletion or an insertion in one or more of the following positions: 140,181, 182, 183, 184, 195, 206, 212, 243, 260, 269, 304 and 476, using SEQID 2 of WO 96/023873 for numbering. More preferred variants are thosehaving a deletion in two positions selected from 181, 182, 183 and 184,such as 181 and 182, 182 and 183, or positions 183 and 184. Mostpreferred amylase variants of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 7are those having a deletion in positions 183 and 184 and a substitutionin one or more of positions 140, 195, 206, 243, 260, 304 and 476.

Other amylases which can be used are amylases having SEQ ID NO: 2 of WO08/153815, SEQ ID NO: 10 in WO 01/66712 or variants thereof having 90%sequence identity to SEQ ID NO: 2 of WO 08/153815 or 90% sequenceidentity to SEQ ID NO: 10 in WO 01/66712. Preferred variants of SEQ IDNO: 10 in WO 01/66712 are those having a substitution, a deletion or aninsertion in one of more of the following positions: 176, 177, 178, 179,190, 201, 207, 211 and 264.

Further suitable amylases are amylases having SEQ ID NO: 2 of WO09/061380 or variants having 90% sequence identity to SEQ ID NO: 2thereof. Preferred variants of SEQ ID NO: 2 are those having atruncation of the C-terminus and/or a substitution, a deletion or aninsertion in one of more of the following positions: Q87, Q98, S125,N128, T131, T165, K178, R180, S181, T182, G183, M201, F202, N225, S243,N272, N282, Y305, R309, D319, Q320, Q359, K444 and G475. More preferredvariants of SEQ ID NO: 2 are those having the substitution in one ofmore of the following positions: Q87E, R, Q98R, S125A, N128C, T131I,T165I, K178L, T182G, M201L, F202Y, N225E, R, N272E, R, S243Q, A, E, D,Y305R, R309A, Q320R, Q359E, K444E and G475K and/or deletion in positionR180 and/or S181 or of T182 and/or G183. Most preferred amylase variantsof SEQ ID NO: 2 are those having the substitutions:

N128C+K178L+T182G+Y305R+G475K;

N128C+K178L+T182G+F202Y+Y305R+D319T+G475K;

S125A+N128C+K178L+T182G+Y305R+G475K; or

S125A+N128C+T131I+T165I+K178L+T182G+Y305R+G475K wherein the variants areC-terminally truncated and optionally further comprises a substitutionat position 243 and/or a deletion at position 180 and/or position 181.

Further suitable amylases are amylases having SEQ ID NO: 1 of WO13184577or variants having 90% sequence identity to SEQ ID NO: 1 thereof.Preferred variants of SEQ ID NO: 1 are those having a substitution, adeletion or an insertion in one of more of the following positions:K176, R178, G179, T180, G181, E187, N192, M199, 1203, S241, R458, T459,D460, G476 and G477. More preferred variants of SEQ ID NO: 1 are thosehaving the substitution in one of more of the following positions:K176L, E187P, N192FYH, M199L, 1203YF, S241QADN, R458N, T459S, D460T,G476K and G477K and/or deletion in position R178 and/or S179 or of T180and/or G181. Most preferred amylase variants of SEQ ID NO: 1 are thosehaving the substitutions:

E187P+1203Y+G476K

E187P+1203Y+R458N+T459S+D460T+G476K

wherein the variants optionally further comprises a substitution atposition 241 and/or a deletion at position 178 and/or position 179.

Further suitable amylases are amylases having SEQ ID NO: 1 of WO10104675or variants having 90% sequence identity to SEQ ID NO: 1 thereof.Preferred variants of SEQ ID NO: 1 are those having a substitution, adeletion or an insertion in one of more of the following positions: N21,D97, V128 K177, R179, S180, 118I, G182, M200, L204, E242, G477 and G478.More preferred variants of SEQ ID NO: 1 are those having thesubstitution in one of more of the following positions: N21D, D97N,V1281 K177L, M200L, L204YF, E242QA, G477K and G478K and/or deletion inposition R179 and/or S180 or of I18I and/or G182. Most preferred amylasevariants of SEQ ID NO: 1 are those having the substitutions:

N21D+D97N+V1281

wherein the variants optionally further comprise a substitution atposition 200 and/or a deletion at position 180 and/or position 181.

Other suitable amylases are the alpha-amylase having SEQ ID NO: 12 inWO01/66712 or a variant having at least 90% sequence identity to SEQ IDNO: 12. Preferred amylase variants are those having a substitution, adeletion or an insertion in one of more of the following positions ofSEQ ID NO: 12 in WO01/66712: R28, R118, N174; R181, G182, D183, G184,G186, W189, N195, M202, Y298, N299, K302, S303, N306, R310, N314; R320,H324, E345, Y396, R400, W439, R444, N445, K446, Q449, R458, N471, N484.Particular preferred amylases include variants having a deletion of D183and G184 and having the substitutions R118K, N195F, R320K and R458K, anda variant additionally having substitutions in one or more positionselected from the group: M9, G149, G182, G186, M202, T257, Y295, N299,M323, E345 and A339, most preferred a variant that additionally hassubstitutions in all these positions.

Other examples are amylase variants such as those described inWO2011/09853I, WO2013/001078 and WO2013/001087.

Commercially available amylases are Amplify Prime™, Atlantic™, Arctic™,Everest™ Duramyl™, Termamyl™, Fungamyl™, Stainzyme™, Stainzyme Plus™,Natalase™, Liquozyme X and BAN™ (from Novozymes A/S), and Rapidase™,Purastar™/Effectenz™, Powerase, Preferenz S1000, Preferenz S100 andPreferenz S110 (from Genencor International Inc./DuPont).

The detergent enzyme(s) may be included in the detergent compositionaccording to the invention by adding separate additives containing oneor more enzymes, or by adding a combined additive comprising all ofthese enzymes. A detergent additive, i.e., a separate additive or acombined additive, may be formulated, for example, as a granulate,liquid, slurry, etc. Preferred detergent additive formulations aregranulates, in particular non-dusting granulates, liquids, in particularstabilized liquids, or slurries.

Non-dusting granulates may be produced, e.g., as disclosed in U.S. Pat.Nos. 4,106,991 and 4,661,452 and may optionally be coated by methodsknown in the art. Examples of waxy coating materials are poly(ethyleneoxide) products (polyethyleneglycol, PEG) with mean molar weights of1000 to 20000; ethoxylated nonylphenols having from 16 to 50 ethyleneoxide units; ethoxylated fatty alcohols in which the alcohol containsfrom 12 to 20 carbon atoms and in which there are 15 to 80 ethyleneoxide units; fatty alcohols; fatty acids; and mono- and di- andtriglycerides of fatty acids. Examples of film-forming coating materialssuitable for application by fluid bed techniques are given in GB1483591. Liquid enzyme preparations may, for instance, be stabilized byadding a polyol such as propylene glycol, a sugar or sugar alcohol,lactic acid or boric acid according to established methods. Protectedenzymes may be prepared according to the method disclosed in EP 238,216.

Microorgansims

The detergent additive as well as the detergent composition may alsocomprise one or more microorganisms, such as one or more fungi, yeast,or bacteria.

In an embodiment, the one or more microorganisms are dehydrated (forexample by lyophilization) bacteria or yeast, such as a strain ofLactobacillus.

In another embodiment, the microrganisms are one or more microbialspores (as opposed to vegetative cells), such as bacterial spores; orfungal spores, conidia, hypha. Preferably, the one or more spores areBacillus endospores; even more preferably the one or more spores areendospores of Bacillus subtilis, Bacillus licheniformis, Bacillusamyloliquefaciens, or Bacillus megaterium.

The microrganisms may be included in the detergent composition oradditive in the same way as enzymes (see above).

It is at present contemplated that in the detergent compositions anyenzyme, in particular the alpha amylase polypeptides of the invention,may be added in an amount corresponding to 0.01-100 mg of enzyme proteinper liter of wash liquor, preferably 0.05-5 mg of enzyme protein perliter of wash liquor, in particular 0.1-1 mg of enzyme protein per literof wash liquor.

The alpha amylase polypeptides of the invention may additionally beincorporated in the detergent formulations disclosed in WO 2006/002643,which is hereby incorporated as reference.

Formulation of Detergent Products

The detergent composition of the invention may be in any convenientform, e.g., a bar, a homogenous tablet, a tablet having two or morelayers, a pouch having one or more compartments, a regular or compactpowder, a granule, a paste, a gel, or a regular, compact or concentratedliquid.

A detergent composition according to the invention may be formulated,for example, as a hand or machine laundry detergent compositionincluding a laundry additive composition suitable for pre-treatment ofstained fabrics and a rinse added fabric softener composition, or beformulated as a detergent composition for use in general household hardsurface cleaning operations, or be formulated for hand or machinedishwashing operations.

Thus, in one embodiment, the detergent composition according to thepresent invention is a liquid laundry detergent composition, a powderlaundry detergent composition, a liquid dishwash detergent composition,or a powder dishwash detergent composition. In an embodiment, thecomposition is a liquid or powder automatic dishwashing (ADW) detergentcomposition; or a liquid manual dishwashing detergent composition.

Pouches can be configured as single or multicompartments. It can be ofany form, shape and material which is suitable for hold the composition,e.g. without allowing the release of the composition to release of thecomposition from the pouch prior to water contact. The pouch is madefrom water soluble film which encloses an inner volume. Said innervolume can be divided into compartments of the pouch. Preferred filmsare polymeric materials preferably polymers which are formed into a filmor sheet. Preferred polymers, copolymers or derivates thereof areselected polyacrylates, and water soluble acrylate copolymers, methylcellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose,hydroxyethyl cellulose, hydroxypropyl methyl cellulose, malto dextrin,poly methacrylates, most preferably polyvinyl alcohol copolymers and,hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymerin the film for example PVA is at least about 60%. Preferred averagemolecular weight will typically be about 20,000 to about 150,000. Filmscan also be of blended compositions comprising hydrolytically degradableand water soluble polymer blends such as polylactide and polyvinylalcohol (known under the Trade reference M8630 as sold by MonoSol LLC,Indiana, USA) plus plasticisers like glycerol, ethylene glycerol,propylene glycol, sorbitol and mixtures thereof. The pouches cancomprise a solid laundry cleaning composition or part components and/ora liquid cleaning composition or part components separated by the watersoluble film. The compartment for liquid components can be different incomposition than compartments containing solids: US2009/0011970 A1.

Detergent ingredients can be separated physically from each other bycompartments in water dissolvable pouches or in different layers oftablets. Thereby negative storage interaction between components can beavoided. Different dissolution profiles of each of the compartments canalso give rise to delayed dissolution of selected components in the washsolution.

A liquid or gel detergent, which is not unit dosed, may be aqueous,typically containing at least 20% by weight and up to 95% water, such asup to about 70% water, up to about 65% water, up to about 55% water, upto about 45% water, up to about 35% water. Other types of liquids,including without limitation, alkanols, amines, diols, ethers andpolyols may be included in an aqueous liquid or gel. An aqueous liquidor gel detergent may contain from 0-30% organic solvent.

A liquid or gel detergent may be non-aqueous.

Granular Detergent Formulations

A granular detergent may be formulated as described in WO09/092699,EP170524I, EP1382668, WO07/001262, U.S. Pat. No. 6,472,364, WO04/074419or WO09/102854. Other useful detergent formulations are described inWO09/124162, WO09/124163, WO09/117340, WO09/11734I, WO09/117342,WO09/072069, WO09/063355, WO09/132870, WO09/121757, WO09/112296,WO09/112298, WO09/103822, WO09/087033, WO09/050026, WO09/047125,WO09/047126, WO09/047127, WO09/047128, WO09/021784, WO09/010375,WO09/000605, WO09/122125, WO09/095645, WO09/040544, WO09/040545,WO09/024780, WO09/004295, WO09/004294, WO09/121725, WO09/11539I,WO09/115392, WO09/074398, WO09/074403, WO09/06850I, WO09/065770,WO09/021813, WO09/030632, and WO09/015951.

WO2011025615, WO2011016958, WO2011005803, WO2011005623, WO2011005730,WO2011005844, WO2011005904, WO2011005630, WO2011005830, WO2011005912,WO2011005905, WO2011005910, WO2011005813, WO2010135238, WO2010120863,WO2010108002, WO2010111365, WO2010108000, WO2010107635, WO2010090915,WO2010033976, WO2010033746, WO2010033747, WO2010033897, WO2010033979,WO2010030540, WO201003054I, WO2010030539, WO2010024467, WO2010024469,WO2010024470, WO201002516I, WO2010014395, WO2010044905,

WO2010145887, WO2010142503, WO201012205I, WO201010286I, WO2010099997,WO2010084039, WO2010076292, WO2010069742, WO2010069718, WO2010069957,WO2010057784, WO2010054986, WO2010018043, WO2010003783, WO2010003792,

WO2011023716, WO2010142539, WO2010118959, WO2010115813, WO2010105942,WO201010596I, WO2010105962, WO2010094356, WO2010084203, WO2010078979,WO2010072456, WO2010069905, WO2010076165, WO2010072603, WO2010066486,WO201006663I, WO2010066632, WO2010063689, WO201006082I, WO2010049187,WO2010031607, WO2010000636.

Formulation of Enzyme in Co-Granule

The enzyme of the invention may be formulated as a granule for exampleas a co-granule that combines one or more enzymes. Each enzyme will thenbe present in more granules securing a more uniform distribution ofenzymes in the detergent. This also reduces the physical segregation ofdifferent enzymes due to different particle sizes. Methods for producingmulti-enzyme co-granulates for the detergent industry are disclosed inthe IP.com disclosure IPCOM000200739D.

Another example of formulation of enzymes by the use of co-granulatesare disclosed in WO 2013/188331, which relates to a detergentcomposition comprising (a) a multi-enzyme co-granule; (b) less than 10wt zeolite (anhydrous basis); and (c) less than 10 wt phosphate salt(anhydrous basis), wherein said enzyme co-granule comprises from 10 to98 wt % moisture sink component and the composition additionallycomprises from 20 to 80 wt % detergent moisture sink component.

WO 2013/188331 also relates to a method of treating and/or cleaning asurface, preferably a fabric surface comprising the steps of (i)contacting said surface with the detergent composition as claimed anddescribed herein in an aqueous wash liquor, (ii) rinsing and/or dryingthe surface.

The multi-enzyme co-granule may comprise an enzyme of the invention and(a) one or more enzymes selected from the group consisting of lipases,cellulases, xyloglucanases, perhydrolases, peroxidases, lipoxygenases,laccases, hemicellulases, proteases, cellobiose dehydrogenases,xylanases, phospholipases, esterases, cutinases, pectinases, mannanases,pectate lyases, keratinases, reductases, oxidases, phenoloxidases,ligninases, pullulanases, tannases, pentosanases, lichenases glucanases,arabinosidases, hyaluronidase, chondroitinase, second amylases, andmixtures thereof.

Uses

The present invention is also directed to methods for using thealpha-amylase variants. The alpha-amylase variants of the invention areuseful in detergent compositions, laundry washing, dishwashing and/orcleaning processes.

In one embodiment, the invention relates the use of variants of thepresent invention in detergent compositions, for use in cleaninghard-surfaces, such as dish wash, or in laundering or for stain removal.In another embodiment, the invention relates to the use of analpha-amylase variant according to the invention in a cleaning processsuch as laundry or hard surface cleaning including, but not limited to,dish wash and industrial cleaning. Thus, in one embodiment, theinvention relates to the use of a variant comprising an modification inone or more positions corresponding to the positions H1, H2, G7, I9,Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81,E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481 of SEQ ID NO: 2 in a cleaning process such as laundry orhard surface cleaning including dish wash and industrial cleaning.

In a particular embodiment, the invention relates to the use of avariant comprising a) a substitution and/or deletion of two or morepositions in the parent alpha-amylase said positions corresponding topositions R181, G182, D183, and G184 of the polypeptide of SEQ ID NO: 2;and

b) an modification at one or more positions corresponding to positionsH1, H2, G7, I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59,A60, L63, T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118,Q125, R127, E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149,R158, Y160, D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176,Y178, R181, G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210,E212, T227, L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269,N270, L272, G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298,Q299, N302, S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320,S323, F328, G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395,R400, D406, W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466,W469, F473, G476 and V481 of SEQ ID NO: 2, in a cleaning process such aslaundry or hard surface cleaning including dish wash and industrialcleaning.

The soils and stains that are important for cleaning are composed ofmany different substances, and a range of different enzymes, all withdifferent substrate specificities, have been developed for use indetergents both in relation to laundry and hard surface cleaning, suchas dishwashing. These enzymes are considered to provide an enzymedetergency benefit, since they specifically improve stain removal in thecleaning process that they are used in, compared to the same processwithout enzymes. Stain removing enzymes that are known in the artinclude enzymes such as proteases, second amylases, lipases, cutinases,cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases,xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases.

In one aspect, the present invention relates to the use of a variant asdescribed herein, or to the use of a composition comprising the variant,in a domestic or industrial cleaning process.

In one aspect, the present invention relates to the use of a variant asdescribed herein, or to the use of a composition comprising the variantfor cleaning of fabric, for example laundry.

In one aspect, the present invention relates to the use of a variant asdescribed herein, or to the use of a composition comprising the variantfor cleaning of ceramic, plastic or glass material, for exampledishwashing.

In one aspect, the invention relates to a laundering process which maybe for household laundering as well as industrial laundering.Furthermore, the invention relates to a process for the laundering oftextiles (e.g. fabrics, garments, cloths etc.) where the processcomprises treating the textile with a washing solution containing adetergent composition of the present invention. The laundering can forexample be carried out using a household or an industrial washingmachine or be carried out by hand using a detergent composition of theinvention.

In one aspect, the invention relates to a dish wash process, includingADW; or hard surface cleaning, which may be for household cleaning aswell as industrial cleaning. Furthermore, the invention relates to aprocess for dish wash or hard surface cleaning, where the processcomprises treating the dishes or hard surfaces with a washing solutioncomprising a detergent composition of the present invention. The dishwash or hard surface cleaning can for example be carried out using ahousehold dish washing machine or be carried out by hand using adetergent composition of the invention.

Methods of Use

The invention provides a use of a detergent composition in a domestic orindustrial cleaning process. A cleaning process may for example be adishwashing process, such as automated dishwashing; a laundry process;or cleaning of hard surfaces such as bathroom tiles, floors, table tops,drains, sinks and washbasins.

Dishwashing

An automated dishwashing process may comprise the following steps:

a. Exposing dishware to an aqueous wash liquor comprising a detergentcomposition;

b. Completing at least one wash cycle; and

c. Optionally rinsing and drying the dishware.

Thus, the invention provides a method of dishwashing in an automaticdishwashing machine using a detergent composition as described herein,comprising the steps of adding said detergent composition in a detergentcomposition compartment in said automatic dishwashing machine, andreleasing said detergent composition during a main-wash cycle.

The compositions may be employed at concentrations from about 1000-8000ppm in the wash liquor, such as 2000-6000 ppm in the wash liquor. Thehardness of the wash liquor may be 3-30° dH. The pH of the wash liquormay be 3-11, such as 7-11.

The temperature of the wash liquor when used may be in the range of10-70° C. For example the temperature of the wash liquor can be in therange of 15-60° C., in the range of 20-50° C., in the range of 25-50°C., in the range of 30-45° C., in the range of 35-40° C., in the rangeof 35-55° C., or in the range of 40-50° C.

The temperature may vary throughout the wash program. One enzyme may beactivated at one active temperature range and other enzymes may beactivated at another active temperature range differing from the activetemperature range of the first enzyme. For example, one or more washcycles may be carried out at a temperature of 32-38° C. and other washcycles may be carried out at a temperature of 45-55° C. The advantage ofthis is that the single enzymes are allowed to work at their optimaltemperature. The optimal temperature of the enzymes of a detergentcomposition may vary but is typically in the range of 65-70° C. forproteases and in the range of 55-65° C. for amylases. The optimaltemperature may be determined by different assays, such as comparing theactivity over a 15 min period of time in a buffered solution atdifferent temperatures.

During or after completion of a wash cycle the dishware can be rinsedwith water or with water comprising a rinsing aid. The effectiveness ofthe cleaning can be further improved if an acidic rinsing aid is used.The rinsing aid should be capable of lowering the pH below 4 during atleast a period of the rinsing step. The pH may be even further lowerede.g. to below pH 3.5, such as below pH 3, below pH 2.5 or below pH 2.The period of lowering the pH may be at least 1 minute, such as at least2 minutes, at least 3 minutes, at least 4 minutes, at least 5 minutes,at least 6 minutes or at least 7 minutes. The period of lowering the pHmay even be as long as the time period for the full rinsing step.

The ability of lowering the pH during the rinsing step is due to abuffering agent. A buffer with strong buffer capacity at low pH, from pH4 and below should be selected. The buffer capacity should correspond tothe same effect as the pH drop was done with 15 ml 4M HCL/rinse cycle.The ability of lowering the pH during the rinsing step is due to abuffering agent selected from the group consisting of citric acid,acetic acid, potassium dihydrogen phosphate, boric acid, diethylbarbituric acid, Carmody buffer and Britton-Robinson buffer.

The rinsing aid can further improve the cleaning of the dishware byrinsing away any soil released from the dishware during the washingcycle. In addition, the acidic rinsing aid prevents precipitation ofcalcium on the dishware.

Laundering

Laundry processes can for example be household laundering, but it mayalso be industrial laundering. A process for laundering of fabricsand/or garments may be a process comprises treating fabrics with awashing solution containing a detergent composition as described herein.A cleaning process or a textile care process can for example be carriedout in a machine washing process or in a manual washing process.

The fabrics and/or garments subjected to a washing, cleaning or textilecare process may be conventional washable laundry, for example householdlaundry. Preferably, the major part of the laundry is garments andfabrics, including knits, woven, denims, non-woven, felts, yarns, andtowelling. The fabrics may be cellulose based such as naturalcellulosics, including cotton, flax, linen, jute, ramie, sisal or coiror manmade cellulosics (e.g., originating from wood pulp) includingviscose/rayon, ramie, cellulose acetate fibres (tricell), lyocell orblends thereof. The fabrics may also be non-cellulose based such asnatural polyamides including wool, camel, cashmere, mohair, rabbit andsilk or synthetic polymer such as nylon, aramid, polyester, acrylic,polypropylene and spandex/elastane, or blends thereof as well as blendof cellulose based and non-cellulose based fibres.

In one aspect, the present invention relates to a method of launderingin an automatic laundering machine using a detergent composition asdescribed herein, comprising the steps of adding said detergentcomposition in a detergent composition compartment in said automaticlaundering machine, and releasing said detergent composition during amain wash cycle. In another aspect, the present invention relates to amethod of laundering, comprising laundering a garment with a detergentcomposition as described herein, preferably at a temperature of 50° C.or less, or more preferably at a temperature of 45° C. or less, or evenmore preferably at a temperature of 40° C. or less even more preferablyat a temperature of 35° C. or less or even more preferably at atemperature of 30° C. or less, even more preferably at a temperature of25° C. or less or even more preferably at a temperature of 20° C. orless.

These methods include a method for laundering a fabric. The methodcomprises the steps of contacting a fabric to be laundered with acleaning laundry solution comprising a detergent composition. The fabricmay comprise any fabric capable of being laundered in normal consumeruse conditions. The solution preferably has a pH from about 5.5 to about11.5. The compositions may be employed at concentrations from about 100ppm, preferably 500 ppm to about 15,000 ppm in solution. The watertemperatures typically range from about 5° C. to about 95° C., includingabout 10° C., about 15° C., about 20° C., about 25° C., about 30° C.,about 35° C., about 40° C., about 45° C., about 50° C., about 55° C.,about 60° C., about 65° C., about 70° C., about 75° C., about 80° C.,about 85° C. and about 90° C. The water to fabric ratio is typicallyfrom about 1:1 to about 30:1.

In particular embodiments, the washing method is conducted at a degreeof hardness of from about 0° dH to about 30° dH. Under typical Europeanwash conditions, the degree of hardness is about 16° dH, under typicalUS wash conditions about 6° dH, and under typical Asian wash conditions,about 3° dH.

The alpha-amylase variants of this invention possess valuable propertiesallowing for a variety of other industrial applications. For example,alpha-amylase polypeptides of the invention may be used for starchprocesses, in particular starch conversion, especially liquefaction ofstarch (see, e.g., U.S. Pat. No. 3,912,590, EP patent application nos.252 730 and 63 909, WO 99/19467, and WO 96/28567 all references herebyincorporated by reference). Also contemplated are compositions forstarch conversion purposes, which may beside the variant of theinvention also comprise a glucoamylase, pullulanase, and otheralpha-amylases.

Furthermore, alpha-amylase variants of this invention are alsoparticularly useful in the production of sweeteners and ethanol (see,e.g., U.S. Pat. No. 5,231,017 hereby incorporated by reference), such asfuel, drinking and industrial ethanol, from starch or whole grains.

Alpha-amylase variants of the invention may also be useful for desizingof textiles, fabrics and garments (see, e.g., WO 95/21247, U.S. Pat. No.4,643,736, EP 119,920 hereby in corporate by reference), beer making orbrewing, in pulp and paper production.

Starch Conversion

Conventional starch-conversion processes, such as liquefaction andsaccharification processes are described, e.g., in U.S. Pat. No.3,912,590 and EP patent publications Nos. 252,730 and 63,909, herebyincorporated by reference.

In an embodiment the starch conversion process degrading starch to lowermolecular weight carbohydrate components such as sugars or fat replacersincludes a debranching step.

In the case of converting starch into a sugar, the starch isdepolymerized. Such depolymerization processes may consist of apre-treatment step and two or three consecutive process steps, viz. aliquefaction process, a saccharification process and dependent on thedesired end product optionally an isomerization process.

(i) Pre-Treatment of Native Starch

Native starch consists of microscopic granules, which are insoluble inwater at room temperature. When an aqueous starch slurry is heated, thegranules swell and eventually burst, dispersing the starch moleculesinto the solution. During this “gelatinization” process there is adramatic increase in viscosity. As the solids level is 30-40% in atypically industrial process, the starch has to be thinned or“liquefied” so that it can be handled. This reduction in viscosity istoday mostly obtained by enzymatic degradation.

(ii) Liquefaction

During the liquefaction step, the long chained starch is degraded intobranched and linear shorter units (maltodextrins) by an alpha-amylase.The liquefaction process is carried out at 105-110° C. for 5 to 10minutes followed by 1-2 hours at 95° C. The pH lies between 5.5 and 6.2.In order to ensure optimal enzyme stability under these conditions, 1 mMof calcium is added (40 ppm free calcium ions). After this treatment theliquefied starch will have a “dextrose equivalent” (DE) of 10-15.

(iii) Saccharification

After the liquefaction process the maltodextrins are converted intodextrose by addition of a glucoamylase (e.g., AMG) and a debranchingenzyme, such as an isoamylase (U.S. Pat. No. 4,335,208) or a pullulanase(e.g., Promozyme™) (U.S. Pat. No. 4,560,651). Before this step the pH isreduced to a value below 4.5, maintaining the high temperature (above95° C.) to inactivate the liquefying alpha-amylase to reduce theformation of short oligosaccharide called “panose precursors” whichcannot be hydrolyzed properly by the debranching enzyme.

The temperature is lowered to 60° C., and glucoamylase and debranchingenzyme are added. The saccharification process proceeds for 24-72 hours.

Normally, when denaturing the α-amylase after the liquefaction stepabout 0.2-0.5% of the saccharification product is the branchedtrisaccharide 6<2>-alpha-glucosyl maltose (panose) which cannot bedegraded by a pullulanase. If active amylase from the liquefaction stepis present during saccharification (i.e., no denaturing), this level canbe as high as 1-2%, which is highly undesirable as it lowers thesaccharification yield significantly.

(iv) Isomerization

When the desired final sugar product is, e.g., high fructose syrup thedextrose syrup may be converted into fructose. After thesaccharification process the pH is increased to a value in the range of6-8, preferably pH 7.5, and the calcium is removed by ion exchange. Thedextrose syrup is then converted into high fructose syrup using, e.g.,an immmobilized glucoseisomerase (such as Sweetzyme™ IT).

Ethanol Production

In general alcohol production (ethanol) from whole grain can beseparated into 4 main steps

-   -   Milling    -   Liquefaction    -   Saccharification    -   Fermentation

(i) Milling

The grain is milled in order to open up the structure and allowing forfurther processing. Two processes are used wet or dry milling. In drymilling the whole kernel is milled and used in the remaining part of theprocess. Wet milling gives a very good separation of germ and meal(starch granules and protein) and is with a few exceptions applied atlocations where there is a parallel production of syrups.

(ii) Liquefaction

In the liquefaction process the starch granules are solubilized byhydrolysis to maltodextrins mostly of a DP higher than 4. The hydrolysismay be carried out by acid treatment or enzymatically by alpha-amylase.Acid hydrolysis is used on a limited basis. The raw material can bemilled whole grain or a side stream from starch processing.

Enzymatic liquefaction is typically carried out as a three-step hotslurry process. The slurry is heated to between 60-95° C., preferably80-85° C., and the enzyme(s) is (are) added. Then the slurry isjet-cooked at between 95-140° C., preferably 105-125° C., cooled to60-95° C. and more enzyme(s) is (are) added to obtain the finalhydrolysis. The liquefaction process is carried out at pH 4.5-6.5,typically at a pH between 5 and 6. Milled and liquefied grain is alsoknown as mash.

(iii) Saccharification

To produce low molecular sugars DP1-3 that can be metabolized by yeast,the maltodextrin from the liquefaction must be further hydrolyzed. Thehydrolysis is typically done enzymatically by glucoamylases,alternatively alpha-glucosidases or acid alpha-amylases can be used. Afull saccharification step may last up to 72 hours, however, it iscommon only to do a pre-saccharification of typically 40-90 minutes andthen complete saccharification during fermentation (SSF).Saccharification is typically carried out at temperatures from 30-65°C., typically around 60° C., and at pH 4.5.

(iv) Fermentation

Yeast typically from Saccharomyces spp. is added to the mash and thefermentation is ongoing for 24-96 hours, such as typically 35-60 hours.The temperature is between 26-34° C., typically at about 32° C., and thepH is from pH 3-6, preferably around pH 4-5.

Note that the most widely used process is a simultaneoussaccharification and fermentation (SSF) process where there is noholding stage for the saccharification, meaning that yeast and enzyme isadded together. When doing SSF it is common to introduce apre-saccharification step at a temperature above 50° C., just prior tothe fermentation.

(v) Distillation

Following the fermentation the mash is distilled to extract the ethanol.

The ethanol obtained according to the process of the invention may beused as, e.g., fuel ethanol; drinking ethanol, i.e., potable neutralspirits; or industrial ethanol.

(vi) By-Products

Left over from the fermentation is the grain, which is typically usedfor animal feed either in liquid form or dried.

Further details on how to carry out liquefaction, saccharification,fermentation, distillation, and recovering of ethanol are well known tothe skilled person.

According to the process of the invention the saccharification andfermentation may be carried out simultaneously or separately.

Pulp and Paper Production

Alkaline alpha-amylase polypeptides of the invention may also be used inthe production of lignocellulosic materials, such as pulp, paper andcardboard, from starch reinforced waste paper and cardboard, especiallywhere re-pulping occurs at pH above 7 and where amylases facilitate thedisintegration of the waste material through degradation of thereinforcing starch. The alpha-amylase of the invention is especiallyuseful in a process for producing a papermaking pulp from starch-coatedprinted-paper. The process may be performed as described in WO 95/14807,comprising the following steps:

a) disintegrating the paper to produce a pulp,b) treating with a starch-degrading enzyme before, during or after stepa), andc) separating ink particles from the pulp after steps a) and b).

The alpha-amylases of the invention may also be very useful in modifyingstarch where enzymatically modified starch is used in papermakingtogether with alkaline fillers such as calcium carbonate, kaolin andclays. With the alkaline alpha-amylases of the invention it becomespossible to modify the starch in the presence of the filler thusallowing for a simpler integrated process.

Desizing of Textiles, Fabrics and Garments

An alpha-amylase of the invention may also be very useful in textile,fabric or garment desizing. In the textile processing industry,alpha-amylases are traditionally used as auxiliaries in the desizingprocess to facilitate the removal of starch-containing size, which hasserved as a protective coating on weft yarns during weaving. Completeremoval of the size coating after weaving is important to ensure optimumresults in the subsequent processes, in which the fabric is scoured,bleached and dyed. Enzymatic starch breakdown is preferred because itdoes not involve any harmful effect on the fiber material. In order toreduce processing cost and increase mill throughput, the desizingprocessing is sometimes combined with the scouring and bleaching steps.In such cases, non-enzymatic auxiliaries such as alkali or oxidationagents are typically used to break down the starch, because traditionalalpha-amylases are not very compatible with high pH levels and bleachingagents. The non-enzymatic breakdown of the starch size does lead to somefiber damage because of the rather aggressive chemicals used.Accordingly, it would be desirable to use the alpha-amylases of theinvention as they have an improved performance in alkaline solutions.The alpha-amylases may be used alone or in combination with a cellulasewhen desizing cellulose-containing fabric or textile.

Desizing and bleaching processes are well known in the art. Forinstance, such processes are described in WO 95/21247, U.S. Pat. No.4,643,736, EP 119,920 hereby in corporate by reference.

Commercially available products for desizing include AQUAZYME® andAQUAZYME® ULTRA from Novozymes A/S.

Beer Making

The alpha-amylases of the invention may also be very useful in abeer-making process; the alpha-amylases will typically be added duringthe mashing process.

The invention is further described in the following paragraphs.1. An alpha-amylase variant of a parent alpha-amylase comprises amodification at one or more positions corresponding to position: 1, 7,9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90,98, 104, 109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134, 135,136, 139, 142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172, 173,174, 175, 176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206, 209,210, 212, T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269, 270,272, 273, 274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302, 303,304, 306, 310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339, 345,357, 365, 377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435, 439,444, 445, 458, 465, 466, 469, 473, 476 and 481, using SEQ ID NO: 1 fornumbering; and wherein each modification is independently asubstitution, insertion, or deletion, and wherein said variant has atleast 59%, e.g. at least 60%, e.g. at least 65%, e.g., at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, or at least 99%, but less than100% sequence identity to the polypeptide of SEQ ID NOs: 1-17, andwherein said variant has alpha-amylase activity.2. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 1,and wherein said variant has alpha-amylase activity.3. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 2,and wherein said variant has alpha-amylase activity.4. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3,and wherein said variant has alpha-amylase activity.5. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 4,and wherein said variant has alpha-amylase activity.6. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 5,and wherein said variant has alpha-amylase activity.7. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 6,and wherein said variant has alpha-amylase activity.8. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 7,and wherein said variant has alpha-amylase activity.9. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 8,and wherein said variant has alpha-amylase activity.10. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO: 9,and wherein said variant has alpha-amylase activity.11. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:10, and wherein said variant has alpha-amylase activity.12. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:11, and wherein said variant has alpha-amylase activity.13. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:12, and wherein said variant has alpha-amylase activity.14. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:13, and wherein said variant has alpha-amylase activity.15. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:14, and wherein said variant has alpha-amylase activity.16. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:15, and wherein said variant has alpha-amylase activity.17. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:16, and wherein said variant has alpha-amylase activity.18. The alpha-amylase variant of paragraph 1, wherein said variant of aparent alpha-amylase comprises a modification at one or more positionscorresponding to position: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56,58, 59, 60, 63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125,127, 130, 132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163,167, 169, 170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187,195, 202, 203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259,264, 265, 266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291,293, 295, 298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319,320, 323, 328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400,406, 408, 410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and481, using SEQ ID NO: 1 for numbering; and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59%, e.g. at least 60%, e.g. at least 65%, e.g., atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, or at least 99%,but less than 100% sequence identity to the polypeptide of SEQ ID NO:17, and wherein said variant has alpha-amylase activity.19. The alpha-amylase variant according to any one of the precedingparagraphs, wherein said variant of a parent alpha-amylase comprises amodification at one or more positions corresponding to position: 1, 7,9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84, 86, 90,98, 104, 109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134, 135,136, 139, 142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172, 173,174, 175, 176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206, 209,210, 212, T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269, 270,272, 273, 274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302, 303,304, 306, 310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339, 345,357, 365, 377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435, 439,444, 445, 458, 465, 466, 469, 473, 476 and 481, using SEQ ID NO: 1 fornumbering; and wherein each modification is independently asubstitution, insertion, or deletion, and wherein said variant has atleast 59%, e.g. at least 60%, e.g. at least 65%, e.g., at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, or at least 99%, but less than100% sequence identity to the polypeptide of SEQ ID NOs: 1-17, andwherein said variant has alpha-amylase activity and wherein said varianthas Improvement Factor (IF)>1.0 for a measure of wash performance whencompared to said parent polypeptide having alpha-amylase activity asshown in SEQ ID NO: 1 or SEQ ID NO: 2.20. The alpha-amylase variant according to any one of the precedingparagraphs, wherein said modifications at one or more positionscorresponding to positions: H1, G7, I9, Q11, N16, N19, N25, A37, W43,W48, N54, V56, Y58, G59, A60, L63, T81, E84, E86, R90, Q98, V104, G109,A111, F113, R116, Q118, Q125, R127, E130, S132, G133, T134, Y135, Q136,A139, G142, N144, G149, R158, Y160, D163, W167, Q169, S170, R171, Q172,L173, A174, N175, R176, Y178, R181, G182, A186, W187, N195, M202, Y203,A204, V206, D209, H210, E212, T227, L235, V238, M246, Q256, K259, V264,A265, E266, Y267, K269, N270, L272, G273, A274, L275, E276, W284, M286,V291, L293, Y295, Y298, Q299, N302, S303, S304, N306, R310, N311, N314,G315, L317, Q319, R320, S323, F328, G337, A339, Q345, D357, Q365, P377,D375, Q385, K391, Q395, R400, D406, W408, V410, S431, G435, W439, R444,Q445, R458, N465, Q466, W469, F473, G476 and V481, using SEQ ID NO: 1for numbering, and wherein each modification is independently asubstitution, insertion, or deletion, and wherein said variant has atleast 59%, e.g. at least 60%, e.g. at least 65%, e.g., at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, or at least 99%, but less than100% sequence identity to the polypeptide of SEQ ID NOs: 1-17, andwherein said variant has alpha-amylase activity and wherein said varianthas an Improvement Factor (IF) of >1.0 for a measure of wash performancewhen compared to said parent alpha-amylase as shown in SEQ ID NO: 1 orSEQ ID NO: 2.21. The variant according to any one of the preceding paragraphs,wherein the number of substitutions and/or deletion is 1 to 20, e.g., 1to 10 and 1 to 5, such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 substitutionsand/or deletions.22. The variant according to any one of the preceding paragraphs,wherein said variant comprises a deletion in two or more positionscorresponding to positions R181, G182, D183, and G184, using SEQ ID NO:1 for numbering.23. The variant according to paragraph 22, wherein said variantcomprises a deletion in the positions corresponding to the positionsR181+G182; R181+D183; R181+G184; G182+D183; G182+G184; or D183+G184,using SEQ ID NO: 1 for numbering.24. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance when compared to said parent alpha-amylase as shown inSEQ ID NO: 1 or SEQ ID NO: 2.25. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance in Model A detergent composition when compared to saidparent alpha-amylase as shown in SEQ ID NO: 1 or SEQ ID NO: 2.26. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance in ADW detergent composition with bleach when comparedto said parent alpha-amylase as shown in SEQ ID NO: 1 or SEQ ID NO: 2.27. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance in Model A detergent composition and/or ADW detergentcomposition with bleach when compared to said parent alpha-amylase asshown in SEQ ID NO: 1 or SEQ ID NO: 2.28. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance in ADW detergent composition with bleach assessed usingan Automatic Mechanical Stress Assay (AMSA) when compared to said parentalpha-amylase as shown in SEQ ID NO: 1 or SEQ ID NO: 2.29. The variant according to any one of the preceding paragraphs,wherein said variant has an Improvement Factor (IF) of at least 1.1, atleast 1.2, at least 1.3, at least 1.4, at least 1.5, at least 1.6, atleast 1.7, at least 1.8, at least 1.9, at least 2.0, at least 2.2, atleast 2.4, at least 2.6, at least 2.8, at least 3.0, at least 3.2, atleast 3.4, at least 3.6, at least 3.8, or at least 4.0 for a measure ofwash performance in ADW detergent composition with bleach assessed usingan a full scale automatic dishwash (ADW) when compared to said parenthaving alpha-amylase activity as shown in SEQ ID NO: 1 or SEQ ID NO: 2.30. The alpha-amylase variant according to preceding paragraphs, whereinthe parent polypeptide has at least 59%, e.g. at least 60%, e.g., atleast 62%, at least 63%, at least 65%, at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99% or 100% sequence identity to thepolypeptide of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4,SEQID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14,SEQ ID NO: 15, SEQ ID NO: 16 or SEQ ID NO: 17.31. The alpha-amylase variant according to paragraph 11, wherein theparent polypeptide comprises or consists of the polypeptide of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ IDNO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ IDNO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQID NO: 16 or SEQ ID NO: 17.32. The alpha-amylase variant according to any one of the precedingparagraphs, which has at least 60%, e.g., at least 65%, at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%identity, at least 96%, at least 97%, at least 98%, or at least 99%, butless than 100%, sequence identity to the amino acid sequence of theparent polypeptide.33. The alpha-amylase variant according to any one of the precedingparagraphs, wherein said variant comprises a) a deletion and/or asubstitution at two or more positions corresponding to positions R181,G182, D183, and G184, using SEQ ID NO: 1 for numbering and b) amodification at one or more positions corresponding to positions H1, G7,I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63,T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127,E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160,D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181,G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227,L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272,G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302,S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328,G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406,W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473,G476 and V481, using SEQ ID NO: 1 for numbering.34. The alpha-amylase variant according to any one of the precedingparagraphs, wherein said variant comprises one or more of the followingmodifications: H1*; G7A; I9M; Q11H; N16E; N16H; N16Y; N19D; N25K; N25M;N25T; A37V; W43Y; W48Y; N54Q; N54S; V56I; V56T; Y58F; G59A; A60S; A60T;L63F; T81A; E84Q; E86L; R90H; R90N; R90Q; Q98N; V104A; G109A; A111T;F113A; F113G; F113N; F113Q; F113T; F113Y; R116C; R116D; R116F; R116H;R116K; R116N; R116S; R116Y; Q118N; Q118T; Q125G; Q125S; R127A; R127F;R127H; R127L; R127N; R127T; E130D; E130F; E130I; E130K; E130L; E130N;E130S; E130T; E130V; S132A; S132D; S132F; S132L; S132M; S132P; S132T;G133D; T134C; T134E; T134N; T134P; T134R; T134S; T134W; T134Y; T134A;Y135H; Q136D; Q136E; Q136G; Q136N; Q136R; Q136S; A139T; G142H; N144H;G149A; R158C; R158H; R158K; R158Q; R158S; Y160D; Y160F; T160H; Y160N;D163H; W167*; Q169*; Q169E; Q169F; S170*; 5170A; R171*; R171H; R171K;R171L; R171Y; Q172*; Q172K; Q172N; Q172S; L173*; L173K; A174*; A174N;A174S; N175*; N175S; R176*; R176A; Y178F; R181*; R181A; R181C; R181E;R181G; R181H; R181K; R181N; R181Q; G182*; G182T; A186D; A186E; A186H;A186K; A186N; A186T; W187M; N195D; N195F; N195H; N195Q; N195Y; M202L;Y203L; A204V; V206L; D209N; H210K; H210N; E212D; T227K; T227N; L235I;V238A; M246I; M246L; M246T; M246V; Q256H; Q256N; K259G; K259H; V264A;V246I; V264T; A265G; E266V; Y267F; Y267H; Y267L; K269M; K269Q; K269R;N270G; N270P; L272I; G273V; A274K; A274S; L275A; L275I; L275V; E276N;W284R; W284Y; M286L; V291A; V291I; V291T; L293I; L293V; Y295F; Y298E;Y298N; Q299N; Q299Y; N302A; N302H; N302K; N302Q; 5303G; 5304G; 5304Q;N306H; N306K; N306Q; N306R; N306Y; R310N; R310S; R310Q; N311K; N311R;N314Q; G315N; L317V; Q319H; R320K; R320Q; R320S; S323T; H323K; F328L;G337D; G337E; A339S; Q345N; Q345R; L357F; Q365A; Q365C; Q365E; Q365H;Q365K; Q365M; Q365S; P377K; P377T; D375H; D375N; D375Y; Q386L; K391A;Q395K; R400S; D406H; W408H; V410I; S431F; G435C; W439R; W439T; R444T;Q445S; R458K; N465G; Q466S; W469N; W469Y; F473I; F473P; F473S; G476K;and V481A, using SEQ ID NO: 1 for numbering.34. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

A60T, Y135H, Q169E, W48Y, A174*, M246L, R90Q, Y203L+V206L, Q169F+S170A,S132D+G133D, N195F+Q365C, N195F+Q365S, N195F+Q365M, R127L+Q365S,R127N+Q365S, R127L+Q365C, N195F+Q365K, I9M+N195F, R171H+Q365C,Y267F+Q365S, F328L+Q365S, R171L+Q365S, N195F+M286L, T134P+N195F,S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,M246L+L275V+Q365S, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C, I9M+N195F+Q365S,V264I+Y267F+Q365K, R127L+M246V+Q365S, A60S+R171Y+Q365S,R127N+M246T+L275V, V104A+N195F+Q365S, V264I+F328L+Q365S,R171H+N302H+Q365S, V264I+Y267F+Q365S, A174*+N175*+Y178F,V264I+A265G+Q365C, R171L+N302H+Q365C, R171L+M246V+Q365C,R171Y+M246L+Q365S, V264T+Y267F+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G4350, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, R127N+M246L+L275V+Q365S,R171H+M246L+L275V+Q365S, R127H+M246I+Q365C+V481A,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, W187M+N195F+H210K+Q365S,R127H+M246T+L275A+Q365S, I9M+A111T+N195F+Q365S, I9M+Y58F+N195F+Q365S,V264T+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F+Q365C, V264I+Y267F+V291A+F328L+Q365S,R127N+R171H+M246L+L275V+Q365S, V264I+A265G+V291A+F328L+Q365S,R127N+M246I+A265G+Y267H+Q365S, E130I+T134A+R181N+N195F+Q365S,R127N+M246L+L275V+N306R+Q365S, I9M+N195F+V206L+D209N+Q365S,R116S+S132F+R181N+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,A37V+V264I+Y267F+F328L+Q365C, Q11H+V264I+Y267F+F328L+Q365C,V264I+Y267F+V291T+F328L+Q365S, Q169*+R171*+Q172*+L173*+A174*,R116Y+S132P+Q136N+N195F+Q365S, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+Y267F+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,E130D+T134Y+R181H+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N195F+G337D+Q365S+P375K+D377N, R127N+R171L+M246L+L275V+Q365S,N195F+G337E+Q365S+P375K+D377N, Q11H+V264I+A265G+Y267F+Q365C,R158Q+Y160N+W187M+N195F+Q365S, V264I+Y267F+Q365K+W408H+V410I,R127N+R171Y+M246L+L275V+Q365S, N54Q+F113T+R171H+N302A+Q365C,V264A+Y267F+V291T+F328L+Q365C, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R116C+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,V264I+A265G+Y267F+V291I+F328L+Q365S, N16E+N195F+G337D+Q365S+P375K+D377N,V264I+A265G+Y267F+F328L+Q365S+Q395K,E130N+T134C+R181Q+A186N+N195F+Q365S,V264I+A265G+Y267F+V291A+F328L+Q365C,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302 K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S, A186D+N195F,A60S+R171Y+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130D+T134Y+R181H+N195F+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S, E130I+T134A+R181N+N195F+Q365S,E130I+T134A+R181N+N195F+Q365S, E130L+T134N+R181W+A186T+N195F+Q365S,E130N+T134C+R181Q+A186N+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S, E86L+R171L+N306R+Q365S,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,E86L+R171L+N306R+Q365S, F113Q+R116H, F113T+R171K+G273V, F328L+Q365S,G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,

H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q125G+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K269M+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A, H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A, H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+G184K+N195F+Q365S,I9M+N195F+K269Q+A274K+Q365S, I9M+N195F+N270G+Q365S,I9M+N195F+N270P+Q365S, I9M+N195F+Q365C, I9M+N195F+V206L+D209N+Q365S,I9M+N195F+V291A+Q365S, I9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S,I9M+G184K+N195F+Q365S,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,

I9M+N195F+Q365C,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,I9M+R127N+N195F+Q365S, I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+N195F+Q365S, M202L, M246L+L275V+Q365S+D406H, M246L,M246L+L275V+Q365S, M246L+L275V+Q365S+D406H,N16E+N195F+G337D+Q365S+P375K+D377N, N16E+N195F+G337E+Q365S+P375T+D377H,N16E+N195F+G337E+Q365S+P375T+D377H,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S, N195F+G337D+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+Q365S, N54Q+F113T+R171H+N302A+Q365C,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Q11H+V264I+A265G+Q365C, Q11H+V264I+A265G+Y267F+Q365C,Q11H+V264I+Y267F+F328L+Q365C, Q11H+Y267F+Q365C, Q11H+V264I+A265G+Q365C,Q169*+S170*+A174*, Q169*+S170*+R171*+A174*,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,R116K+S132L+Q136R+R181C+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116S+S132F+R181N+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S, R116Y+S132P+Q136N+N195F+Q365S,R127H+L235I+Q365S+G435C, R127L+M246V+Q365S, R127L+Q365C, R127L+Q365S,R127L, R127N+M246L+L275V+Q365S, R127N+Q365S,R127N+R171L+M246L+L275V+Q365S, R127N+R171H+M246L+L275V+Q365S,R171H+Q365C, R171H+M246L+L275V+Q365S, R171H+N302H+Q365S, R171H,R171H+Q365C, R171L+N302H+Q365C, R171L+Q365S, R171Y++M246L+Q365S,R171Y+N306K, R181S+G184S, V104A+N195F+Q365S,V264A+Y267F+V291T+F328L+Q365C, V264I+A265G+V291A+F328L+Q365S,V264I+A265G+V291A+Q365C, V264I+A265G+Y267F+F328L+Q365S+Q395K,V264I+A265G+Y267F+F328L+Q365S, V264I+A265G+Y267F+V291I+F328L+Q365S,V264I+A265G+Y267F, V264I+F328L+Q365C, V264I+F328L+Q365S, V264I+L357F,V264I+Q365C, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+Y267F+F328L+Q365C, V264I+Y267F+F328L+Q365K,V264I+Y267F+F328L+Q365S, V264I+Y267F+Q365K+W408H+V410I,V264I+Y267F+Q365K, V264I+Y267F+Q365S, V264I+Y267F+V291A+F328L+Q365C,V264I+Y267F+V291A+F328L+Q365S, V264I+Y267F+V291T+F328L+Q365S,V264I+A265G+Y267F, V264I+F328L+Q365C, V264I+Y267F+F328L+Q365K,V264I+Y267F+V291T+F328L+Q365S, V264T+A265G+Y267F+V291A+F328L+Q365C,V264T+A265G+Y267L+F328L+Q365C, V264T+V291A+F328L+Q365S,V264T+Y267F+Q365S, V264T+Y267L+F328L+Q365C,V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6K,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,W187M+N195F+H210K+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K, Y160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Y+E212 D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y267F+Q365S, Y267F+V291A+F328L+Q365C, Y267F+V291A+F328L+Q365S,Y267F+Q365S, Y267F+V291A+F328L+Q365S,

Y295F, using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.1 in Model A detergentcomposition as measure of wash performance and/or ADW detergentcomposition with bleach.35. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

A60T, Y135H, Q169E, W48Y, A174*, M246L, R90Q, Y203L+V206L, Q169F+S170A,S132D+G133D, N195F+Q365C, N195F+Q365S, N195F+Q365M, N195F+Q365K,R127L+Q365S, R127N+Q365S, R127L+Q365C, R171L+Q365S, R171H+Q365C,Y267F+Q365S, F328L+Q365S, I9M+N195F, N195F+M286L, T134P+N195F,S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,M246L+L275V+Q365S, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C, I9M+N195F+Q365S,V264I+Y267F+Q365K, R127L+M246V+Q365S, A60S+R171Y+Q365S,R127N+M246T+L275V, V104A+N195F+Q365S, V264I+F328L+Q365S,R171H+N302H+Q365S, V264I+Y267F+Q365S, A174*+N175*+Y178F,V264I+A265G+Q365C, R171L+N302H+Q365C, R171L+M246V+Q365C,R171Y+M246L+Q365S, V264T+Y267F+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G4350, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, I9M+R127N+N195F+Q365S,R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, I9M+R171H+N195F+Q365S, W187M+N195F+H210K+Q365S,R127H+M246T+L275A+Q365S, I9M+A111T+N195F+Q365S, I9M+Y58F+N195F+Q365S,V264T+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F+Q365C, V264I+Y267F+V291A+F328L+Q365S,R127N+R171H+M246L+L275V+Q365S, V264I+A265G+V291A+F328L+Q365S,R127N+M246I+A265G+Y267H+Q365S, E130I+T134A+R181N+N195F+Q365S,R127N+M246L+L275V+N306R+Q365S, I9M+N195F+V206L+D209N+Q365S,R116S+S132F+R181N+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,A37V+V264I+Y267F+F328L+Q365C, Q11H+V264I+Y267F+F328L+Q365C,V264I+Y267F+V291T+F328L+Q365S, Q169*+R171*+Q172*+L173*+A174*,R116Y+S132P+Q136N+N195F+Q365S, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+Y267F+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,E130D+T134Y+R181H+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N195F+G337D+Q365S+P375K+D377N, R127N+R171L+M246L+L275V+Q365S,N195F+G337E+Q365S+P375K+D377N, Q11H+V264I+A265G+Y267F+Q365C,R158Q+Y160N+W187M+N195F+Q365S, V264I+Y267F+Q365K+W408H+V410I,R127N+R171Y+M246L+L275V+Q365S, N54Q+F113T+R171H+N302A+Q365C,V264A+Y267F+V291T+F328L+Q365C, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R116C+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,V264I+A265G+Y267F+V291I+F328L+Q365S, N16E+N195F+G337D+Q365S+P375K+D377N,V264I+A265G+Y267F+F328L+Q365S+Q395K,E130N+T1340+R181Q+A186N+N195F+Q365S,V264I+A265G+Y267F+V291A+F328L+Q365C,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302 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Y160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A,Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A,Y160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A,H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6K,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A,H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R44 4T+Q445S+Q466S, usingSEQ ID NO: 1 for numbering and wherein said variant has an improvedrelative specific activity of at least ≥1.1 in Model A detergentcomposition as a measure of wash performance compared to parentalpha-amylase of SEQ ID NO: 2.36. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

M246L, N195F+Q365C, N195F+Q365S, N195F+Q365M, N195F+Q365K, R127L+Q365S,R127N+Q365S, R127L+Q365C, R171H+Q365C, Y267F+Q365S, F328L+Q365S,I9M+N195F, S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,M246L+L275V+Q365S, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C, I9M+N195F+Q365S,V264I+Y267F+Q365K, R127L+M246V+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, I9M+R127N+N195F+Q365S,R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, V264I+Y267F+V291A+F328L+Q365S,V264I+A265G+V291A+F328L+Q365S, R127N+M246I+A265G+Y267H+Q365S,E130I+T134A+R181N+N195F+Q365S, R127N+M246L+L275V+N306R+Q365S,I9M+N195F+V206L+D209N+Q365S, R116S+S132F+R181N+N195F+Q365S,E130T+T134Y+A186K+N195F+Q365S, A37V+V264I+Y267F+F328L+Q365C,Q11H+V264I+Y267F+F328L+Q365C, V264I+Y267F+V291T+F328L+Q365S,Q169*+R171*+Q172*+L173*+A174*, R116Y+S132P+Q136N+N195F+Q365S,N195F+G337D+Q365S+P375K+D377Y, V264I+A265G+Y267F+F328L+Q365S,I9M+N195F+K269Q+A274K+Q365S, E130D+T134Y+R181H+N195F+Q365S,R116N+S132M+Q136R+N195F+Q365S, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R1160+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A, using SEQ ID NO: 1 for numbering and wherein said variant has animproved relative specific activity of at least ≥1.5 in Model Adetergent composition as a measure of wash performance compared toparent alpha-amylase of SEQ ID NO: 2.37. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

N195F+Q365C, N195F+Q365S, N195F+Q365M, R127L+Q365S, R127N+Q365S,S170*+R171*+A174*, V264I+A265G+Y267F, Q169*+S170*+A174*,V264I+V291I+Q365S, V264I+V291T+Q365K, V264I+F328L+Q365C,I9M+Y160H+N195F+Q365S, I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C,I9M+R171H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S,Q169*+S170*+R171*+A174*, M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S,I9M+R127N+N195F+Q365S, R127N+M246L+L275V+Q365S, R171H+M246L+L275V+Q365S,R127H+M246I+Q365C+V481A, R127N+M246L+L275V+Q365S,Y267F+V291A+F328L+Q365C, V264I+Y267F+V291A+F328L+Q365S,V264I+A265G+V291A+F328L+Q365S, R127N+M246I+A265G+Y267H+Q365S,E130I+T134A+R181N+N195F+Q365S, R127N+M246L+L275V+N306R+Q365S,I9M+N195F+V206L+D209N+Q365S, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,

H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has animproved relative specific activity of at least ≥2.0 in Model Adetergent composition as a measure of wash performance compared toparent alpha-amylase of SEQ ID NO: 2.38. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

N195F+Q365C, S170*+R171*+A174*, I9M+Y160H+N195F+Q365S,R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, Q169*+5170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,

Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an improvedrelative specific activity of at least ≥2.5 in Model A detergentcomposition as a measure of wash performance compared to parentalpha-amylase of SEQ ID NO: 2.39. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

Y295F, M246L, M202L, R181S+G184S, F113Q+R116H, V264I+L357F, Y267F+Q365S,R171H, R171Y+N306K, R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S,R171L+N302H+Q365C, R171Y++M246L+Q365S, F328L+Q365S, R127L, R127N+Q365S,R127L+Q365C, R127L+Q365S, R127L+M246V+Q365S, A186D+N195F, V264I+Q365C,I9M+N195F+Q365C, V264I+A265G+Y267F, V264I+V291T+Q365K, A60S+R171Y+Q365S,V264I+Y267F+Q365K, V264I+F328L+Q365C, M246L+L275V+Q365S,V264T+Y267F+Q365S, V264I+F328L+Q365S, Q11H+Y267F+Q365C,V104A+N195F+Q365S, V264I+V291I+Q365S, V264I+Y267F+Q365S,F113T+R171K+G273V, Q169*+S170*+A174*, I9M+R171H+N195F+Q365S,I9M+R127N+N195F+Q365S, E86L+R171L+N306R+Q365S, I9M+A186E+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S, R171H+M246L+L275V+Q365S,I9M+N195F+V291A+Q365S, I9M+G184K+N195F+Q365S, M246L+L275V+Q365S+D406H,Y267F+V291A+F328L+Q365S, Y267F+V291A+F328L+Q365C,V264I+Y267F+F328L+Q365K, I9M+R171L+N195F+Q365S, Q11H+V264I+A265G+Q365C,V264T+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365S,R127N+M246L+L275V+Q365S, I9M+N195F+N270G+Q365S, I9M+N195F+N270P+Q365S,V264T+Y267L+F328L+Q365C, V264I+A265G+V291A+Q365C,W187M+N195F+H210K+Q365S, V264I+Y267F+F328L+Q365C,Q169*+S170*+R171*+A174*, 19M+N195F+V206L+D209N+Q365S,N195F+G337D+Q365S+P375K+D377N, V264I+Y267F+V291T+F328L+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+V291A+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,V264I+Y267F+Q365K+W408H+V410I, E130D+T134Y+R181H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, E130I+T134A+R181N+N195F+Q365S,R116Y+S132P+Q136N+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,V264I+A265G+Y267F+F328L+Q365S, Q11H+V264I+Y267F+F328L+Q365C,Q11H+V264I+A265G+Y267F+Q365C, E130T+T134Y+A186K+N195F+Q365S,R116S+S132F+R181N+N195F+Q365S, V264A+Y267F+V291T+F328L+Q365C,N54Q+F113T+R171H+N302A+Q365C, R127N+R171L+M246L+L275V+Q365S,V264T+A265G+Y267L+F328L+Q365C, V264I+Y267F+V291A+F328L+Q365C,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130V+T134N+R181Y+A186K+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,V264I+A265G+Y267F+F328L+Q365S+Q395K, N16E+N195F+G337D+Q365S+P375K+D377N,E130N+T134C+R181Q+A186N+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116K+S132L+Q136R+R181C+N195F+Q365S,V264I+A265G+Y267F+V291I+F328L+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391A,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.1 in ADW detergent compositionwith bleach assessed using AMSA.40. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

Y295F, M246L, M202L, R181S+G184S, F113Q+R116H, Y267F+Q365S, R171H,R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S, R171L+N302H+Q365C,R171Y++M246L+Q365S, F328L+Q365S, I9M+N195F+Q365C, V264I+A265G+Y267F,V264I+V291T+Q365K, A60S+R171Y+Q365S, V264I+Y267F+Q365K,V264I+F328L+Q365C, M246L+L275V+Q365S, V264T+Y267F+Q365S,V264I+F328L+Q365S, Q11H+Y267F+Q365C, I9M+R171H+N195F+Q365S,I9M+R127N+N195F+Q365S, E86L+R171L+N306R+Q365S, I9M+A186E+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+N195F+V291A+Q365S,I9M+G184K+N195F+Q365S, M246L+L275V+Q365S+D406H, Y267F+V291A+F328L+Q365S,Y267F+V291A+F328L+Q365C, V264I+Y267F+F328L+Q365K, I9M+R171L+N195F+Q365S,Q11H+V264I+A265G+Q365C, I9M+N195F+V206L+D209N+Q365S,N195F+G337D+Q365S+P375K+D377N, V264I+Y267F+V291T+F328L+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+V291A+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,V264I+Y267F+Q365K+W408H+V410I, E130D+T134Y+R181H+N195F+Q365S,V264I+Y267F+V291A+F328L+Q365S, E130I+T134A+R181N+N195F+Q365S,R116Y+S132P+Q136N+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.5 in ADW detergent compositionwith bleach assessed using AMSA.41. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

M202L, R171H+Q365C, R171L+Q365S, R171H+N302H+Q365S, R171L+N302H+Q365C,I9M+N195F+Q365C, V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,I9M+A186E+N195F+Q365S, I9M+Y160H+N195F+Q365S, I9M+A186H+N195F+Q365S,I9M+N195F+V206L+D209N+Q365S, N195F+G337D+Q365S+P375K+D377N,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥2.0 in ADW detergent compositionwith bleach assessed using AMSA.42. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

M202L, R171H+Q365C, I9M+N195F+Q365C,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,

Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥2.5 in ADW detergent composition with bleachassessed using AMSA.43. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, usingSEQ ID NO: 1 for numbering and wherein said variant has an ImprovementFactor (IF) of at least ≥2.5 in ADW detergent composition with bleachassessed using AMSA.44. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

V264I+A265G+Y267F, R171L+Q365S, Y267F+Q365S, R127L+Q365S,V264I+F328L+Q365C, M246L+L275V+Q365S+D406H, Q11H+V264I+A265G+Q365C,R127N+R171H+M246L+L275V+Q365S, V264I+Y267F+V291T+F328L+Q365S,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,R127H+L235I+Q365S+G435C, N195F+Q365S, I9M+N195F+Q365C,I9M+R127N+N195F+Q365S, I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S,I9M+R171H+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+G184K+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N, N195F+G337D+Q365S+P375K+D377Y,E130I+T134A+R181N+N195F+Q365S, R116N+S132A+Q136D+R181K+N195F+Q365S,N16E+N195F+G337E+Q365S+P375T+D377H, E130D+T134W+R181K+A186E+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391AH1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.1 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.45. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

V264I+A265G+Y267F, R127N+R171H+M246L+L275V+Q365S,V264I+Y267F+V291T+F328L+Q365S, I9M+N195F+Q365C, I9M+R127N+N195F+Q365S,I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N, N16E+N195F+G337E+Q365S+P375T+D377H,E130D+T134W+R181K+A186E+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.46. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥2.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.47. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202 L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥2.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.48. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206LK+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+R320S+K391A,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202 L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.48. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391Ausing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 40°C.49. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

Y295F, M246L, N195F+Q365S, R171H+Q365C, V264I+A265G+Y267F,E86L+R171L+N306R+Q365S, V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.1 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.50. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,N195F+Q365S, I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,N195F+G337E+Q365S+P375K+D377N,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.2 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.51. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

N195F+Q365S, V264I+A265G+Y267F, E86L+R171L+N306R+Q365S,V264I+Y267F+V291T+F328L+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,I9M+R127N+N195F+Q365S, I9M+R171L+N195F+Q365S,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.3 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.52. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:V264I+A265G+Y267F

V264I+Y267F+V291T+F328L+Q365S I9M+R171L+N195F+Q365SH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391AH1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458Kusing SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥1.5 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.53. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥2.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.54. The variant according to any one of the preceding paragraphs,wherein said variant is selected from the group consisting of:

H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,

H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,using SEQ ID NO: 1 for numbering and wherein said variant has anImprovement Factor (IF) of at least ≥3.0 in ADW detergent compositionwith bleach assessed using a full scale automatic dishwash (ADW) at 45°C.55. A polynucleotide encoding said variant according to any one ofparagraphs 1 to 54.56. A nucleic acid construct comprising said polynucleotide according toparagraph 55.57. An expression vector comprising said polynucleotide according toparagraph 55.58. A host cell comprising said polynucleotide according to paragraph55, said nucleic acid construct according to paragraph 56, or saidexpression vector according to paragraph 57.59. A method of producing an alpha-amylase variant, comprising:cultivating said host cell according to paragraph 58 under conditionssuitable for expression of said variant; and (b) recovering saidvariant.60. A composition comprising the variant according to any one ofparagraphs 1 to 54 and at least one additional active component.61. The composition according to paragraph 60, wherein one or moreadditional active component is selected from the group consisting of oneor more enzymes, oxidizing agents, bleach activators, bleach catalysts,chelating agents, bulking agents, builders, buffering agents,structurants, sequestrants, optical brighteners, antifoaming agents,enzymes, fragrances, anti-redeposition agents, crystal growthinhibitors, metal care agents, glass care agents, skin conditioningagents, softness extenders, emulsifiers, and colorants.62. The composition according to paragraphs 60-61, wherein theadditional enzyme is selected from the group consisting of proteases,amylases, phospho-lipases, esterases, lipases, cutinases, cellulases,endoglucanases, xyloglucanases, xylanases, pectinases, hemicellulasespectin lyases, xanthanases, peroxidases, keratinases haloperoxygenases,catalases, mannanases, lechinase, RNase, DNAse, reductases, oxidases,phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases,pento-sanases, malanases, β-glucanases, arabinosidases, hyaluronidase,chondroitinase, and laccase or any mixture thereof.63. The composition according to paragraphs 60-62, further comprising asurfactant, wherein the surfactant is one or more surfactants selectedfrom the group consisting of an anionic surfactant, a cationicsurfactant, a non-ionic surfactant, zwitterionic surfactant, andamphoteric surfactants or any mixtures thereof.64. The composition according to any one of paragraphs 60-63, whereinsaid composition is a detergent composition.65. The composition according to paragraphs 60-64, wherein the detergentcomposition is a liquid laundry detergent composition, a powder laundrydetergent composition, a liquid dishwash detergent composition, or apowder dishwash detergent composition.66. The composition according to paragraphs 60-65, wherein saidcomposition is a liquid or powder laundry detergent composition.67. The composition according to paragraphs 60-66, wherein saidcomposition is a liquid or powder automatic dishwashing (ADW) detergentcomposition.68. The composition according to any one of paragraphs 60-67, whereinsaid composition is a liquid manual dishwashing detergent composition.69. Use of a variant according to any one of paragraphs 1 to 54, or of acomposition according to any one of paragraphs 60-68, in a domestic orindustrial cleaning process.70. Use according to paragraph 69 for cleaning of fabric, for examplelaundry.71. Use according to paragraph 70 for hard surface cleaning, cleaning ofceramic, metal, plastic or glass material, for example dishwashing.72. A method for removal of soils from fabric or hard surfacescomprising contacting the fabric or hard surfaces contaminated withsoils with a composition according to any one of paragraphs 60-68.73. A method according to paragraph 72 for cleaning of fabric, forexample laundry.74. A method according to paragraph 72 for hard surface cleaning, forexample dishwashing.75. A method according to paragraph 74 wherein the method is performedusing an automated dishwasher.76. A method of dishwashing in an automatic dishwashing machine using acomposition according to any one of paragraphs 60-68 comprising thesteps of adding said detergent composition in a detergent compositioncompartment in said automatic dishwashing machine, and releasing saiddetergent composition during a main-wash cycle.77. A method of laundering in an automatic laundering machine using acomposition according to any one of paragraphs 60-68, comprising thesteps of adding said detergent composition in a detergent compositioncompartment in said automatic laundering machine, and releasing saiddetergent composition during a main wash cycle.78. A method of improving the performance, such as wash performance, ofa parent alpha-amylase having the amino acid sequence of SEQ ID NO: 2,or having at least 59% sequence identity hereto, said method comprisingthe steps of a) introducing a substitution and/or deletion at two ormore positions corresponding to positions R181, G182, D183, and G184,using SEQ ID NO: 1 for numbering; and b) introducing a deletion and/orsubstitution at one or more positions selected from the group consistingof: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84,86, 90, 98, 104, 109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134,135, 136, 139, 142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172,173, 174, 175, 176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206,209, 210, 212, T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269,270, 272, 273, 274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302,303, 304, 306, 310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339,345, 357, 365, 377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435,439, 444, 445, 458, 465, 466, 469, 473, 476 and/or 481, using SEQ ID NO:1; said method thereby providing an alpha-amylase variant of said parentalpha-amylase, wherein said variant has at least 59%, such as at least60%, such as at least 65%, such as at least 70%, such as at least 75%,such as at least 80%, such as at least 85%, such as at least 90%, suchas at least 95%, such as at least 97%, such as at least 99%, but lessthan 100%, sequence identity to the amino acid sequence as set forth inSEQ ID NOs: 1-17, and wherein said variant has alpha-amylase activityand improved performance as compared to said parent alpha-amylase havingalpha-amylase activity as shown in SEQ ID NO: 2.

The present invention is further described by the following examplesthat should not be construed as limiting the scope of the invention.

Materials and Method Strains

The DNA encoding the GH13_5 alpha-amylase was isolated from Bacillus spisolated from soil samples collected in Denmark and sequenced asdescribed in WO 2000/060058.Chemicals used as buffers and substrates were commercial products of atleast reagent grade.CS-28 swatch-DM-277 (double soiled Mix starch) was purchased from Centerfor Testmaterials BV, Stoomloggerweg 11, 3133 KT Vlaardingen, theNetherlands.

Assays for Alpha-Amylase Activity

1. PNP-G7 Assay

The alpha-amylase activity was determined by employing the pNP-G7substrate (PNP-G7 the abbreviation for4,6-ethylidene(G7)-p-nitrophenyl(G1)-α,D-maltoheptaoside, a blockedoligosaccharide which is cleaved by an endo-amylase, such as analpha-amylase).

An antibody was diluted in Phosphate buffered saline (PBS) (0.010 MPhosphate buffer pH7.4, 0.0027M KCl, 0.14M NaCl) buffer to concentrationof 10 μg/ml. A maxisorp microtiter plate was coated with antibody byadding 100 μl diluted antibody (10 μg/ml) to each well and incubated for1 h at room temperature (RT) and mixing at 800 rpm. After incubation themicrotiter plate was washed (using Bio-Tek ELx405 ELISA washer) with3×200 μl Phosphate buffered saline with 0.05% Tween (PBST) (0.010 MPhosphate buffer pH7.4, 0.0027M KCl, 0.14M NaCl, 0.05% Tween 20) buffer.

Microtiter plates with the alpha-amylase variants culture broths werespun down and supernatants transferred to new microtiter plates anddiluted 4× in PBST buffer. 100 μl diluted supernatant was transferred toantibody coated maxisorp microtiter plate and incubated for 1 h at RTand mixing at 800 rpm. After incubation microtiter plates were washed inPBST buffer (3×200 μl, ELISA washer).

Upon the cleavage of the pNP-G7 substrate, the alpha-Glucosidaseincluded in the kit used is digested and the hydrolysed substrateliberates a free pNP molecule which has a yellow color and thus can bemeasured by visible spectophometry at Abs=405 nm (400-420 nm.). Kitscontaining pNP-G7 substrate and alpha-Glucosidase are manufactured byRoche/Hitachi (cat. No. 11876473). 100 μl pNP-G7 substrate was added toall wells and mixed for 1 minute before measuring absorbance at 405 nm.The slope (absorbance per minute) is determined and only the linearrange of curve is used.

The slope of the time dependent absorption-curve is directlyproportional to the activity of the alpha-amylase in question under thegiven set of conditions.

The specific alpha-amylase activity may also be determined by otheractivity assays, such as amylazyme activity assay, Phadebas activityassay, or reducing sugar activity assay as described below.

2. Amylazyme Activity Assay

Amylazyme activity assay (from Megazyme, Ireland): An Amylazyme tabletincludes interlinked amylose polymers that are in the form of globularmicrospheres that are insoluble in water. A blue dye is covalently boundto these microspheres. The interlinked amylose polymers in themicrosphere are degraded at a speed that is proportional to thealpha-amylase activity. When the alpha-amylase degrades the amylosepolymers, the released blue dye is water soluble and concentration ofdye can be determined by measuring absorbance at 650 nm. Theconcentration of blue is proportional to the alpha-amylase activity inthe sample.

The amylase sample to be analysed is diluted in activity buffer with thedesired pH. One substrate tablet is suspended in 5 mL activity bufferand mixed on magnetic stirrer. During mixing of substrate transfer 150μl to microtiter plate (MTP). Add 30 μl diluted amylase sample to 150 μlsubstrate and mix. Incubate for 15 minutes at 37° C. The reaction isstopped by adding 30μl 1M NaOH and mix. Centrifuge MTP for 5 minutes at4000×g. Transfer 100 μl to new MTP and measure absorbance at 620 nm.

The amylase sample should be diluted so that the absorbance at 650 nm isbetween 0 and 2.2, and is within the linear range of the activity assay.

3. Phadebas Assay

A Phadebas tablet (from for example Magle Life Sciences, Lund, Sweden)includes interlinked starch polymers that are in the form of globularmicrospheres that are insoluble in water. A blue dye is covalently boundto these microspheres. The interlinked starch polymers in themicrosphere are degraded at a speed that is proportional to thealpha-amylase activity. When the alpha-amylase degrades the starchpolymers, the released blue dye is water soluble and concentration ofdye can be determined by measuring absorbance at 650 nm. Theconcentration of blue is proportional to the alpha-amylase activity inthe sample.

The amylase sample to be analysed is diluted in activity buffer with thedesired pH. One substrate tablet is suspended in 5 mL activity bufferand mixed on magnetic stirrer. During mixing of substrate transfer 150μl to microtiter plate (MTP). Add 30 μl diluted amylase sample to 150 μlsubstrate and mix. Incubate for 15 minutes at 37° C. The reaction isstopped by adding 30 μl 1M NaOH and mix. Centrifuge MTP for 5 minutes at4000×g. Transfer 100 μl to new MTP and measure absorbance at 620 nm.

The measured absorbance is directly proportional to the specificactivity (activity/mg of pure alpha-amylase protein) of thealpha-amylase in question under the given set of conditions.

4. Reducing Sugar Activity Assay

Number of reducing ends formed by the alpha-amylase hydrolysing thealpha-1,4-glycosidic linkages in starch is determined by reaction withp-Hydroxybenzoic acid hydrazide (PHBAH). After reaction with PHBAH thenumber of reducing ends can be measured by absorbance at 405 nm and theconcentration of reducing ends is proportional to the alpha-amylaseactivity in the sample.

The corns starch substrate (3 mg/ml) is solubilised by cooking for 5minutes in milliQ water and cooled down before assay. For the stopsolution prepare a Ka-Na-tartrate/NaOH solution (K-Na-tartrate (Merck8087) 50 g/l, NaOH 20 g/l) and prepare freshly the stop solution byadding p-Hydroxybenzoic acid hydrazide (PHBAH, Sigma H9882) toKa-Na-tartrate/NaOH solution to 15 mg/ml.

In PCR-MTP 50 μl activity buffer is mixed with 50 μl substrate. Add 50μl diluted enzyme and mix. Incubate at the desired temperature in PCRmachine for 5 minutes. Reaction is stopped by adding 75 μl stop solution(Ka-Na-tartrate/NaOH/PHBAH). Incubate in PCR machine for 10 minutes at95° C. Transfer 150 μl to new MTP and measure absorbance at 405 nm.

The measured absorbance is directly proportional to the specificactivity (activity/mg of pure alpha-amylase protein) of thealpha-amylase in question under the given set of conditions.

EXAMPLES Example 1: Construction of the Parent Polypeptide as Set Forthin SEQ ID NO: 2

Construction of a polypeptide having the amino acid sequence set forthin SEQ ID NO: 2 (the parent polypeptide). The polypeptide is a modifiedalpha-amylase (EC 3.2.1.1) belonging to the glycoside hydrolase GH13Family, Subfamily 5.

Example 2: Generation of Variants

Using the parent alpha-amylase having the amino acid sequence as setforth in SEQ ID NO: 2, the variants of the present invention wereconstructed. The variants were prepared by standard procedures, which inbrief is; introducing an amino acid substitution in SEQ ID NO: 2 bysite-directed mutagenesis into the gene, transforming Bacillus subtilishost cells with the mutated gene, fermenting the transformed cells (e.g.as described in Example 1 of WO 2004/111220), and purifying the variantsfrom the fermentation broth. The reference amylase, i.e. the parentalpha-amylase, having the amino acid sequence as set forth in SEQ ID NO:2, were produced recombinantly in Bacillus subtilis in a similar manner.

A library of variants were generated where different combinations ofmodifications in the following positions were made: H1, H2, G7, I9, Q11,N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59, A60, L63, T81, E84,E86, R90, Q98, V104, G109, A111, F113, R116, Q118, Q125, R127, E130,S132, G133, T134, Y135, Q136, A139, G142, N144, G149, R158, Y160, D163,W167, Q169, S170, R171, Q172, L173, A174, N175, R176, Y178, R181, G182,A186, W187, N195, M202, Y203, A204, V206, D209, H210, E212, T227, L235,V238, M246, Q256, K259, V264, A265, E266, Y267, K269, N270, L272, G273,A274, L275, E276, W284, M286, V291, L293, Y295, Y298, Q299, N302, S303,S304, N306, R310, N311, N314, G315, L317, Q319, R320, S323, F328, G337,A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395, R400, D406, W408,V410, S431, G435, W439, R444, Q445, R458, N465, Q466, W469, F473, G476and V481, using SEQ ID NO: 1 for numbering.

The generated variants are listed in the following examples.

Example 3: Amylase Activity Assay with CS-28 Swatches

Purified amylase variants were initially diluted to 0.1 mg/ml(concentrations based on absorbance at 280 nm) with 0.01% Triton X-100,1 mM CaCl₂. Further enzyme dilutions are in 0.01% Triton X-100.

In microtiter plates containing a CS-28 rice starch substrate swatch ineach of the 96 wells, 50 μl diluted amylase (final concentrations inwells of 0.04, 0.02 and 0.01 ppm) was mixed with 150 μl Model A washsolution (final concentration in well of 3.45 g/l Model A detergent, 21°dH water hardness (molar ratio Ca:Mg:HCO3=4:1:10)). After 10-15 minincubation at room temperature with agitation, 20 μl supernatant wastransferred to another 96 well microtiter plate to determine solubilizedamount of starch fragments. To hydrolyze the starch fragments toglucose, 10 μl 0.5 mg/ml glucoamylase was added and the plate wasincubated 10 min at room temperature with agitation. Then 140 μlGOD-Perid reagent (0.1 M potassium phosphate, pH 7, 0.6 g/l glucoseoxidase (Sigma G6125), 20 mg/l peroxidase (Sigma P8125), 1 g/l ABTS(Roche 102946)) was added, and glucose concentration was assumed to beproportional to absorbance read in a plate reader at 420 nm after 15 minincubation. For each sample duplicates of 3 concentrations (0.04, 0.02and 0.01 ppm) are tested. A reference sample (SEQ ID NO: 2) was includedon all plates. A dose-response curve was fitted (least squares method)for each sample to a 4-parameter logistic expression:

A420=A420 min+(A420 max−A420 min)/(1+(C/C50){circumflex over ( )}Slope)

where A420 is measured absorbance, A420 min is a minimum absorbance,A420 max is a maximum absorbance, C is the amylase concentration, C50 isthe amylase concentration giving an absorbance of 0.5*(A420 min+A420max), and Slope is a steepness parameter. A420 min, A420 max and Slopeare assumed to be identical for all samples on a plate, whereas C50 isfitted for each sample. Dose Improvement Factor (Dose IF) for a sampleis calculated as:

Dose IF(sample)=C50(reference)/C50(sample)

TABLE 1 Model A detergent (percentages given in w/w) Content Fractionactive Compound ingredients component MGDA- H₃ (Trilon 33%  59% MGranules SG) Sodium citrate 20% 100% Sodium carbonate 20% 100% Sodiumsilicate  6%  80% Sodium sulfate 12% 100% Acusol 588G  5%  92% Non-ionicsurfactant  5% 100% (Surfac 23-6.5 (added as liquid)

Dose IF of the variants according to the invention obtained by themethod described above are shown in Table 2 below. The test resultsclearly demonstrate that the variants have improved relative activity tothe reference amylase, i.e. the parent alpha-amylase.

TABLE 2 Relative specific activity of variants Modifications in SEQ IDNO: 2 IF SEQ ID NO: 2 1.0 A60T 1.4 Y135H 1.4 Q169E 1.4 W48Y 1.3 A174*1.1 M246L 1.5 R90Q 1.4 Y203L + V206L 1.3 Q169F + S170A 1.1 S132D + G133D1.1 N195F + Q365C 2.6 N195F + Q365S 2.1 N195F + Q365M 2.1 N195F + Q365K1.8 R127L + Q365S 2.0 R127N + Q365S 2.0 R127L + Q365C 1.9 R171L + Q365S1.4 R171H + Q365C 1.6 Y267F + Q365S 1.5 F328L + Q365S 1.5 I9M + N195F1.7 N195F + M286L 1.1 T134P + N195F 1.1 S170* + R171* + A174* 2.6V264I + A265G + Y267F 2.3 Q169* + S170* + A174* 2.2 M246L + L275V +Q365S 1.5 V264I + V291I + Q365S 2.1 V264I + V291T + Q365K 2.1 V264I +F328L + Q365C 2.0 I9M + N195F + Q365C 1.9 Q11H + Y267F + Q365C 1.7 I9M +N195F + Q365S 1.7 V264I + Y267F + Q365K 1.6 R127L + M246V + Q365S 1.6A60S + R171Y + Q365S 1.4 R127N + M246T + L275V 1.4 V104A + N195F + Q365S1.4 V264I + F328L + Q365S 1.4 R171H + N302H + Q365S 1.4 V264I + Y267F +Q365S 1.3 A174* + N175* + Y178F 1.3 V264I + A265G + Q365C 1.3 R171L +N302H + Q365C 1.1 R171L + M246V + Q365C 1.1 R171Y + M246L + Q365S 1.1V264T + Y267F + Q365S 1.1 I9M + Y160H + N195F + Q365S 2.7 I9M + R127N +N195F + Q365S 2.1 R127H + L235I + Q365S + G435C 2.5 I9M + R171H +N195F + Q365S 2.5 I9M + A186H + N195F + Q365S 2.4 I9M + R171L + N195F +Q365S 2.3 Q169* + S170* + R171* + A174* 2.3 M246L + L275V + Q365S +D406H 2.3 I9M + G184K + N195F + Q365S 2.2 I9M + R127N + N195F + Q365S2.2 R127N + M246L + L275V + Q365S 2.2 R171H + M246L + L275V + Q365S 2.1R127H + M246I + Q365C + V481A 2.0 R127N + M246L + L275V + Q365S 2.0Y267F + V291A + F328L + Q365C 2.0 I9M + A186E + N195F + Q365S 1.9 E86L +R171L + N306R + Q365S 1.8 V264I + Y267F + F328L + Q365S 1.7 I9M +N195F + V291A + Q365S 1.7 R127L + L235I + M246I + Q365C 1.7 Y267F +V291A + F328L + Q365S 1.6 V264I + Y267F + F328L + Q365K 1.6 Q11H +V264I + A265G + Q365C 1.5 I9M + R171H + N195F + Q365S 1.4 W187M +N195F + H210K + Q365S 1.4 R127H + M246T + L275A + Q365S 1.4 I9M +A111T + N195F + Q365S 1.3 I9M + Y58F + N195F + Q365S 1.3 V264T + V291A +F328L + Q365S 1.2 V264I + A265G + V291A + Q365C 1.1 V264I + A265G +Y267F + Q365C 1.1 V264I + Y267F + V291A + F328L + Q365S 2.5 R127N +R171H + M246L + L275V + Q365S 1.3 V264I + A265G + V291A + F328L + Q365S2.4 R127N + M246I + A265G + Y267H + Q365S 2.3 E130I + T134A + R181N +N195F + Q365S 2.2 R127N + M246L + L275V + N306R + Q365S 2.0 I9M +N195F + V206L + D209N + Q365S 2.0 R116S + S132F + R181N + N195F + Q365S1.9 E130T + T134Y + A186K + N195F + Q365S 1.9 A37V + V264I + Y267F +F328L + Q365C 1.8 Q11H + V264I + Y267F + F328L + Q365C 1.8 V264I +Y267F + V291T + F328L + Q365S 1.8 Q169* + R171* + Q172* + L173* + A174*1.8 R116Y + S132P + Q136N + N195F + Q365S 1.8 N195F + G337D + Q365S +P375K + D377Y 1.7 V264I + A265G + Y267F + F328L + Q365S 1.7 19M +N195F + K269Q + A274K + Q365S 1.7 E130D + T134Y + R181H + N195F + Q365S1.6 R116N + S132M + Q136R + N195F + Q365S 1.5 N195F + G337D + Q365S +P375K + D377N 1.4 R127N + R171L + M246L + L275V + Q365S 1.2 N195F +G337E + Q365S + P375K + D377N 1.3 Q11H + V264I + A265G + Y267F + Q365C1.3 R158Q + Y160N + W187M + N195F + Q365S 1.3 V264I + Y267F + Q365K +W408H + V41 OI 1.2 R127N + R171Y + M246L + L275V + Q365S 1.2 N54Q +F113T + R171H + N302A + Q365C 1.2 V264A + Y267F + V291T + F328L + Q365C1.1 Q169* + S170* + R171 * + Q172* + L173* + A174* 2.6 R116K + S132L +Q136R + R181C + N195F + Q365S 2.3 V264T + A265G + Y267F + V291A +F328L + Q365C 2.2 E130N + T134S + R181N + A186K + N195F + Q365S 2.0E130V + T134N + R181Y + A186K + N195F + Q365S 2.0 E130D + T134W +R181KA186E + N195F + Q365S 1.9 R116N + S132A + Q136D + R181K + N195F +Q365S 1.9 E130S + T134E + R181S + A186T + N195F + Q365S 1.9 R116C +S132A + Q136S + R181A + N195F + Q365S 1.8 R116K + S132P + Q136D +R181Q + N195F + Q365S 1.8 R127H + R171H + M246L + L275V + N306R + Q365S1.8 E130L + T134N + R181W + A186T + N195F + Q365S 1.8 R116C + S132M +Q136S + R181Q + N195F + Q365S 1.7 R116S + Y135H + Q136G + R181T +N195F + Q365S 1.7 R116S + S132L + Q136E + R181G + N195F + Q365S 1.6N16E + N195F + G337E + Q365S + P375T + D377H 1.5 V264I + A265G + Y267F +V2911 + F328L + Q365S 1.4 N16E + N195F + G337D + Q365S + P375K + D377N1.3 V264I + A265G + Y267F + F328L + Q365S + Q395K 1.2 E130N + T134C +R181Q + A186N + N195F + Q365S 1.2 V264I + A265G + Y267F + V291A +F328L + Q365C 1.1 W167* + Q169* + S170* + R171* + Q172* + L173* + A174*3.2 W167* + Q169* + S170* + R171* + Q172* + L173* + A174* + N175* 1.1R116K + S132P + Q136D + R181Q + N195F + A204V + Q365S 2.8 N16Y + Y160H +N195F + L275I + S304G + G337E + Q365S 2.6 E130K + S132T + T134Y +R181T + A186K + N195F + Q365S 2.5 E130Y + S132T + T134S + R181E +A186D + N195F + Q365S 2.0 A139T + R158S + Y160D + W187M + N195F +H210N + Q365S 1.2 I9M + R171L + N195F + M246L + K269M + W284R + Y298N +Q365S 1.6 H1* + N54S + V56T + G109A + R171H + N195F + V206L + K391A 1.3W167* + Q169* + S170* + R171 * + Q172* + L173* + A174* + N175* + R176*1.8 H1* + G59A + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A1.7 H1* + Y58F + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A1.3 H1* + N54S + G109A + Q169E + Q172K + A174* + N195F + V206L + L293I +K391A 1.9 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +D209N + K391A 1.8 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195D + V206L + K391A 1.8 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + V206L + K391A 1.5 N16H + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + K391A 1.4 H1* + N54S + V56T + G109A +Q169E + Q172K + A174* + N195H + V206L + K391A 1.3 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + V206L + M246L + K391A 2.8Y160H + E212D + E276N + W284Y + R320S + Q365S + W408H + W439T + W469N +F473S + G476K 2.8 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + L275l + K391A 2.4 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + G337E + K391A 2.3 H1* + N16Y + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 2.3 H1* +N25K + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +K391A 2.2 H1* + N54S + V56T + G109A + R127T + Q169E + Q172K + A174* +N195F + V206L + K391A 2.2 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + V206L + D209N + K391A 2.2 H1* + N54S + V56T + G109A +R127N + Q169E + Q172K + A174* + N195F + V206L + K391A 2.1 H1* + N54S +V56T + L63F + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 2.1H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +Q365S + K391A 2.1 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + L275V + K391A 2.1 H1* + N19D + N54S + V56T + G109A +Q169E + Q172K + A174* + N195F + V206L + K391A 2.1 A60S + Y160H + E212D +E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K 2.1 H1* +N54S + V56T + G109A + Q169E + R171 L + Q172K + A174* + N195F + V206L +K391A 2.0 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + G337D + K391A 2.0 H1* + N25M + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + K391A 2.0 H1* + N54S + V56T + T81A +G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 2.0 H1* + N25T +N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 2.0H1* + N54S + V56T + G109A + Y160H + Q169E + Q172K + A174* + N195F +V206L + K391A 1.9 H1* + N54S + V56T + R90N + G109A + Q169E + Q172K +A174* + N195F + V206L + K391A 2.0 H1* + N54S + V56T + G109A + Q169E +R171 L + Q172K + A174* + N195F + V206L + K391A 2.0 E86L + Y160H +E212D + E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K1.9 H1* + N54S + V56T + G109A + R158C + Q169E + Q172K + A174* + N195F +V206L + K391A 1.9 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + L293V + K391A 1.9 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + L272I + K391A 1.9 H1* + N54S + V56T +G109A + R158H + Q169E + Q172K + A174* + N195F + V206L + K391A 1.9 H1* +G109A + K391A + N16Y + N54S + V56T + Q169E + Q172K + A174* + N195F +V206L 1.9 H1* + N54S + V56T + G109A + R158S + Q169E + Q172K + A174* +N195F + V206L + K391A 1.9 R127N + Y160H + E212D + E276N + W284Y +R320S + W408H + W439T + W469N + F473S + G476K 1.9 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + V206L + A274S + K391A 1.9 H1* +N54S + V56T + G109A + Q169E + Q172K + A174* + A186E + N195F + V206L +K391A 1.7 H1* + N54S + V56T + R90H + G109A + Q169E + Q172K + A174* +N195F + V206L + K391A 1.9 I9M + Y160H + E212D + E276N + W284Y + R320S +W408H + W439T + W469N + F473S + G476K 1.8 Y160H + Q172S + E212D +E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.8 H1* +N54S + V56T + G109A + Q169E + Q172K + A174* + G184H + N195F + V206L +K391A 1.8 H1* + N54S + V56T + A60S + G109A + Q169E + Q172K + A174* +N195F + V206L + K391A + 1.8 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + V206L + Q365C + K391A 1.8 H1* + N54S + V56T + G109A +R116N + Q169E + Q172K + A174* + N195F + V206L + K391A 1.8 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + N306H + K391A 1.8H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +N302H + K391A 1.8 H1* + N54S + V56T + G109A + Q169E + R171Y + Q172K +A174* + N195F + V206L + K391A 1.8 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + Q365E + K391A 1.8 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + A186H + N195F + V206L + K391A 1.8 A60S +Y160H + E212D + E276N + W284Y + R320S + W408H + W439T + W469N + F473S +G476K 1.8 H1* + N54S + V56T + A60S + G109A + Q169E + Q172K + A174* +N195F + V206L + K391A 1.8 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + R181S + N195F + V206L + K391A 1.7 H1* + N54S + V56T + G109A +Q169E + Q172S + A174* + N175S + N195F + V206L + K391A 1.7 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + L275V + K391A 1.7H1* + N54S + V56T + E86L + G109A + Q169E + Q172K + A174* + N195F +V206L + K391A 1.7 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + N302K + K391A 1.7 H1* + N54S + V56T + G109A + R127L +Q169E + Q172K + A174* + N195F + V206L + K391A 1.7 H1* + N54S + V56T +G109A + Q169E + R171Y + Q172K + A174* + N195F + V206L + K391A 1.7 H1* +N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + V291A +K391A 1.7 H1* + N54S + V56T + G109A + R116F + Q169E + Q172K + A174* +N195F + V206L + K391A 1.7 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + V206L + N306R + K391A 1.7 Y160H + R171L + E212D +E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.7 H1* +G109A + K391A + N54S + V56T + Y160H + Q169E + Q172K + A174* + N195F +V206L 1.7 H1* + N54S + V56T + G109A + Q118N + Q169E + Q172K + A174* +N195F + V206L + K391A 1.6 H1* + N54S + V56T + G109A + T134P + Q169E +Q172K + A174* + N195F + V206L + K391A 1.6 H1* + N54S + V56T + G109A +Q169E + Q172K + A174*N195F + V206L + Q299Y + K391A 1.6 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + N306K + K391A 1.6H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +Q365A + K391A 1.6 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + Q299N + K391A 1.6 Y160H + E212D + L275V + E276N +W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.6 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + N306Q + K391A 1.6H1* + W43Y + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + K391A 1.6 H1* + N54S + V56T + G109A + R127L + Q169E + Q172K +A174* + N195F + V206L + K391A 1.6 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + L275V + K391A 1.5 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + V206L + N311R + K391A 1.5Y160H + E212D + M246L + E276N + W284Y + R320S + W408H + W439T + W469N +F473S + G476K 1.5 H1* + N54S + V56T + E84Q + G109A + Q169E + Q172K +A174* + N195F + V206L + K391A 1.5 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + N306H + K391A 1.5 H1* + N54S + V56T +G109A + R158K + Q169E + Q172K + A174* + N195F + V206L + K391A 1.5 H1* +N54S + V56T + G109A + R127N + Q169E + Q172K + A174* + N195F + V206L +K391A 1.5 V56T + Y160H + E212D + E276N + W284Y + R320S + W408H + W439T +W469N + F473S + G476K 1.5 N54S + Y160H + E212D + E276N + W284Y + R320S +W408H + W439T + W469N + F473S + G476K 1.5 H1* + N54S + V56T + E86L +G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 1.5 Y160H +E212D + Q256H + E276N + W284Y + R320S + W408H + W439T + W469N + F473S +G476K 1.5 H1* + N54S + V56T + G109A + Q125G + Q169E + Q172K + A174* +N195F + V206L + K391A 1.4 H1* + Y160H + E212D + E276N + W284Y + R320S +W408H + W439T + W469N + F473S + G476K 1.4 H1* + N54S + V56T + G109A +Q169E + Q172K + A174* + N195F + V206L + N306R + K391A 1.4 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + N311K + K391A 1.4H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +K269M + K391A 1.4 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +G184K + N195F + V206L + K391A 1.4 Y160H + E212D + K259G + E276N +W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.4 Y160H +A174S + E212D + E276N + W284Y + R320S + W408H + W439T + W469N + F473S +G476K 1.3 Y160H + N195Y + E212D + E276N + W284Y + R320S + W408H +W439T + W469N + F473S + G476K 1.3 Y160H + Q169E + E212D + E276N +W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.3 E86L + Y160H +E212D + E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K1.3 H1* + N16H + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + K391A 1.3 Y160H + E212D + E276N + W284Y + N306Y + R320S +W408H + W439T + W469N + F473S + G476K 1.3 H1* + N54S + V56T + G109A +Q169E + Q172K + A174* + N195F + V206L + Q365S + K391A 1.2 G7A + F113N +R116N + E276N + S304G + R320S + Q345N + W439R + N465G + Q466S + W469Y1.2 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +D377H + K391A 1.2 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + V238A + K391A 1.2 Y160H + N195F + E212D + E276N +W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.2 Y160H +E212D + Q256N + E276N + W284Y + R320S + W408H + W439T + W469N + F473S +G476K 1.2 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + Q365H + K391A 1.2 Y160H + N195Q + E212D + E276N + W284Y +R320S + W408H + W439T + W469N + F473S + G476K 1.2 Y160H + R171Y +E212D + E276N + W284Y + R320S + W408H + W439T + W469N + F473S + G476K1.2 G109A + Y160H + E212D + E276N + W284Y + R320S + W408H + W439T +W469N + F473S + G476K 1.2 Y160H + E212D + E276N + W284Y + N306R +R320S + W408H + W439T + W469N + F473S + G476K 1.2 H1 * + N54S + V56T +G109A + R127F + Q169E + Q172K + A174* + N195F + V206L + K391A 1.1 H1* +N54S + V56T + G109A + Q169E + Q172N + L173K + A174* + N175S + N195F +V206L + K391A 2.2 Y160H + E212D + E276N + W284Y + S304G + N306R +R320S + W408H + W439T + W469N + F473S + G476K 2.2 H1* + N54S + V56T +G109A + G142H + N144H + Q169E + Q172K + A174* + N195F + V206L + K391A2.0 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +N302Q + Q385L + K391A 1.9 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + V206L + N306R + Q385L + K391A 1.9 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + G184K + N195F + V206L + T227N + K391A1.8 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +T227K + K269R + K391A 1.8 H1* + N54S + V56I + G109A + R127A + Q169E +Q172K + A174* + N195F + V206L + Q385L + K391A 1.8 Y160H + E212D +Q256H + K259H + E276N + W284Y + R320S + W408H + W439T + W469N + F473S +G476K 1.6 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + G315N + Q385L + K391A 1.6 H1* + N54S + V56T + G109A + Q169E +Q172N + A174* + N175S + N195F + V206L + K391A + S431F 1.5 Y160H +E212D + E276N + W284Y + S304G + N306Y + R320S + W408H + W439T + W469N +F473S + G476K 1.5 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + L235l + V238A + K391A 1.4 H1* + N54S + V56T + G109A +Q172S + L173K + A174S + N175* + R176A + N195F + V206L + K391A 1.4 H1* +N54S + V56T + G109A + R116D + Q118T + Q169E + Q172K + A174* + N195F +V206L + K391A 1.3 V56T + Y160H + A174N + E212D + E276N + W284Y + R320S +W408H + W439T + W469N + F473S + G476K 1.2 H1* + N54S + V56T + R90Q +G109A + Q169E + Q172K + A174* + N195F + V206L + E266V + Q385L + K391A2.1 H1* + N16H + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V20 + 6L + Q385L + K391A 2.1 H1* + N54S + V56T + G109A + F113A + R116D +Q118T + Q169E + Q172K + A174* + N195F + V206L + K391A 1.8 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + Y298E + N302K +S303G + K391A 1.5 H1* + N54S + V56T + G109A + R116H + Q169E + Q172K +A174* + N195F + V206L + N314Q + Q385L + K391A 1.4 H1* + I9M + N16Y +N54S + V56T + G1O9A + R116Y + Q118T + Q125S + Q136E + Y160H + Q169E +Q172K + A174* + 1.3 R181H + N195F + M202L + V206L + R310N + R320Q +Q365S + K391A + R400S + W408H + R444T + Q445S + Q466S

Example 4: Assessment of Wash Performance of Alpha-Amylase VariantsUsing Automatic Mechanical Stress Assay (AMSA) for Automatic Dish Wash

Washing experiments are performed in order to assess the washperformance of selected alpha-amylase variants in dishwash detergentcompositions. The alpha-amylase variants of the present application maybe tested using the Automatic Mechanical Stress Assay (AMSA). With theAMSA, the wash performance of many small volume enzyme-detergentsolutions can be examined. The AMSA plate has a number of slots for testsolutions and a lid that firmly squeezes the dish wash monitor to bewashed against the slot openings. During the wash, the plate, testsolutions, dishwash monitor and lid are vigorously shaken to bring thetest solution in contact with the soiled dishwash monitor and applymechanical stress in a regular, periodic oscillating manner. For furtherdescription see WO 02/42740 especially the paragraph “Special methodembodiments” at page 23-24.

The experiment may be conducted under the experimental conditions asspecified in the Table 3 below:

TABLE 3 Experimental condition Detergent ADW model detergent with bleach(Table 5) Detergent dosage 3.94 g/L Test solution volume 160 micro L pHAs is Wash time 20 minutes Temperature 45° C. Water hardness 21° dH(ratio Ca:Mg:HCO3 = 4:1:10) Enzyme concentration in 0.35 mg enzymeprotein/L test solution Test material DM-277 (double soiled Mix starch)

Water hardness was adjusted to 21° dH by addition of CaCl₂, MgCl₂, andNaHCO₃ (Ca²⁺:Mg²⁺:HCO_(3*)=4:1:10) to Milli-Q water.

The wash performance is measured as the brightness of the color of thedish wash monitor. Brightness can also be expressed as the intensity ofthe light reflected from the sample when illuminated with white light.When the sample is stained the intensity of the reflected light islower, than that of a clean sample. Therefore, the intensity of thereflected light can be used to measure wash performance.

Color measurements are made with a professional flatbed scanner, whichis used to capture an image of the washed textile. To extract a valuefor the light intensity from the scanned images, 24-bit pixel valuesfrom the image are converted into values for red, green and blue (RGB).The intensity value (Int) is calculated by adding the RGB valuestogether as vectors and then taking the length of the resulting vector:

Int+√{square root over (r ² +g ² +b ²)}

General Wash Performance Description

A test solution comprising water hardness adjusted water (21° dH, ratioCa:Mg:HCO3=4:1:10), 3.94 g/L detergent, as described below, and theenzyme of the invention, e.g. at concentration of 0.35 mg enzymeprotein/L, was prepared. Melamine tiles stained with starch (e.g. DM-277(double soiled Mix starch) from Center For Testmaterials BV, P.O. Box120, 3133 KT, Vlaardingen, The Netherlands), used as dishwash monitors,were added to the AMSA plate and washed for 20 minutes at 45° C. Afterthorough rinse under cold running tap water and drying in the dark, thelight intensity of the stained tiles was subsequently measured as ameasure for wash performance using a professional flatbed scanner (KodakiQsmart, Kodak, Midtager 29, DK-2605 Brøndby, Denmark).

The wash performance was compared to that of the parent alpha-amylase,with the performance result of the parent alpha-amylase is assigned thevalue of 1.0 and the results of the variants are compared to this value(improvement Factor (IF) value).

The wash performance of the variants according to the invention obtainedby AMSA are the following:

TABLE 4 AMSA Performance Improvement Factor (IF) Dataset I Modificationsin SEQ ID NO: 2 IF SEQ ID NO: 2 1.0 Y295F 1.9 M246L 1.5 M202L 2.5R181S + G184S 1.5 F113Q + R116H 1.5 V264I + L357F 1.3 Y267F + Q365S 1.8R171H 1.6 R171Y + N306K 1.2 R171H + Q365C 2.5 R171L + Q365S 2.1 R171H +N302H + Q365S 2.1 R171L + N302H + Q365C 2.0 R171Y + + M246L + Q365S 1.7F328L + Q365S 1.6 R127L 1.1 R127N + Q365S 1.2 R127L + Q365C 1.3 R127L +Q365S 1.2 R127L + M246V + Q365S 1.3 A186D + N195F 1.2 V264I + Q365C 1.1I9M + N195F + Q365C 2.7 V264I + A265G + Y267F 2.1 V264I + V291T + Q365K1.8 A60S + R171Y + Q365S 1.8 V264I + Y267F + Q365K 1.7 V264I + F328L +Q365C 1.7 M246L + L275V + Q365S 1.7 V264T + Y267F + Q365S 1.7 V264I +F328L + Q365S 1.6 Q11H + Y267F + Q365C 1.5 V104A + N195F + Q365S 1.3V264I + V291I + Q365S 1.3 V264I + Y267F + Q365S 1.3 F113T + R171K +G273V 1.1 Q169* + S170* + A174* 1.1 I9M + R171H + N195F + Q365S 1.6I9M + R127N + N195F + Q365S 1.6 E86L + R171L + N306R + Q365S 2.3 I9M +A186E + N195F + Q365S 2.3 I9M + Y160H + N195F + Q365S 2.1 I9M + A186H +N195F + Q365S 2.1 R171H + M246L + L275V + Q365S 1.4 I9M + N195F +V291A + Q365S 1.9 I9M + G184K + N195F + Q365S 1.8 M246L + L275V +Q365S + D406H 1.8 Y267F + V291A + F328L + Q365S 1.8 Y267F + V291A +F328L + Q365C 1.7 V264I + Y267F + F328L + Q365K 1.7 I9M + R171L +N195F + Q365S 1.6 Q11H + V264I + A265G + Q365C 1.6 V264T + V291A +F328L + Q365S 1.4 V264I + Y267F + F328L + Q365S 1.3 R127N + M246L +L275V + Q365S 1.3 I9M + N195F + N270G + Q365S 1.2 I9M + N195F + N270P +Q365S 1.2 V264T + Y267L + F328L + Q365C 1.2 V264I + A265G + V291A +Q365C 1.2 W187M + N195F + H210K + Q365S 1.2 V264I + Y267F + F328L +Q365C 1.1 Q169* + S170* + R171* + A174* 1.1 I9M + N195F + V206L +D209N + Q365S 2.3 N195F + G337D + Q365S + P375K + D377N 2.0 V264I +Y267F + V291T + F328L + Q365S 1.9 N195F + G337E + Q365S + P375K + D377N1.8 N195F + G337D + Q365S + P375K + D377Y 1.7 V264I + A265G + V291A +F328L + Q365S 1.7 I9M + N195F + K269Q + A274K + Q365S 1.7 V264I +Y267F + Q365K + W408H + V410I 1.6 E130D + T134Y + R181H + N195F + Q365S1.6 V264I + Y267F + V291A + F328L + Q365S 1.6 E130I + T134A + R181N +N195F + Q365S 1.5 R116Y + S132P + Q136N + N195F + Q365S 1.5 R116N +S132M + Q136R + N195F + Q365S 1.5 V264I + A265G + Y267F + F328L + Q365S1.4 Q11H + V264I + Y267F + F328L + Q365C 1.4 Q11H + V264I + A265G +Y267F + Q365C 1.4 E130T + T134Y + A186K + N195F + Q365S 1.3 R116S +S132F + R181N + N195F + Q365S 1.2 V264A + Y267F + V291T + F328L + Q365C1.2 N54Q + F113T + R171H + N302A + Q365C 1.2 R127N + R171L + M246L +L275V + Q365S 1.2 V264T + A265G + Y267L + F328L + Q365C 1.1 V264I +Y267F + V291A + F328L + Q365C 1.1 N16E + N195F + G337E + Q365S + P375T +D377H 1.9 E130D + T134W + R181K + A186E + N195F + Q365S 1.7 E130F +T134R + R181S + A186D + N195F + Q365S 1.5 V264T + A265G + Y267F +V291A + F328L + Q365C 1.5 E130V + T134N + R181Y + A186K + N195F + Q365S1.4 R116N + S132A + Q136D + R181K + N195F + Q365S 1.4 E130L + T134N +R181W + A186T + N195F + Q365S 1.3 V264I + A265G + Y267F + F328L +Q365S + Q395K 1.3 N16E + N195F + G337D + Q365S + P375K + D377N 1.3E130N + T134C + R181Q + A186N + N195F + Q365S 1.2 R116S + Y135H +Q136G + R181T + N195F + Q365S 1.2 R116K + S132L + Q136R + R181C +N195F + Q365S 1.2 V264I + A265G + Y267F + V291I + F328L + Q365S 1.2E130S + T134E + R181S + A186T + N195F + Q365S 1.1 N16Y + Y160H + N195F +L275I + S304G + G337E + Q365S 1.6 A139T + R158S + Y160D + W187M +N195F + H210N + Q365S 1.2 R116K + S132P + Q136D + R181Q + N195F +A204V + Q365S 1.2 E130Y + S132T + T134S + R181E + A186D + N195F + Q365S1.2 I9M + R171L + N195F + M246L + K269M + W284R + Y298N + Q365S 2.3Q98N + F113Y + R158Q + Y160F + S304Q + R320S + W439R + F473P 1.5 H1* +N54S + V56T + G109A + R171H + N195F + V206L + K391A 1.1 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + V206L + K391A 1.3 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + M202L + V206L + K391A 1.8H1* + N16H + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +V206L + K391A 3.4 Y160H + M202L + E212D + E276N + W284Y + R320S +W408H + W439T + W469N + F473S + G476K 3.3 Y160H + M202L + E212D +E276N + W284Y + R320S + W408H + W439T + W469N + F473I + G476K 1.1Y160H + E212D + E276N + W284Y + R320S + Q365S + W408H + W439T + W469N +F473S + G476K 1.4 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + V206L + Q365S + K391A 1.4 E86L + Y160H + E212D + E276N + W284Y +R320S + W408H + W439T + W469N + F473S + G476K 1.1 H1* + N16Y + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 1.1 H1* +N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + N306R +K391A 1.1 H1* + N54S + V56T + G109A + Y160H + Q169E + Q172K + A174* +N195F + M202L + V206L + K391A 2.3 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + M202L + V206L + R320S + K391A 2.2 H1* + N16Y +N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + M202L + V206L +K391A 2.2 H1* + I9M + N54S + V56T + G109A + Q169E + Q172K + A174* +N195F + M202L + V206L + K391A 1.5 I9M + Y160H + M202L + E212D + E276N +W284Y + R320S + W408H + W439T + W469N + F473S + G476K 1.2 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + M202L + V206L + Q365S +K391A 1.1 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +M202L + V206L + W284Y + K391A 1.1 H1* + I9M + N54S + V56T + G109A +G149A + Q169E + Q172K + A174* + G182T + N195F + M202L + 2.3 V206L +Y295F + Q299Y + R320K + S323T + A339S + Q345R + Q365S + K391A + R458KH1* + I9M + N54S + V56T + G109A + F113G + R116H + D163H + Q169E +R171H + Q172K + A174S + 1.2 G182T + N195F + M202L + V206L + M246L +L275V + A288V + L317V + Q319H + H324K + Q365S + K391A H1* + I9M + N16Y +N54S + V56T + G109A + R116Y + Q118T + Q125S + Q136E + Y160H + Q169E +2.0 Q172K + A174* + R181H + N195F + M202L + V206L + R310N + R320Q +Q365S + K391A + R400S + W408H + R444T + Q445S + Q466S

Example 5: Assessment of Wash Performance of Alpha-Amylase VariantsUsing Automatic Dish Wash (ADW)

In order to assess the wash performance of the alpha-amylase variants ofthe present invention in a detergent base composition, washingexperiments may be performed using full scale Automatic Dish Wash (ADW).The full scale ADW setup is used for testing the wash performance ofpolypeptides in test conditions mimicking a regular consumer setup.

In the present study, 40° C. and 45° C. wash programs using a Miele GSL2dishwasher were used.

General Wash Performance Description.

Melamine tiles stained with starch (DM-277) (from Center For Testmaterials BV, P.O. Box 120, 3133 KT, Vlaardingen, The Netherlands) wereused as test material and washed at “R40° C./8 min/KI55° C.” and “R45°C./8 min/KI55° C.” program in presence of 50 g of IKW Ballast soil usingwater with 21° dH, as specified below (see Tables 5 and 6). Two tiles ofeach stain type were added to each automatic dishwasher. Four replicateswere carried out and an average for each test condition was calculated.

After approximately 2 minutes of running the machine program (when thedetergent dispenser opened), the detergent and the amylase polypeptideof the invention were added. The full-scale wash performance experimentswere conducted under the experimental conditions specified below:

TABLE 5 Experimental conditions Detergent ADW model detergent (withbleach) (see Table 6) Detergent dosage 21.27 g/wash pH As is Washprogram 40° C./8/KI55C or 45° C./8/KI55C Water hardness 21° dH Amylaseconcentration As described below. in test Typically, 0.9 mgpolypeptide/wash using program 45° C./8/KI55C or 1.5 mg polypeptide/wash using program 40° C./8/KI55C Test material Melamine plates (MixedStarch) DM-277

TABLE 6 ADW model detergent (with bleach) (percentages given in w/w)Content Fraction active Compound ingredients component MGDA-H₃ (Trilon M29% 59% Granules SG) Sodium citrate 17% 100%  Sodium carbonate 17% 100% Sodium percarbonate 10% 88% Sodium silicate  5% 80% Sodium sulfate 10%100%  Acusol 588G  5% 92% TAED  3% 92% Non-ionic surfactant  4% 100% (Surfac 23-6.5 (added as liquid)

The wash performance was measured as difference in remission for themelamine tiles. The remission measurements were made with a Color-Eye7000 (CE7000) used for taking spectra and performing calculations ofremission and/or colour difference. The remission was measured at 460 nmwith no UV light in the illuminant.

The term “improved wash performance” of the present experiment wasdefined as displaying an alteration of the wash performance of a variantof the present invention relative to the wash performance of thepolypeptide having an amino acid sequence as set forth in SEQ ID NO: 2.The alteration may e.g. be seen as increased stain removal. Improvedwash performance was determined as described above. The wash performancewas considered to be improved if the Improvement Factor (IF) was atleast 1.1, preferably at least 1.2 in one or more of the conditionslisted above; i.e. either at 400/8/K1550 or 450/8/K1550.

The wash performance of the variants according to the invention obtainedby full scale wash are the following:

TABLE 7 FSW dataset I (40° C./8/KI55C-1.5 mg protein/wash) Modificationsin SEQ ID NO: 2 IF SEQ ID NO: 2 1.0 V264I + A265G + Y267F 1.6 R171L +Q365S 1.4 Y267F + Q365S 1.3 R127L + Q365S 1.2 V264I + F328L + Q365C 1.2M246L + L275V + Q365S + D406H 1.2 Q11H + V264I + A265G + Q365C 1.1R127N + R171H + M246L + L275V + Q365S 1.8 V264I + Y267F + V291T +F328L + Q365S 1.8 Y267F + V291A + F328L + Q365S 1.4 V264I + Y267F +F328L + Q365K 1.4 R127H + L235I + Q365S + G435C 1.2 N195F + Q365S 1.3I9M + N195F + Q365C 1.5 I9M + R127N + N195F + Q365S 1.6 I9M + Y160H +N195F + Q365S 1.6 I9M + A186E + N195F + Q365S 1.5 I9M + R171H + N195F +Q365S 1.4 I9M + A186H + N195F + Q365S 1.2 I9M + G184K + N195F + Q365S1.4 N195F + G337E + Q365S + P375K + D377N 1.5 N195F + G337D + Q365S +P375K + D377Y 1.4 E130I + T134A + R181N + N195F + Q365S 1.3 R116N +S132A + Q136D + R181K + N195F + Q365S 1.1 N16E + N195F + G337E + Q365S +P375T + D377H 1.7 E130D + T134W + R181K + A186E + N195F + Q365S 1.6E130Y + S132T + T134S + R181E + A186D + N195F + Q365S 1.5 R116K +S132P + Q136D + R181Q + N195F + A204V + Q365S 1.8 N16Y + Y160H + N195F +L275I + S304G + G337E + Q365S 2.1 I9M + R171L + N195F + M246L + K269M +W284R + Y298N + Q365S 1.4 Y160H + M202L + E212D + E276N + W284Y +R320S + W408H + W439T + W469N + F473I + G476K 1.4 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + V206L + Q365S + K391A 2.0 H1* +N16H + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +K391A 1.6 Y160H + E212D + E276N + W284Y + R320S + Q365S + W408H +W439T + W469N + F473S + G476K 2.0 H1* + N54S + V56T + G109A + Q169E +Q172K + A174* + N195F + V206L + M246L + K391A 1.4 H1* + N54S + V56T +G109A + Y160H + Q169E + Q172K + A174* + N195F + V206L + K391A 2.3 H1* +N16Y + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L +K391A 2.4 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +M202L + V206L + K391A 2.5 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + G184K + N195F + V206L + T227N + K391A 1.3 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + M202L + V206L + Q365S + K391A4.0 H1* + l9M + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +M202L + V206LK + 391a 3.4 H1* + N54S + V56T + G109A + Y160H + Q169E +Q172K + A174* + N195F + M202L + V206L + K391A 3.1 H1* + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + M202L + V206L + R320S + K391A3.0 H1* + N16Y + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F +M202L + V206L + K391A 2.8 I9M + N54S + V56T + G109A + G149A + Q169E +Q172K + A174* + G182T + N195F + M202L + V206L + 3.1 Y295F + Q299Y +R320K + S323T + A339S + Q345R + Q365S + K391A + R458K I9M + N16Y +N54S + V56T + G109A + R116Y + Q118T + Q125S + Q136E + Y160H + Q169E +Q172K + 2.0 A174* + R181H + N195F + M202L + V206L + R310N + R320Q +Q365S + K391A + R400S + W408H + R444T + Q445S + Q466S H1* + I9M + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + M202L + V206L + Q365S +4.2 K391A

TABLE 8 FSW dataset II (45° C./8/KI55C-0.9 mg protein/wash)Modifications in SEQ ID NO: 2 IF SEQ ID NO: 2 1.0 Y295F 1.1 M246L 1.1N195F + Q365S 1.4 R171H + Q365C 1.1 V264I + A265G + Y267F 1.8 E86L +R171L + N306R + Q365S 1.3 V264I + Y267F + V291T + F328L + Q365S 1.6Y160H + E212D + E276N + W284Y + R320S + Q365S + W408H + W439T + W469N +F473S + G476K 1.3 I9M + R127N + N195F + Q365S 1.3 I9M + R171L + N195F +Q365S 1.9 N195F + G337E + Q365S + P375K + D377N 1.2 H1 * + N54S + V56T +G109A + R171H + N195F + V206L + K391A 1.1 H1* + N16Y + N54S + V56T +G109A + Q169E + Q172K + A174* + N195F + V206L + K391A 1.9 H1* + N16Y +N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + M202L + V206L +K391A 3.4 H1* + N54S + V56T + G109A + Y160H + Q169E + Q172K + A174* +N195F + V206L + K391A 1.8 H1* + N54S + V56T + G109A + Q169E + Q172K +A174* + N195F + M202L + V206L + K391A 3.3 H1* + N54S + V56T + G109A +Q169E + Q172K + A174* + N195F + V206L + Q365S + K391A 1.3 H1* + N54S +V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + Q365S + K391A 2.2H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + M202L +V206L + Q365S + K391A 3.7 H1* + I9M + N54S + V56T + G109A + G149A +Q169E + Q172K + A174* + G182T + N195F + M202L + 3.3 V206L + Y295F +Q299Y + R320K + S323T + A339S + Q345R + Q365S + K391A + R458K

The invention described and claimed herein is not to be limited in scopeby the specific aspects herein disclosed, since these aspects areintended as illustrations of several aspects of the invention. Anyequivalent aspects are intended to be within the scope of thisinvention. Indeed, various modifications of the invention in addition tothose shown and described herein will become apparent to those skilledin the art from the foregoing description. Such modifications are alsointended to fall within the scope of the appended claims. In the case ofconflict, the present disclosure including definitions will control.

1. An alpha-amylase variant of a parent alpha-amylase polypeptidecomprises a modification in one or more position corresponding toposition: 1, 2, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60,63, 81, 84, 86, 90, 98, 104, 109, 111, 113, 116, 118, 125, 127, 130,132, 133, 134, 135, 136, 139, 142, 144, 149, 158, 160, 163, 167, 169,170, 171, 172, 173, 174, 175, 176, 178, 181, 182, 186, 187, 195, 202,203, 204, 206, 209, 210, 212, T227, 235, 238, 246, 256, 259, 264, 265,266, 267, 269, 270, 272, 273, 274, 275, 276, 284, 286, 291, 293, 295,298, 299, 302, 303, 304, 306, 310, 311, 314, 315, 317, 319, 320, 323,328, 337, 339, 345, 357, 365, 377, 375, 385, 391, 395, 400, 406, 408,410, 431, 435, 439, 444, 445, 458, 465, 466, 469, 473, 476 and 481,using SEQ ID NO: 1 for numbering and wherein each modification isindependently a substitution, insertion, or deletion, and wherein saidvariant has at least 59% but less than 100% sequence identity to thepolypeptide of SEQ ID NOs: 1-17 and wherein said variant hasalpha-amylase activity.
 2. The alpha-amylase variant of claim 1, whereinsaid modification in one or more positions correspond to the following:H1, H2, G7, I9, Q11, N16, N19, N25, A37, W43, W48, N54, V56, Y58, G59,A60, L63, T81, E84, E86, R90, Q98, V104, G109, A111, F113, R116, Q118,Q125, R127, E130, S132, G133, T134, Y135, Q136, A139, G142, N144, G149,R158, Y160, D163, W167, Q169, S170, R171, Q172, L173, A174, N175, R176,Y178, R181, G182, A186, W187, N195, M202, Y203, A204, V206, D209, H210,E212, T227, L235, V238, M246, Q256, K259, V264, A265, E266, Y267, K269,N270, L272, G273, A274, L275, E276, W284, M286, V291, L293, Y295, Y298,Q299, N302, S303, S304, N306, R310, N311, N314, G315, L317, Q319, R320,S323, F328, G337, A339, Q345, D357, Q365, P377, D375, Q385, K391, Q395,R400, D406, W408, V410, S431, G435, W439, R444, Q445, R458, N465, Q466,W469, F473, G476 and V481, using SEQ ID NO: 1 for numbering and whereineach modification is independently a substitution, insertion, ordeletion, and wherein said variant has at least 59% but less than 100%sequence identity to the polypeptide of SEQ ID NOs: 1-17, and whereinsaid variant has alpha-amylase activity and wherein said variant havingan Improvement Factor (IF) of >1.0 for a measure of wash performancewhen compared to said parent polypeptide having alpha-amylase activityof SEQ ID NO: 1 or SEQ ID NO:
 2. 3. The alpha-amylase variant accordingto claim 1, wherein said variant comprises a deletion in two or morepositions corresponding to positions R181, G182, D183, and G184, usingSEQ ID NO: 1 for numbering.
 4. The alpha-amylase variant according toclaim 1, wherein said variant comprises one or more of the followingmodifications: H1*; H2*, G7A; I9M; Q11H; N16E; N16H; N16Y; N19D; N25K;N25M; N25T; A37V; W43Y; W48Y; N54Q; N54S; V56I; V56T; Y58F; G59A; A60S;A60T; L63F; T81A; E84Q; E86L; R90H; R90N; R90Q; Q98N; V104A; G109A;A111T; F113A; F113G; F113N; F113Q; F113T; F113Y; R116C; R116D; R116F;R116H; R116K; R116N; R116S; R116Y; Q118N; Q118T; Q125G; Q125S; R127A;R127F; R127H; R127L; R127N; R127T; E130D; E130F; E130I; E130K; E130L;E130N; E130S; E130T; E130V; S132A; S132D; S132F; S132L; S132M; S132P;S132T; G133D; T134C; T134E; T134N; T134P; T134R; T134S; T134W; T134Y;T134A; Y135H; Q136D; Q136E; Q136G; Q136N; Q136R; Q136S; A139T; G142H;N144H; G149A; R158C; R158H; R158K; R158Q; R158S; Y160D; Y160F; T160H;Y160N; D163H; W167*; Q169*; Q169E; Q169F; S170*; 5170A; R171*; R171H;R171K; R171L; R171Y; Q172*; Q172K; Q172N; Q172S; L173*; L173K; A174*;A174N; A174S; N175*; N175S; R176*; R176A; Y178F; R181*; R181A; R181C;R181E; R181G; R181H; R181K; R181N; R181Q; G182*; G182T; A186D; A186E;A186H; A186K; A186N; A186T; W187M; N195D; N195F; N195H; N195Q; N195Y;M202L; Y203L; A204V; V206L; D209N; H210K; H210N; E212D; T227K; T227N;L235I; V238A; M246I; M246L; M246T; M246V; Q256H; Q256N; K259G; K259H;V264A; V246I; V264T; A265G; E266V; Y267F; Y267H; Y267L; K269M; K269Q;K269R; N270G; N270P; L272I; G273V; A274K; A274S; L275A; L275I; L275V;E276N; W284R; W284Y; M286L; V291A; V291I; V291T; L293I; L293V; Y295F;Y298E; Y298N; Q299N; Q299Y; N302A; N302H; N302K; N302Q; 5303G; 5304G;5304Q; N306H; N306K; N306Q; N306R; N306Y; R310N; R310S; R310Q; N311K;N311R; N314Q; G315N; L317V; Q319H; R320K; R320Q; R320S; S323T; H323K;F328L; G337D; G337E; A339S; Q345N; Q345R; L357F; Q365A; Q365C; Q365E;Q365H; Q365K; Q365M; Q365S; P377K; P377T; D375H; D375N; D375Y; Q386L;K391A; Q395K; R400S; D406H; W408H; V410I; S431F; G435C; W439R; W439T;R444T; Q445S; R458K; N465G; Q466S; W469N; W469Y; F473I; F473P; F473S;G476K; and V481A, using SEQ ID NO: 1 for numbering.
 5. The variantaccording to claim 1, wherein said variant is selected from the groupconsisting of: A60T, Y135H, Q169E, W48Y, A174*, M246L, R90Q,Y203L+V206L, Q169F+S170A, S132D+G133D, N195F+Q365C, N195F+Q365S,N195F+Q365M, R127L+Q365S, R127N+Q365S, R127L+Q365C, N195F+Q365K,I9M+N195F, R171H+Q365C, Y267F+Q365S, F328L+Q365S, R171L+Q365S,N195F+M286L, T134P+N195F, S170*+R171*+A174*, V264I+A265G+Y267F,Q169*+S170*+A174*, M246L+L275V+Q365S, V264I+V291I+Q365S,V264I+V291T+Q365K, V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C,I9M+N195F+Q365S, V264I+Y267F+Q365K, R127L+M246V+Q365S, A60S+R171Y+Q365S,R127N+M246T+L275V, V104A+N195F+Q365S, V264I+F328L+Q365S,R171H+N302H+Q365S, V264I+Y267F+Q365S, A174*+N175*+Y178F,V264I+A265G+Q365C, R171L+N302H+Q365C, R171L+M246V+Q365C,R171Y+M246L+Q365S, V264T+Y267F+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, R127N+M246L+L275V+Q365S,R171H+M246L+L275V+Q365S, R127H+M246I+Q365C+V481A,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, W187M+N195F+H210K+Q365S,R127H+M246T+L275A+Q365S, I9M+A111T+N195F+Q365S, I9M+Y58F+N195F+Q365S,V264T+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F+Q365C, V264I+Y267F+V291A+F328L+Q365S,R127N+R171H+M246L+L275V+Q365S, V264I+A265G+V291A+F328L+Q365S,R127N+M246I+A265G+Y267H+Q365S, E130I+T134A+R181N+N195F+Q365S,R127N+M246L+L275V+N306R+Q365S, I9M+N195F+V206L+D209N+Q365S,R116S+S132F+R181N+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,A37V+V264I+Y267F+F328L+Q365C, Q11H+V264I+Y267F+F328L+Q365C,V264I+Y267F+V291T+F328L+Q365S, Q169*+R171*+Q172*+L173*+A174*,R116Y+S132P+Q136N+N195F+Q365S, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+Y267F+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,E130D+T134Y+R181H+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N195F+G337D+Q365S+P375K+D377N, R127N+R171L+M246L+L275V+Q365S,N195F+G337E+Q365S+P375K+D377N, Q11H+V264I+A265G+Y267F+Q365C,R158Q+Y160N+W187M+N195F+Q365S, V264I+Y267F+Q365K+W408H+V410I,R127N+R171Y+M246L+L275V+Q365S, N54Q+F113T+R171H+N302A+Q365C,V264A+Y267F+V291T+F328L+Q365C, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R116C+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R116C+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,V264I+A265G+Y267F+V291I+F328L+Q365S, N16E+N195F+G337D+Q365S+P375K+D377N,V264I+A265G+Y267F+F328L+Q365S+Q395K,E130N+T134C+R181Q+A186N+N195F+Q365S,V264I+A265G+Y267F+V291A+F328L+Q365C,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S, A186D+N195F,A60S+R171Y+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130D+T134Y+R181H+N195F+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S, E130I+T134A+R181N+N195F+Q365S,E130I+T134A+R181N+N195F+Q365S, E130L+T134N+R181W+A186T+N195F+Q365S,E130N+T134C+R181Q+A186N+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S, E86L+R171L+N306R+Q365S,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,E86L+R171L+N306R+Q365S, F113Q+R116H, F113T+R171K+G273V, F328L+Q365S,G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y,H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202 L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A, H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q125G+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K269M+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302 K+S303G+K391A, H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A, H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+G184K+N195F+Q365S,I9M+N195F+K269Q+A274K+Q365S, I9M+N195F+N270G+Q365S,I9M+N195F+N270P+Q365S, I9M+N195F+Q365C, I9M+N195F+V206L+D209N+Q365S,I9M+N195F+V291A+Q365S, I9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S,I9M+G184K+N195F+Q365S,I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,I9M+N195F+Q365C,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,I9M+R127N+N195F+Q365S, I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+N195F+Q365S, M202L, M246L+L275V+Q365S+D406H, M246L,M246L+L275V+Q365S, M246L+L275V+Q365S+D406H,N16E+N195F+G337D+Q365S+P375K+D377N, N16E+N195F+G337E+Q365S+P375T+D377H,N16E+N195F+G337E+Q365S+P375T+D377H,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S, N195F+G337D+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+Q365S, N54Q+F113T+R171H+N302A+Q365C,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Q11H+V264I+A265G+Q365C, Q11H+V264I+A265G+Y267F+Q365C,Q11H+V264I+Y267F+F328L+Q365C, Q11H+Y267F+Q365C, Q11H+V264I+A265G+Q365C,Q169*+S170*+A174*, Q169*+S170*+R171*+A174*,Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,R116K+S132L+Q136R+R181C+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116S+S132F+R181N+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S, R116Y+S132P+Q136N+N195F+Q365S,R127H+L235I+Q365S+G435C, R127L+M246V+Q365S, R127L+Q365C, R127L+Q365S,R127L, R127N+M246L+L275V+Q365S, R127N+Q365S,R127N+R171L+M246L+L275V+Q365S, R127N+R171H+M246L+L275V+Q365S,R171H+Q365C, R171H+M246L+L275V+Q365S, R171H+N302H+Q365S, R171H,R171H+Q365C, R171L+N302H+Q365C, R171L+Q365S, R171Y++M246L+Q365S,R171Y+N306K, R181S+G184S, V104A+N195F+Q365S,V264A+Y267F+V291T+F328L+Q365C, V264I+A265G+V291A+F328L+Q365S,V264I+A265G+V291A+Q365C, V264I+A265G+Y267F+F328L+Q365S+Q395K,V264I+A265G+Y267F+F328L+Q365S, V264I+A265G+Y267F+V291I+F328L+Q365S,V264I+A265G+Y267F, V264I+F328L+Q365C, V264I+F328L+Q365S, V264I+L357F,V264I+Q365C, V264I+V291I+Q365S, V264I+V291T+Q365K,V264I+Y267F+F328L+Q365C, V264I+Y267F+F328L+Q365K,V264I+Y267F+F328L+Q365S, V264I+Y267F+Q365K+W408H+V410I,V264I+Y267F+Q365K, V264I+Y267F+Q365S, V264I+Y267F+V291A+F328L+Q365C,V264I+Y267F+V291A+F328L+Q365S, V264I+Y267F+V291T+F328L+Q365S,V264I+A265G+Y267F, V264I+F328L+Q365C, V264I+Y267F+F328L+Q365K,V264I+Y267F+V291T+F328L+Q365S, V264T+A265G+Y267F+V291A+F328L+Q365C,V264T+A265G+Y267L+F328L+Q365C, V264T+V291A+F328L+Q365S,V264T+Y267F+Q365S, V264T+Y267L+F328L+Q365C,V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6K,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,W187M+N195F+H210K+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K, Y160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K, Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y267F+Q365S, Y267F+V291A+F328L+Q365C, Y267F+V291A+F328L+Q365S,Y267F+Q365S, Y267F+V291A+F328L+Q365S, Y295F, using SEQ ID NO: 1 fornumbering and wherein said variant Improvement Factor (IF) of at least1.1 in Model A detergent composition and/or ADW detergent compositionwith bleach when compared to said parent alpha-amylase and wherein saidparent alpha-amylase has amino acid sequence as shown in SEQ ID NO: 2.6. The variant according to claim 1, wherein said variant is selectedfrom the group consisting of: A60T, Y135H, Q169E, W48Y, A174*, M246L,R90Q, Y203L+V206L, Q169F+S170A, S132D+G133D, N195F+Q365C, N195F+Q365S,N195F+Q365M, R127L+Q365S, R127N+Q365S, R127L+Q365C, N195F+Q365K,I9M+N195F, R171H+Q365C, Y267F+Q365S, F328L+Q365S, R171L+Q365S,N195F+M286L, T134P+N195F, S170*+R171*+A174*, V264I+A265G+Y267F,Q169*+S170*+A174*, M246L+L275V+Q365S, V264I+V291I+Q365S,V264I+V291T+Q365K, V264I+F328L+Q365C, I9M+N195F+Q365C, Q11H+Y267F+Q365C,I9M+N195F+Q365S, V264I+Y267F+Q365K, R127L+M246V+Q365S, A60S+R171Y+Q365S,R127N+M246T+L275V, V104A+N195F+Q365S, V264I+F328L+Q365S,R171H+N302H+Q365S, V264I+Y267F+Q365S, A174*+N175*+Y178F,V264I+A265G+Q365C, R171L+N302H+Q365C, R171L+M246V+Q365C,R171Y+M246L+Q365S, V264T+Y267F+Q365S, I9M+Y160H+N195F+Q365S,I9M+R127N+N195F+Q365S, R127H+L235I+Q365S+G435C, I9M+R171H+N195F+Q365S,I9M+A186H+N195F+Q365S, I9M+R171L+N195F+Q365S, Q169*+S170*+R171*+A174*,M246L+L275V+Q365S+D406H, I9M+G184K+N195F+Q365S, R127N+M246L+L275V+Q365S,R171H+M246L+L275V+Q365S, R127H+M246I+Q365C+V481A,Y267F+V291A+F328L+Q365C, I9M+A186E+N195F+Q365S, E86L+R171L+N306R+Q365S,V264I+Y267F+F328L+Q365S, I9M+N195F+V291A+Q365S, R127L+L235I+M246I+Q365C,Y267F+V291A+F328L+Q365S, V264I+Y267F+F328L+Q365K,Q11H+V264I+A265G+Q365C, W187M+N195F+H210K+Q365S,R127H+M246T+L275A+Q365S, I9M+A111T+N195F+Q365S I9M+Y58F+N195F+Q365S,V264T+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F+Q365C, V264I+Y267F+V291A+F328L+Q365S,R127N+R171H+M246L+L275V+Q365S, V264I+A265G+V291A+F328L+Q365S,R127N+M246I+A265G+Y267H+Q365S, E130I+T134A+R181N+N195F+Q365S,R127N+M246L+L275V+N306R+Q365S, I9M+N195F+V206L+D209N+Q365S,R116S+S132F+R181N+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,A37V+V264I+Y267F+F328L+Q365C, Q11H+V264I+Y267F+F328L+Q365C,V264I+Y267F+V291T+F328L+Q365S, Q169*+R171*+Q172*+L173*+A174*,R116Y+S132P+Q136N+N195F+Q365S, N195F+G337D+Q365S+P375K+D377Y,V264I+A265G+Y267F+F328L+Q365S, I9M+N195F+K269Q+A274K+Q365S,E130D+T134Y+R181H+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,N195F+G337D+Q365S+P375K+D377N, R127N+R171L+M246L+L275V+Q365S,N195F+G337E+Q365S+P375K+D377N, Q11H+V264I+A265G+Y267F+Q365C,R158Q+Y160N+W187M+N195F+Q365S, V264I+Y267F+Q365K+W408H+V410I,R127N+R171Y+M246L+L275V+Q365S, N54Q+F113T+R171H+N302A+Q365C,V264A+Y267F+V291T+F328L+Q365C, Q169*+S170*+R171*+Q172*+L173*+A174*,R116K+S132L+Q136R+R181C+N195F+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C,E130N+T134S+R181N+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S, E130D+T134W+R181KA186E+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S,R1160+S132A+Q136S+R181A+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+Q365S,R127H+R171H+M246L+L275V+N306R+Q365S,E130L+T134N+R181W+A186T+N195F+Q365S,R1160+S132M+Q136S+R181Q+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S,R116S+S132L+Q136E+R181G+N195F+Q365S, N16E+N195F+G337E+Q365S+P375T+D377H,V264I+A265G+Y267F+V291I+F328L+Q365S, N16E+N195F+G337D+Q365S+P375K+D377N,V264I+A265G+Y267F+F328L+Q365S+Q395K,E130N+T1340+R181Q+A186N+N195F+Q365S,V264I+A265G+Y267F+V291A+F328L+Q365C,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,E130K+S132T+T134Y+R181T+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,W167*+Q169*+S170*+R171*+Q172*+L173*+A174*+N175*+R176*,H1*+G59A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y58F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+G109A+Q169E+Q172K+A174*+N195F+V206L+L293I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+D209N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195D+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195H+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275I+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337E+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25K+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R127T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D209N+K391A,H1*+N54S+V56T+G109A+R127N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+L63F+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L275V+K391A,H1*+N19D+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,A60S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+R171L+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G337D+K391A,H1*+N25M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+T81A+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N25T+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+R90N+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R158C+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L293V+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L272I+K391A,H1*+N54S+V56T+G109A+R158H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+G109A+K391A+N16Y+N54S+V56T+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+R158S+Q169E+Q172K+A174*+N195F+V206L+K391A,R127N+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+A274S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186E+N195F+V206L+K391A,H1*+N54S+V56T+R90H+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q172S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365C+K391A,H1*+N54S+V56T+G109A+R116N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302H+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365E+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+A186H+N195F+V206L+K391A,H1*+N54S+V56T+A60S+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R181S+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172S+A174*+N175S+N195F+V206L+K391A,H1*+N54S+V56T+E86L+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302K+K391A,H1*+N54S+V56T+G109A+R127L+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+R171Y+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V291A+K391A,H1*+N54S+V56T+G109A+R116F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,Y160H+R171L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+G109A+K391A+N54S+V56T+Y160H+Q169E+Q172K+A174*+N195F+V206L,H1*+N54S+V56T+G109A+Q118N+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+T134P+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*N195F+V206L+Q299Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365A+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q299N+K391A,Y160H+E212D+L275V+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306Q+K391A,H1*+W43Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311R+K391A,Y160H+E212D+M246L+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+E84Q+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R158K+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,N54S+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q125G+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N311K+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K269M+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+K391A,Y160H+E212D+K259G+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+A174S+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+N195Y+E212 D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+Q169E+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A, Y160H+E212D+E276N+W284Y+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,G7A+F113N+R116N+E276N+S304G+R320S+Q345N+W439R+N465G+Q466S+W469Y,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+D377H+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+V238A+K391A,Y160H+N195F+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+Q256N+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365H+K391A,Y160H+N195Q+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+R171Y+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,G109A+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212 D+E276N+W284Y+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+R127F+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+L173K+A174*+N175S+N195F+V206L+K391A,Y160H+E212D+E276N+W284Y+S304G+N306R+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+G142H+N144H+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N302Q+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+T227K+K269R+K391A,H1*+N54S+V56I+G109A+R127A+Q169E+Q172K+A174*+N195F+V206L+Q385L+K391A,Y160H+E212D+Q256H+K259H+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+G315N+Q385L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172N+A174*+N175S+N195F+V206L+K391A+S431F,Y160H+E212D+E276N+W284Y+S304G+N306Y+R320S+W408H+W439T+W469N+F473S+G476K,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+L235I+V238A+K391A,H1*+N54S+V56T+G109A+Q172S+L173K+A174S+N175*+R176A+N195F+V206L+K391A,H1*+N54S+V56T+G109A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,V56T+Y160H+A174N+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G47 6K,H1*+N54S+V56T+R90Q+G109A+Q169E+Q172K+A174*+N195F+V206L+E266V+Q385L+K391A, H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V20+6L+Q385L+K391A,H1*+N54S+V56T+G109A+F113A+R116D+Q118T+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Y298E+N302K+S303G+K391A,H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+N195F+V206L+N314Q+Q385L+K391A,H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S, using SEQ ID NO: 1 for numbering and wherein saidvariant Improvement Factor (IF) of at least 1.1, preferably at least1.2, such as at least 1.3, such as at least 1.4, such as at least 1.5,such as at least 1.6, such as at least 1.7, such as at least 1.8, suchas at least 1.9, such as at least 2.0, such as at least 2.2, such as atleast 2.4, such as at least 2.6, such as at least 2.8, such as at least3.0, such as at least 3.2, such as at least 3.4, such as at least 3.6,such as at least 3.8, or such as at least 4.0 in Model A detergentcomposition when compared to said parent alpha-amylase and wherein saidparent alpha-amylase has amino acid sequence as shown in SEQ ID NO: 2.7. The variant according to claim 1, wherein said variant is selectedfrom the group consisting of: A139T+R158S+Y160D+W187M+N195F+H210N+Q365S,A186D+N195F, A60S+R171Y+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130D+T134Y+R181H+N195F+Q365S, E130D+T134W+R181K+A186E+N195F+Q365S,E130F+T134R+R181S+A186D+N195F+Q365S, E130I+T134A+R181N+N195F+Q365S,E130I+T134A+R181N+N195F+Q365S, E130L+T134N+R181W+A186T+N195F+Q365S,E130N+T134C+R181Q+A186N+N195F+Q365S,E130S+T134E+R181S+A186T+N195F+Q365S, E130T+T134Y+A186K+N195F+Q365S,E130V+T134N+R181Y+A186K+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S,E130Y+S132T+T134S+R181E+A186D+N195F+Q365S, E86L+R171L+N306R+Q365S,E86L+Y160H+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,E86L+R171L+N306R+Q365S, F113Q+R116H, F113T+R171K+G273V, F328L+Q365S,H1*+I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S,H1*+I9M+N54S+V56T+G109A+F113G+R116H+D163H+Q169E+R171H+Q172K+A174S+G182T+N195F+M202L+V206L+M246L+L275V+A288V+L317V+Q319H+H324K+Q365S+K391A,H1*+I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+W284Y+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+N306R+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+V206L+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+I9M+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16H+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N16Y+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G184K+N195F+V206L+T227N+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+M202L+V206L+R320S+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+M246L+K391A,H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+Q365S+K391A,H1*+N54S+V56T+G109A+R171H+N195F+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+M202L+V206L+K391A,H1*+N54S+V56T+G109A+Y160H+Q169E+Q172K+A174*+N195F+V206L+K391A,I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S, I9M+G184K+N195F+Q365S,I9M+N195F+K269Q+A274K+Q365S, I9M+N195F+N270G+Q365S,I9M+N195F+N270P+Q365S, I9M+N195F+Q365C, I9M+N195F+V206L+D209N+Q365S,I9M+N195F+V291A+Q365S, I9M+R127N+N195F+Q365S I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,I9M+Y160H+N195F+Q365S, I9M+A186E+N195F+Q365S, I9M+A186H+N195F+Q365S,I9M+G184K+N195F+Q365S,I9M+N16Y+N54S+V56T+G109A+R116Y+Q118T+Q125S+Q136E+Y160H+Q169E+Q172K+A174*+R181H+N195F+M202L+V206L+R310N+R320Q+Q365S+K391A+R400S+W408H+R444T+Q445S+Q466S, I9M+N195F+Q365C,I9M+N54S+V56T+G109A+G149A+Q169E+Q172K+A174*+G182T+N195F+M202L+V206L+Y295F+Q299Y+R320K+S323T+A339S+Q345R+Q365S+K391A+R458K,I9M+R127N+N195F+Q365S, I9M+R171H+N195F+Q365S,I9M+R171L+N195F+M246L+K269M+W284R+Y298N+Q365S, I9M+R171L+N195F+Q365S,I9M+Y160H+N195F+Q365S, M202L, M246L, M246L+L275V+Q365S+D406H,M246L+L275V+Q365S, M246L+L275V+Q365S+D406H,N16E+N195F+G337D+Q365S+P375K+D377N, N16E+N195F+G337E+Q365S+P375T+D377H,N16E+N195F+G337E+Q365S+P375T+D377H,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S,N16Y+Y160H+N195F+L275I+S304G+G337E+Q365S, N195F+G337D+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+G337D+Q365S+P375K+D377Y, N195F+G337E+Q365S+P375K+D377N,N195F+Q365S, N54Q+F113T+R171H+N302A+Q365C, Q11H+V264I+A265G+Q365C,Q11H+V264I+A265G+Y267F+Q365C, Q11H+V264I+Y267F+F328L+Q365C,Q11H+Y267F+Q365C, Q11H+V264I+A265G+Q365C, Q169*+S170*+A174*,Q169*+S170*+R171*+A174*, Q98N+F113Y+R158Q+Y160F+S304Q+R320S+W439R+F473P,R116K+S132L+Q136R+R181C+N195F+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116K+S132P+Q136D+R181Q+N195F+A204V+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116N+S132M+Q136R+N195F+Q365S,R116N+S132A+Q136D+R181K+N195F+Q365S, R116S+S132F+R181N+N195F+Q365S,R116S+Y135H+Q136G+R181T+N195F+Q365S, R116Y+S132P+Q136N+N195F+Q365S,R127H+L235I+Q365S+G435C, R127L, R127L+M246V+Q365S, R127L+Q365C,R127L+Q365S, R127N+M246L+L275V+Q365S, R127N+Q365S,R127N+R171L+M246L+L275V+Q365S, R127N+R171H+M246L+L275V+Q365S, R171H,R171H+Q365C, R171H+M246L+L275V+Q365S, R171H+N302H+Q365S, R171H+Q365C,R171L+N302H+Q365C, R171L+Q365S, R171Y++M246L+Q365S, R171Y+N306K,R181S+G184S, V104A+N195F+Q365S, V264A+Y267F+V291T+F328L+Q365C,V264I+A265G+V291A+F328L+Q365S, V264I+A265G+V291A+Q365C,V264I+A265G+Y267F, V264I+A265G+Y267F+F328L+Q365S,V264I+A265G+Y267F+F328L+Q365S+Q395K,V264I+A265G+Y267F+V291I+F328L+Q365S, V264I+F328L+Q365C,V264I+F328L+Q365S, V264I+L357F, V264I+Q365C, V264I+V291I+Q365S,V264I+V291T+Q365K, V264I+Y267F+F328L+Q365C, V264I+Y267F+F328L+Q365K,V264I+Y267F+F328L+Q365S, V264I+Y267F+Q365K,V264I+Y267F+Q365K+W408H+V410I, V264I+Y267F+Q365S,V264I+Y267F+V291A+F328L+Q365C, V264I+Y267F+V291A+F328L+Q365S,V264I+Y267F+V291T+F328L+Q365S, V264I+A265G+Y267F, V264I+F328L+Q365C,V264I+Y267F+F328L+Q365K, V264I+Y267F+V291T+F328L+Q365S,V264T+A265G+Y267F+V291A+F328L+Q365C, V264T+A265G+Y267L+F328L+Q365C,V264T+V291A+F328L+Q365S, V264T+Y267F+Q365S, V264T+Y267L+F328L+Q365C,W187M+N195F+H210K+Q365S,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473S+G476K,Y160H+E212D+E276N+W284Y+R320S+Q365S+W408H+W439T+W469N+F473S+G476K,Y160H+M202L+E212D+E276N+W284Y+R320S+W408H+W439T+W469N+F473I+G476K,Y267F+Q365S, Y267F+V291A+F328L+Q365C, Y267F+V291A+F328L+Q365S,Y267F+Q365S, Y267F+V291A+F328L+Q365S, Y295F, using SEQ ID NO: 1 fornumbering and wherein said variant Improvement Factor (IF) of at least1.1 in ADW detergent composition with bleach when compared to saidparent alpha-amylase and wherein said parent alpha-amylase has aminoacid sequence as shown in SEQ ID NO:
 2. 8. A composition comprising thevariant according to claim 1 and at least one additional activecomponent.
 9. The composition according to claim 8, wherein thecomposition is a detergent and wherein the detergent composition is aliquid laundry detergent composition, a powder laundry detergentcomposition, a liquid dishwash detergent composition, or a powderdishwash detergent composition.
 10. The composition according to claim8, wherein said composition is a liquid or powder automatic dishwashing(ADW) detergent composition.
 11. (canceled)
 12. A method for removal ofsoils from fabric or hard surfaces comprising contacting the fabric orhard surfaces contaminated with soils with a composition according toclaim
 8. 13. A method of dishwashing in an automatic dishwashing machineusing a composition according to claim 8, comprising the steps of addingsaid detergent composition in a detergent composition compartment insaid automatic dishwashing machine, and releasing said detergentcomposition during a main-wash cycle.
 14. A method of laundering in anautomatic laundering machine using a composition according to claim 8,comprising the steps of adding said detergent composition in a detergentcomposition compartment in said automatic laundering machine, andreleasing said detergent composition during a main wash cycle.
 15. Amethod of improving the performance, such as wash performance, of aparent alpha-amylase having the amino acid sequence of SEQ ID NO: 2, orhaving at least 59% sequence identity hereto, said method comprising thesteps of: a) introducing a substitution and/or deletion at two or morepositions corresponding to positions R181, G182, D183, and G184, usingSEQ ID NO: 1 for numbering; and b) introducing a deletion and/orsubstitution at one or more positions selected from the group consistingof: 1, 7, 9, 11, 16, 19, 25, 37, 43, 48, 54, 56, 58, 59, 60, 63, 81, 84,86, 90, 98, 104, 109, 111, 113, 116, 118, 125, 127, 130, 132, 133, 134,135, 136, 139, 142, 144, 149, 158, 160, 163, 167, 169, 170, 171, 172,173, 174, 175, 176, 178, 181, 182, 186, 187, 195, 202, 203, 204, 206,209, 210, 212, T227, 235, 238, 246, 256, 259, 264, 265, 266, 267, 269,270, 272, 273, 274, 275, 276, 284, 286, 291, 293, 295, 298, 299, 302,303, 304, 306, 310, 311, 314, 315, 317, 319, 320, 323, 328, 337, 339,345, 357, 365, 377, 375, 385, 391, 395, 400, 406, 408, 410, 431, 435,439, 444, 445, 458, 465, 466, 469, 473, 476 and/or 481; using SEQ ID NO:1 for numbering and said method thereby providing an alpha-amylasevariant of said parent alpha-amylase, wherein said variant has at least59% but less than 100% sequence identity to the amino acid sequence asset forth in SEQ ID NOs: 1-17, and wherein said variant hasalpha-amylase activity and improved performance as compared to saidparent alpha-amylase.